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Polysaccharide isolated from Glycyrrhiza uralensis Fisch induces intracellular enzyme activity of macrophages

  • Original Article
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Mediterranean Journal of Nutrition and Metabolism

Abstract

A purified fraction of water-soluble polysaccharides was isolated from Glycyrrhiza uralensis Fisch using ion exchange and size exclusion chromatography. The present study was undertaken to discuss the preliminary immunoregulation mechanism of glycyrrhiza polysaccharide (GP) by cytochemistry and quantitative analyses, and intracellular enzyme measurement of macrophages. Acid phosphatase (ACPase), adenosine triphosphatase (ATPase), acid α-naphthyl acetate esterase (ANAE) and succinate dehydrogenase (SDH) in macrophages were stained with different methods. The results indicate that GP increased the production of ACPase, ATPase, ANAE and SDH; the activities of lysozyme (LSZ) and superoxide dismutase (SOD) of macrophages were also induced by GP. Our data suggest that the beneficial therapeutic effects of GP may be attributed partly to its ability to modulate macrophage immune functions.

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References

  1. Jeon YJ, Han SB, Ahn KS et al (1999) Activation of NF-kappaB/Rel in angelan-stimulated macrophages. Immunopharmacology 43:1–9

    Article  CAS  Google Scholar 

  2. Lee KY, Jeon YJ (2003) Polysaccharide isolated from Poria cocos sclerotium induces NF-kappaB/rel activation and iNOS expression in murine macrophages. Int Immunopharmacol 3:1353–1362

    Article  CAS  Google Scholar 

  3. Tzianabos AO (2000) Polysaccharide immunomodulators as therapeutic agents: structural aspects and biologic function. Clin Microbiol Rev 13:523–533

    Article  CAS  Google Scholar 

  4. Lee KY, Jeon YJ (2005) Macrophage activation by polysaccharide isolated from Astragalus membranaceus. Int Immunopharmacol 5:1225–1233.

    Article  CAS  Google Scholar 

  5. Sheela ML, Ramakrishna MK, Bharathi P (2006) Angiogenic and proliferative effects of the cytokine VEGF in Ehrlich ascites tumor cells is inhibited by Glycyrrhiza glabra. Inter Immunopharmacol 6:494–498

    Article  CAS  Google Scholar 

  6. Davis E, Morris D (1991) Medicinal uses of licorice through the millennia: the good and plenty of it. Mol Cell Endocrinol 78:1–6

    Article  CAS  Google Scholar 

  7. Takahara T, Watanabe A (1994) Effects of glycyrrhizin on hepatitis B surface antigen: a biochemical and morphological study. J Hepatol 21:601–609

    Article  CAS  Google Scholar 

  8. Arase Y, Ikeda K, Murashima N et al (1997) The long-term efficacy of glycyrrhizin in chronic hepatitis C patients. Cancer 79:1494–1500

    Article  CAS  Google Scholar 

  9. Yang G, Yu Y (1990) Immunopotentiating effect of traditional Chinese drugs — ginsenoside and glycyrrhiza polysaccharide. Proc Chin Acad Med Sci Peking Union Med Coll 5:188–193

    CAS  Google Scholar 

  10. Nose M, Terawaki K, Oguri K et al (1998) Activation of macrophages by crude polysaccharide fractions obtained from shoots of Glycyrrhiza glabra and hairy roots of Glycyrrhiza uralensis in vitro. Biol Pharm Bull 21:1110–1111

    CAS  Google Scholar 

  11. Takada K, Tomoda M, Shimizu N (1992) Core structure of glycyrrhiza GA, the main polysaccharide from the stolon of Glycyrrhiza glabra var. glandulifera; anti-complementary and alkaline phosphatase-inducing activities of the polysaccharide and its degradation products. Chem Pharm Bull (Tokyo) 40:2487–2490

    CAS  Google Scholar 

  12. Wang YW, Zhang HB, Lv J et al (2000) Inhibition of glycyrrhiza polysaccharides (GPS) on virus. Acta Sci Nat Univ Nankaiensis 33:46–48

    CAS  Google Scholar 

  13. Wang C, Xie GR, Shi YR et al (2003) Study on the anti-tumor effect in vivo of glycyrrhizia polysaccharide and its mechanism. Chin Clin Oncol 8:85–87

    Google Scholar 

  14. Cheng AW, Wan FC, Wang JQ et al (2008) Macrophage immunomodulatory activity of polysaccharides isolated from Glycyrrhiza uralensis Fisch. Int Immunopharmacol 8:43–50

    Article  CAS  Google Scholar 

  15. Taniguchi K, Post RL (1975) Synthesis of adenosine triphosphate and exchange between inorganic phosphate and adenosine triphosphate in sodium and potassium ion transport adenosine triphosphatase. J Biol Chem 250:3010–3018

    CAS  Google Scholar 

  16. Lora PH, Ainsworth JA (2000) Differential cytochemical staining characteristics of channel catfish leukocytes identify cell populations in lymphoid organs. Vet Immunol Immunopathol 73:129–144

    Article  Google Scholar 

  17. Yu LJ, Wang LS, Shao XM et al (2003) Effects of moderate hypothermia on cerebral energy metabolism in neonatal rats with hypoxic-ischemic brain damage. Chin J Contemp Pediatr 5:192–196

    Google Scholar 

  18. Thièrry JP (1967) Mise en evidence des polysaccharides sur coupes fines en microscopie electronique. J Microsc 6:987–1018

    Google Scholar 

  19. Pino RM, Pino LC, Bankston PW (1981) The relationships between the Golgi apparatus. GERL, and lysosomes of fetal rat live Kupffer cells examined by ultrastructural phosphatase cytochemistry. J Histochem Cytochem 29:1061–1070

    CAS  Google Scholar 

  20. Gomori G (1936) Microchemical demonstration of iron. J Pathol 12:655–663

    CAS  Google Scholar 

  21. Inoue T, Iwata H, Matsumura K et al (1988) Acid alpha-naphthyl acetate esterase (ANAE) staining of porcine lymphocytes. Nippon Juigaku Zasshi 50:1169–1176

    CAS  Google Scholar 

  22. Boerjan ML, Baarends WM, Ruven HJ (1991) A cytochemical staining procedure for succinate dehydrogenase activity in preovulatory mouse oocytes embedded in low gelling temperature agarose. J Histochem Cytochem 39:135–138.

    CAS  Google Scholar 

  23. Suzuki I, Tanaka H, Kinoshita A et al (1990) Effect of orally administered β-glucan on macrophage function in mice. Int J Immunopharmacol 12:675–684

    Article  CAS  Google Scholar 

  24. Jeong SC, Jeong YT, Yang BK et al (2006) Chemical characteristics and immuno-stimulating properties of biopolymers extracted from Acanthopanax sessiliflorus. J Biochem Mol Biol 39:84–90

    CAS  Google Scholar 

  25. Catovsky D, Enno A (1977) Morphological and cytochemical identification of lymphoid cells. Lymphology 10:77–84

    CAS  Google Scholar 

  26. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan, 250100, China

    Fachun Wan

  2. Institute of Atomic Energy Application in Agriculture, Shandong Academy of Agricultural Science, 202 Gongyebei Road, Jinan, 250100, China

    Anwei Cheng

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  1. Fachun Wan
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  2. Anwei Cheng
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Correspondence to Anwei Cheng.

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Wan, F., Cheng, A. Polysaccharide isolated from Glycyrrhiza uralensis Fisch induces intracellular enzyme activity of macrophages. Mediterr J Nutr Metab 1, 165–169 (2009). https://doi.org/10.1007/s12349-008-0025-0

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  • DOI: https://doi.org/10.1007/s12349-008-0025-0

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