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Elicitor-induced accumulation of stilbenes in cell suspension cultures of Cayratia trifolia (L.) Domin

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Abstract

Cell cultures of Cayratia trifolia (Vitaceae), a tropical lianas, were maintained in Murashige and Skoog’s medium containing 0.25 mg l−1 NAA, 0.2 mg l−1 kinetin and casein hydrolysate 250 mg l−1. Cell suspension cultures of C. trifolia accumulate stilbenes (piceid, resveratrol, viniferin, ampelopsin), which on elicitation by any of 500 μM salicylic acid, 100 μM methyl jasmonate, 500 μM ethrel and 500 mg l−1 yeast extract, added on the 7th day, were enhanced by 3- to 6-fold (5–11 mg l−1) by the 15th day.

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Acknowledgments

This work was supported by financial assistance from UGC-DRS under special assistance programme for medicinal plant research and DST-FIST programme for infrastructure development to KGR. The authors wish to thank Prof J.M. Merillon, Faculty Pharmacy, University of Bordeaux-2, France, for the gift of authentic compounds and his help in the work.

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  1. Laboratory of Bio-Molecular Technology, Department of Botany, M. L. Sukhadia University, Udaipur, 313001, India

    Chetana Roat & K. G. Ramawat

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  1. Chetana Roat
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  2. K. G. Ramawat
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Correspondence to K. G. Ramawat.

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Roat, C., Ramawat, K.G. Elicitor-induced accumulation of stilbenes in cell suspension cultures of Cayratia trifolia (L.) Domin. Plant Biotechnol Rep 3, 135–138 (2009). https://doi.org/10.1007/s11816-009-0082-y

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  • DOI: https://doi.org/10.1007/s11816-009-0082-y

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