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Production and characterization of somatic hybrids between Brassica napus and Raphanus sativus

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Abstract

Intergeneric somatic hybridization between Brassica napus and Raphanus sativus was carried out to enrich gene pool of B. napus. Twelve somatic hybrids were produced via PEG-mediated protoplast fusion between B. napus and R. sativus. The hybridity was confirmed by morphological observation and molecular marker analysis. Hybrid progenies (BC1) were obtained via backcrosses with B. napus. Behaviour of R. sativus chromosomes in a B. napus background in the F1 and BC1 plants was revealed by genomic in situ hybridization (GISH). The potential of somatic hybridization to enrich the suitable gene pool for rapeseed breeding is discussed.

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Abbreviations

BCN :

Beet cyst nematode

CAPS :

Cleaved amplified polymorphic sequence

FAE1 :

Fatty acid elongase

GISH :

Genomic in situ hybridization

PMCs :

Pollen mother cells

SSR :

Simple sequence repeats

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Acknowledgement

This work was supported by the National Natural Science Foundation of China (30571175), the Natural Science Foundation of Jiangsu Province (BK2005048) and Fachagentur Nachwachsende Rohstoffe of Germany (FNR 22014601).

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Authors and Affiliations

  1. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, 225009, China

    Y. P. Wang

  2. Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, Groß Lüsewitz, 18190, Germany

    K. Sonntag, E. Rudloff, I. Groeneveld & J. Gramenz

  3. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    C. C. Chu

Authors
  1. Y. P. Wang
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  2. K. Sonntag
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  3. E. Rudloff
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  4. I. Groeneveld
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  5. J. Gramenz
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  6. C. C. Chu
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Correspondence to Y. P. Wang.

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Wang, Y.P., Sonntag, K., Rudloff, E. et al. Production and characterization of somatic hybrids between Brassica napus and Raphanus sativus . Plant Cell Tiss Organ Cult 86, 279–283 (2006). https://doi.org/10.1007/s11240-006-9118-y

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  • DOI: https://doi.org/10.1007/s11240-006-9118-y

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