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Effects of co-overexpression of the genes of Rubisco and transketolase on photosynthesis in rice

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Abstract

Metabolome analyses have indicated an accumulation of sedoheptulose 7-phosphate in transgenic rice plants with overproduction of Rubisco (Suzuki et al. in Plant Cell Environ 35:1369–1379, 2012. doi:10.1111/j.1365-3040.2012.02494.x). Since Rubisco overproduction did not quantitatively enhance photosynthesis even under CO2-limited conditions, it is suspected that such an accumulation of sedoheptulose 7-phosphate hampers the improvement of photosynthetic capacity. In the present study, the gene of transketolase, which is involved in the metabolism of sedoheptulose 7-phosphate, was co-overexpressed with the Rubisco small subunit gene in rice. Rubisco and transketolase were successfully overproduced in comparison with those in wild-type plants by 35–53 and 39–84 %, respectively. These changes in the amounts of the proteins were associated with those of the mRNA levels. However, the rate of CO2 assimilation under high irradiance and different [CO2] did not differ between co-overexpressed plants and wild-type plants. Thus, co-overproduction of Rubisco and transketolase did not improve photosynthesis in rice. Transketolase was probably not a limiting factor of photosynthesis as overproduction of transketolase alone by 80–94 % did not affect photosynthesis.

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Acknowledgments

This study was supported by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (Nos. 26450074 to Y.S. and JP16H02538 to A.M.).

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  1. Yuji Suzuki

    Present address: Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate, 020-8550, Japan

Authors and Affiliations

  1. Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Sendai, 981-8555, Japan

    Yuji Suzuki, Eri Kondo & Amane Makino

  2. CREST, JST, Gobancho, Chiyoda-ku, Tokyo, 102-0076, Japan

    Yuji Suzuki & Amane Makino

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  1. Yuji Suzuki
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Correspondence to Yuji Suzuki.

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Suzuki, Y., Kondo, E. & Makino, A. Effects of co-overexpression of the genes of Rubisco and transketolase on photosynthesis in rice. Photosynth Res 131, 281–289 (2017). https://doi.org/10.1007/s11120-016-0320-4

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