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Overexpression of SBPase enhances photosynthesis against high temperature stress in transgenic rice plants

  • Biotic and Abiotic Stress
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Abstract

Activity of the Calvin cycle enzyme sedoheptulose-1,7-bisphosphatase (SBPase) was increased by overexpression of a rice plants 9,311 (Oryza sativa L.) cDNA in rice plants zhonghua11 (Oryza sativa L.). The genetic engineering enabled the plants to accumulate SBPase in chloroplasts and resulted in enhanced tolerance to high temperature stress during growth of young seedlings. Moreover, CO2 assimilation of transgenic plants was significantly more tolerant to high temperature than that of wild-type plants. The analyses of chlorophyll fluorescence and the content and activation of SBPase indicated that the enhancement of photosynthesis to high temperature was not related to the function of photosystem II but to the content and activation of SBPase. Western blotting analyses showed that high temperature stress led to the association of SBPase with the thylakoid membranes from the stroma fractions. However, such an association was much more pronounced in wild-type plants than that in transgenic plants. The results in this study suggested that under high temperature stress, SBPase maintained the activation of ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) by preventing the sequestration of Rubisco activase to the thylakoid membranes from the soluble stroma fraction and thus enhanced the tolerance of CO2 assimilation to high temperature stress. The results suggested that overexpression of SBPase might be an effective method for enhancing high temperature tolerance of plants.

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Abbreviations

PRK:

Phosphoribulokinase

Rubisco:

Ribulose-1,5-bisphosphate carboxylase–oxygenase

RuBP:

Ribulose-1,5-bisphosphate

Ru5P:

Ribulose-5-bisphosphate

SBP:

Sedoheptulose-1,7-bisphosphate

SBPase:

Sedoheptulose-1,7-bisphosphatase

PSII:

Photosystem II

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Acknowledgments

We are extremely grateful to Li-Zhong Xiong for a gift of the binary vector pU1301 and to Christine A, Raines for a gift of SBPase antibodies, to Henry Miziorko for a gift of PRK antibodies, to Martin Parry for a gift of Rubisco antibodies. This work was supported by the State Key Basic Research and Development Plan of China (No. 2001CB108805), the National Natural Science Foundation of China for Innovative Research Team (No.30521004) and the Program for Changjiang Scholars and Innovative Research Team (PCSIRT).

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Authors and Affiliations

  1. Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Lingling Feng, Kun Wang, Yang Li, Yanping Tan, Jin Kong, Hui Li, Yangsheng Li & Yingguo Zhu

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  1. Lingling Feng
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  2. Kun Wang
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  3. Yang Li
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  5. Jin Kong
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  6. Hui Li
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  7. Yangsheng Li
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  8. Yingguo Zhu
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Correspondence to Yangsheng Li.

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Communicated by A. Atanassov.

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Feng, L., Wang, K., Li, Y. et al. Overexpression of SBPase enhances photosynthesis against high temperature stress in transgenic rice plants. Plant Cell Rep 26, 1635–1646 (2007). https://doi.org/10.1007/s00299-006-0299-y

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  • DOI: https://doi.org/10.1007/s00299-006-0299-y

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