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Involvement of DNA methylation in tree development and micropropagation

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Abstract

Genes constitute only a small portion of the total genome and precisely controlling their expression represents a substantial problem for their regulation. Furthermore, non-coding DNA, that contains introns repetitive elements and active transposable elements, demands effective mechanisms to silence it long-term. Cell differentiation and development are controlled through temporal and spatial activation and silencing of specific genes. These patterns of gene expression must remain stable for many cell generations and last or change when inductive developmental signals have disappeared or new ones induce new programmes.

What turns genes on and off? Among others, gene regulation is controlled by epigenetic mechanisms, defined as any gene-regulating activity that does not also involve changes in the DNA code and is capable of persisting. It has become apparent that epigenetic control of transcription is mediated through specific states of the chromatin structure. Associations of specific chromosomal proteins, posttranslational histone modifications and DNA methylation are some of the epigenetic mechanisms that are involved in controlling chromatin states. DNA methylation research can be approached from several standpoints, since there is a wide range of techniques available to study the occurrence and localisation of methyldeoxycytosine in the genome. Several studies dealing with DNA methylation in relation to tree development, microproprogation and somaclonal variation will be presented, with the final aim of demonstrating that DNA methylation levels are hallmarks for growing seasonal periods and are related to open windows of competence in plants.

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Acknowledgements

The financial support needed to guarantee our progress into the insight of ageing, phase-change, and reinvigoration, as well as the progress being made in the research for quality markers has come from EU Projects FAIR3-CT96-1445, INCO 10063, and MCT-AGL2000-2126, AGL 2004-00810 Spanish National Projects. The Spanish M.E.C.D. supported fellowships of all young researchers. We would also like to thank Ms. Priscilla Chase for her linguistic revision of the text.

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  1. Plant Physiology, Epiphysage Research Group, B.O.S. Department, Faculty of Biology, University of Oviedo, C/ Cat. Rodrigo Uría, s/n, Oviedo, 33071, Spain

    Luis Valledor, Rodrigo Hasbún, Mónica Meijón, Jose Luis Rodríguez, Estrella Santamaría, Marcos Viejo, Maria Jesús Cañal & Roberto Rodríguez

  2. Asturias Institute of Biotechnology (Associated with CSIC), Asturias, Spain

    Luis Valledor, Rodrigo Hasbún, Mónica Meijón, Jose Luis Rodríguez, Estrella Santamaría, Maria Jesús Cañal & Roberto Rodríguez

  3. SERIDA, Asturias Service of Agricultural Research, Ctra, Ctra. Oviedo s/n, Villaviciosa, 33300, Spain

    Mónica Meijón & Isabel Feito

  4. Cancer Epigenetics Laboratory, Molecular Pathology Programme, National Cancer Centre (CNIO), c/ Melchor Fernández Almagro, no 3, 28029, Madrid, Spain

    Maria Berdasco & Mario F. Fraga

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  1. Luis Valledor
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Valledor, L., Hasbún, R., Meijón, M. et al. Involvement of DNA methylation in tree development and micropropagation. Plant Cell Tiss Organ Cult 91, 75–86 (2007). https://doi.org/10.1007/s11240-007-9262-z

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