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Salicylic acid induces the change in the adventitious root of Glycyrrhiza uralensis Fisch.: bioactive compounds and antioxidant enzymes

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Abstract

In this study, adventitious roots of Glycyrrhiza uralensis were cultured in a 5-L balloon-type bubble bioreactor with different medium salt strength and different concentrations of sucrose. The results of the culture showed that the best culture conditions were 4 % sucrose concentration and 1 MS medium for the accumulation of secondary metabolites. Salicylic acid has previously been used as an elicitor in tissue cultures to enhance production of secondary metabolites. Addition of 1 mg L−1 salicylic acid significantly enhanced the contents of glycyrrhizic acid (0.31 mg g−1), glycyrrhetinic acid (0.14 mg g−1) and polysaccharide (159.29 mg g−1) in the G. uralensis adventitious roots and the contents were 2.58-fold, 1.27-fold, and 2.07-fold over the control group. Furthermore, the greatest content of total flavonoid (9.40 mg g−1) was observed with 2 mg L−1 salicylic acid added in the culture, which was 2.68-fold higher than the control. The ESI-MSn analysis was also performed, showing that two new kinds of flavonoids, including (3R)-vestitol and glycyrol, were only identified in the salicylic acid treatment. In addition, we also found that salicylic acid significantly increased superoxide dismutases, catalases, and peroxidase activity.

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Acknowledgments

This research was funded by 863 Program (2014AA022201-04), Central Significant Increase or Decrease Program, China (2060302) and National Science and Technology Support Program (2012BAI29B02).

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Authors and Affiliations

  1. Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

    Jing Li, Juan Wang, Jinxin Li & Wenyuan Gao

  2. State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Beijing, 100700, People’s Republic of China

    Jing Li

  3. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Jianli Li & Shujie Liu

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  1. Jing Li
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  2. Juan Wang
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Correspondence to Wenyuan Gao.

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Juan Wang: co-first author.

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Li, J., Wang, J., Li, J. et al. Salicylic acid induces the change in the adventitious root of Glycyrrhiza uralensis Fisch.: bioactive compounds and antioxidant enzymes. Res Chem Intermed 42, 1503–1519 (2016). https://doi.org/10.1007/s11164-015-2099-x

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  • DOI: https://doi.org/10.1007/s11164-015-2099-x

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