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La3+ uptake and its effect on the cytoskeleton in root protoplasts of Zea mays L.

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Abstract

La3+ ions are known to antagonize Ca2+ and are used as a Ca2+ channel blocker but little is known on the direct effects of La3+. Micromolar La3+ concentrations promoted root growth while higher concentrations were inhibitory. The uptake of La3+ in maize root protoplasts revealed a membrane binding component (0.14 and 0.44 pmol min−1 protoplast−1 for 100 and 1,000 μM La3+) followed by a slower concentration and time-dependent uptake. Uptake was reduced by Ca2+, but had no substantial effect on other ions. La3+ shifted microtubule organization from random to parallel but caused aggregation of microfilaments. Our data suggest that La3+ is taken up into plant cells and affects growth via stabilization of the cytoskeleton.

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Abbreviations

CW :

Cell wall

ICP-OES :

Inductively coupled plasma spectrometry

LAN :

Lanthanum

MF :

Microfilament

MT :

Microtubule

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Acknowledgements

We thank Dr. Susan Mopper for assistance with the statistical analyses and Dr. Tom Pesacreta for assistance with the microscopy. This work was supported by NASA (grant no. NAG10-0190) and the Graduate Student Organization of UL Lafayette.

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  1. Department of Biology, University of Louisiana at Lafayette, Lafayette, LA 70504, USA

    Min Liu & Karl H. Hasenstein

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  1. Min Liu
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  2. Karl H. Hasenstein
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Correspondence to Karl H. Hasenstein.

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Liu, M., Hasenstein, K.H. La3+ uptake and its effect on the cytoskeleton in root protoplasts of Zea mays L.. Planta 220, 658–666 (2005). https://doi.org/10.1007/s00425-004-1379-2

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