Abstract
The superior quality of Stephania dentifolia is determined by the presence of desirable multiple alkaloids and the case with which one can multiply the healthy and superior source plants in vitro is an important requisite. Other factors related to successful propagation strategies are the cost of micropropagation, biomass production, and its medicinal content. In the present study, micropropagation was carried out from nodal explants collected from three parts of healthy S. dentifolia vines comprising the lower, middle and upper parts. The upper material was the most tender and the micropropagation effect was the best. The other two parts could also be used in micropropagation because of their lower pollution rate and higher induction rate. The tender stem section soaked in 70% alcohol for 15 s and then in 2.5% NaClO solution for 10 min, of which the pollution rate was 10.6%. The explants cultured on MS medium supplemented with 1.0 mg L−1benzyl adenine (BA), 0.2 mg L−1 naphthalene acetic acid (NAA), 0.5 mg L−1kinetin 0.1 mg L−1 thidiazuron and 2 g L−1 powdered activated carbon (PAC) resulted in the formation of maximum induction rate (88.32%), and microshoots were elongated (4 cm) within 3 weeks of culture period. Enhanced axillary bud proliferation and production of mass number of micro shoots was achieved by the continuous subculture in MS medium containing 0.1 mg L−1 NAA and 2 g L−1 PAC. In vitro regenerated shoots were rooted on 1/2 MS with 0.5 mg L−1 indole-3-butyric acid (IBA). After successful acclimatization, rooted plants were transferred to poly-ethylene pots containing a soil: perlite (3:1 v/v) mixture with 100% of survival rate. We improved the efficiency and reduced the costs of micropropagation of S. dentifolia via artificial cultivation.
Key message
In vitro regenerated shoots were rooted on 1/2 MS with 0.5 mg L-1 indole-3-butyric acid (IBA).
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Acknowledgements
This work was jointly supported by National Natural Science Foundation of China (NO. 32160415); Biological Quality Engineering Project (NO. 503190106); Yunnan Provincial Joint Special Project for Basic Research in Agriculture (202101BD070001-114); Yunnan Province Xingdian Talents Support Plan and Yunnan Provincial Department of Education Fund for Scientific Research (No. 2020J0413).
Funding
Natural Science Foundation of China (Grant no. 32160415), Biological Quality Engineering Project (Grant no. 503190106), Yunnan Provincial Joint Special Project for Basic Research in Agriculture (Grant no. 202101BD070001-114), Yunnan Province Xingdian Talents Support Plan, Scientific Research Foundation for Returned Scholars of Ministry of Education (Grant no. 2020J0413).
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Yu, L., Duan, Y. & Zhan, H. An improved micropropagation of a medicinal plant Stephania dentifolia. Plant Cell Tiss Organ Cult 153, 219–224 (2023). https://doi.org/10.1007/s11240-022-02443-w
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DOI: https://doi.org/10.1007/s11240-022-02443-w