Abstract
Shoot clump cultures of Narcissus cultivars St. Keverne and Hawera were used to investigate the effects of culture medium carbon supply, type of carbohydrate and osmolarity on in vitro bulblet development. Increasing the medium osmolarity using mannitol or sorbitol, which did not act as substrates for growth, failed to stimulate bulblet formation with either cultivar. An exception to this was a relatively small increase in total bulblet dry weight per culture, in the cultivar Hawera only, caused by adding 30 g l −1 sorbitol in combination with 30 g l−1 sucrose. Simultaneously increasing the medium osmolarity and carbon supply using the metabolisable carbohydrate sources, sucrose, glucose, fructose or an equimolar mixture of glucose and fructose stimulated bulblet production, total dry matter accumulation and partitioning into bulblets. At comparable levels of carbon supply up to 19.0 g l−1, bulblet development of both cultivars was similar with monosaccharide and sucrose media. This indicates that substrate supply is more important for bulblet development than osmolarity of the culture medium. The cultivar Hawera also showed similar responses to monosaccharide and sucrose media supplying 37.9 g C l−1, despite the high osmolarity of monosaccharide media (c. 650 m Osm kg−1, equivalent to −1.6 MPa, compared to 380 m Osm kg−1 for sucrose medium). However in St. Keverne total dry matter accumulation and dry weight per bulblet were further stimulated only by increasing the sucrose supply from 19.0 to 37.9 g C l−1, not by increasing the monosaccharide supply. Implications of the findings for Narcissus micropropagation are discussed.
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References
J Aartrijk Particlevan PEC der Linde Particlevan (1986) In vitro production of flower bulb crops RH Zimmerman RJ Griesbach FA Hamerschlag BRH Lawson (Eds) Tissue Culture as a Plant Propagation System for Horticultural Crops Martinus Nijhoff Dordrecht 317–331
P Aguettaz A Paffer I Delvallée P der Linde Particlevan GJ de Klerk (1990) ArticleTitleThe development of dormancy in bulbets of Lilium speciosum generated in vitro. 1. The effects of culture conditions Plant Cell Tiss. Org. Cult. 22 167–172
S Chen (1969) ArticleTitleCarbohydrate metabolism in the Narcissus leaf J. Exp. Bot. 20 302–316
YN Chow C Selby BMR Harvey (1992a) ArticleTitleStimulation by sucrose of Naricssus bulblet formation in vitro J. Horticult. Sci. 67 289–293
YN Chow C Selby BMR Harvey (1992b) ArticleTitleA simple method␣for maintaining high multiplication of Narcissus shoot clump cultures in vitro Plant Cell Tiss. Org. Cult. 30 227–230
J Gougler-Smalstig DJ Cosgrove (1987) Solute supply and stem elongation: effect of cotyledon removal on growth, solute and turgor pressure DJ Cosgrove DP Knieval (Eds) Physiology of Cell Expansion during Plant Growth American Society of Plant Physiology Rockville 252–254
GR Hanks (1993) Narcissus M Le Nard AA De Hertogh (Eds) The Physiology of Flower Bulbs Elsevier Amsterdam 463–558
G Hussey NJ Stacey (1984) ArticleTitleFactors affecting the formation of in vitro tubers of potato (Solanum tuberosum L.) Ann. Bot. 53 565–578
M Jefferson-Brown (1991) Narcissus Timberland Press Portland
S Khuri J Moorby (1995) ArticleTitleInvestigations into the role of sucrose in potato cv. Estima microtuber production in vitro Ann. Bot. 75 295–303
S Kington (1989) The International Daffodil Checklist Royal Horticultural Society London
KE Koch (1996) ArticleTitleCarbohydrate regulated gene expression in plants Ann. Rev. Plant Physiol. Plant Mol. Biol. 47 509–540
Lawes Agricultural Trust (1984) Genstat IV. Rothamsted Experimental Station, Harpenden, UK
AR Rees (1972) The Growth of Bulbs Academic Press London
A Santos F Fidalgo I Santos R Salema (2002) ArticleTitleIn vitro bulb formation of Narcissus asturiensis, a threatened species of the Amaryllidaceae J. Horticult. Sci. Biotech. 77 149–152
J Santos I Santos R Salema (1998) ArticleTitleIn vitro production of bulbs of Narcissus bulbocodium flowering in the first season of growth Scientia Horicult. 76 205–217
JEA Seabrook (1990) Narcissus (Daffodil) PV Ammirato DA Evans WR Sharp YPS Bajaj (Eds) Handbook of Plant Cell Culture McGraw-Hill New York 577–597
WM Squires FA Langton (1990) ArticleTitlePotential and limitations of Narcissus micropropagation: an experimental evaluation Acta Horticult. 266 67–75
WM Squires FA Langton JS Fenlon (1991) ArticleTitleFactors influencing transplantation success of micropropagated Naricssus bulblets J. Horticult. Sci. 66 661–671
I Staikidou C Selby BMR Harvey (1994) ArticleTitleStimulation by auxin and sucrose of bulblet formation in vitro by single leaf cultures of Narcissus New Phytol. 127 315–320
S Takayama M Misawa Y Takashige H Tsumori K Ohkawa (1982) ArticleTitleCultivation of in vitro - propagated Lilium bulbs in soil J. Am. Soc. Horticult. Sci. 107 830–834
MR Thompson TJ Douglas H Obata-Sasamoto TA Thorpe (1986) ArticleTitleMannitol metabolism in cultured plant cells Physiol. Plant. 67 365–369
P Trip G Krotkov CD Nelson (1964) ArticleTitleMetabolism of mannitol in higher plants Am. J. Bot. 51 828–835
DA. Webb (1980) Narcissus L TG Tutin VH Heywood NA Burges DM Moore DH Valentine SM Walters DA Webb (Eds) Flora Europea Cambridge University Press Cambridge 78–84
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Staikidou, I., Watson, S., Harvey, B.M.R. et al. Narcissus bulblet formation in vitro: effects of carbohydrate type and osmolarity of the culture medium. Plant Cell Tiss Organ Cult 80, 313–320 (2005). https://doi.org/10.1007/s11240-004-1366-0
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DOI: https://doi.org/10.1007/s11240-004-1366-0