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Identification of differentially expressed cDNA sequences and histological characteristics of Hevea brasiliensis calli in relation to their embryogenic and regenerative capacities

  • Cell Biology and Morphogenesis
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Abstract

Different friable Hevea callus lines from the same genotype can display different embryogenic and regenerative potentials. These lines can not be distinguished on the basis of macroscopic criteria. A histological and molecular study was undertaken to characterize the differences existing between five callus lines with different potentials. The genes differentially expressed during induction were analyzed using the differential display (DD-RT) technique. Twenty-eight cDNAs were found to be differentially expressed during induction in the embryogenic regenerating line (ER). Embryogenic nodules were formed earlier in the ER lines than in the embryogenic non-regenerating lines and were completely absent in the non-embryogenic line. Of these 28 cDNAs, five could be used to distinguish between calli prior to induction, thereby enabling an early diagnosis of friable Hevea callus embryogenic potential.

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Fig. 1 A–C
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Abbreviations

DD-RT::

Differential display technique

ENR::

Embryogenic non-regenerating line

ER::

Embryogenic regenerating line

NE::

Non-regenerating line

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Acknowledgements

We would like to thank Dr. Valérie Pujade-Renaud for supplying the cDNA clone of glutamine synthetase (GS) and Dr. Marie-Françoise Gautier (UMR GACA, INRA-Montpellier) for supplying the cDNA clone of the small subunit of soft wheat rubisco. Our thanks to Jacques Escoute for his assistance and technical supervision of the histology. We would also like to thank Lolita Triaire and Evelyne Dechamp for their technical assistance, and Peter Biggins for the English translation.

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Authors and Affiliations

  1. CIRAD, Avenue Agropolis, TA 80/03, 34398, Montpellier Cedex 5, France

    E. Charbit, T. Legavre, L. Lardet, N. Ferrière & M. P. Carron

  2. INRA-Unité de Biochimie et Biologie Moléculaire des Céréales, 2, Place Viala, 34060, Montpellier Cedex 01, France

    E. Bourgeois

Authors
  1. E. Charbit
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  2. T. Legavre
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  3. L. Lardet
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  4. E. Bourgeois
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  5. N. Ferrière
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  6. M. P. Carron
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Corresponding author

Correspondence to M. P. Carron.

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Communicated by P. Debergh

This work was funded by a grant from the French Ministry of National Education, Research and Technology and also by the Centre de Coopération International en Recherche Agronomique pour le Développement, Département des Cultures Pérennes (CIRAD-CP) at Montpellier-France.

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Charbit, E., Legavre, T., Lardet, L. et al. Identification of differentially expressed cDNA sequences and histological characteristics of Hevea brasiliensis calli in relation to their embryogenic and regenerative capacities. Plant Cell Rep 22, 539–548 (2004). https://doi.org/10.1007/s00299-003-0737-z

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  • DOI: https://doi.org/10.1007/s00299-003-0737-z

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