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Characterization of Molecular and Physiological Responses Under Water Deficit of Genetically Modified Soybean Plants Overexpressing the AtAREB1 Transcription Factor

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Abstract

Drought is one of the major factors limiting crop productivity worldwide. Currently, the techniques of genetic engineering are powerful tools for the development of drought-tolerant plants, once they allow for the modification of expression patterns of genes responsive to drought. Within this context, transcription factors recognize specific DNA sequences in the regulatory region of target genes, and thereby regulate their expression. AREB is a transcription factor in the basic leucine zipper family, which binds to the ABRE element in the promoter region of genes induced by abscisic acid and drought. In this study, soybean plants transformed with the 35S:AtAREB1 construct were submitted to drought under greenhouse conditions. AtAREB1 expression was observed in the transgenic lines 1Ea2939 and 1Eb2889, but not in the event 1Ea15 and, under control of the CaMV 35S promoter, did not cause dwarfism and resulted in a higher survival rate of transformed plants after drought and rehydration. Moreover, 1Ea2939 and 1Eb2889 plants presented a greater total number of pods and seeds and increased dry matter content of seeds. The best performance of the transgenic lines 1Ea2939 and 1Eb2889 relative to BR 16 plants (wild type) and to event 1Ea15 might be related to mechanisms of drought prevention through reduced stomatal conductance and leaf transpiration under control conditions. Changes in the expression profile of phosphatases and kinases may also be involved. Such results suggest that the constitutive overexpression of the transcription factor AtAREB1 leads to an improved capacity of the soybean crop to cope with drought with no yield losses.

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Acknowledgments

This study was supported by the Science and Technology Research Partnership for Sustainable Development (SATREPS), Japan International Cooperation Agency (JICA), Japan International Research Center for Agricultural Sciences (JIRCAS), Embrapa Soybean and Londrina State University (UEL). We are also grateful to the Coordination for the Improvement of Higher Education Personnel (CAPES) for granting the scholarship to the authors JP Marinho (Masters student), JFC Carvalho, FA Rodrigues and R Fuganti-Pagliarini (Postdoctoral fellows), the National Council for Scientific and Technological Development (CNPq) for granting the scholarship to the authors LC Ferreira (Postdoctoral fellow) and RA Freitas (PIBIC). The authors are also grateful to staff and students from the laboratories of Agrometeorology and Ecophysiology of Embrapa Soybean for their support with the physiological evaluations. This paper was approved for publication by the Editorial Board of Embrapa Soybean as manuscript number 28/2014.

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    Authors and Affiliations

    1. Londrina State University—Biological Sciences Center, P.O. Box 10.011, 86057-970, Londrina, PR, Brazil

      Juliane Prela Marinho & Silvana Regina Rockenbach Marin

    2. Embrapa Soybean, Rodovia Carlos João Strass, Distrito de Warta, P.O. Box 231, 86001-970, Londrina, PR, Brazil

      Juliane Prela Marinho, Leonardo Cesar Ferreira, Renata Fuganti-Pagliarini, Josirley de Fátima Corrêa Carvalho, Rafaela Alves Freitas, Silvana Regina Rockenbach Marin, Fabiana Aparecida Rodrigues, Liliane Márcia Mertz-Henning, José Renato Bouças Farias, Norman Neumaier, Maria Cristina Neves de Oliveira, Francismar Corrêa Marcelino-Guimarães & Alexandre Lima Nepomuceno

    3. Japan International Research Center for Agricultural Sciences, 1-1 Owashi, Tsukuba, Ibaraki Prefecture, 305-0851, Japan

      Norihito Kanamori, Yasunari Fujita & Kazuo Nakashima

    4. “Luiz Meneghel” Campus, Northern Paraná State University, P.O. Box 261, 86360-000, Bandeirantes, PR, Brazil

      Rafaela Alves Freitas

    5. Laboratory of Plant Molecular Physiology, Tokyo University, 7-3-1 Hongo, Bunkyo, Tokyo, 113-8654, Japan

      Takuya Yoshida & Kazuko Yamaguchi-Shinozaki

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    Corresponding author

    Correspondence to Alexandre Lima Nepomuceno.

    Additional information

    Juliane Prela Marinho and Norihito Kanamori contributed equally to this work.

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    Marinho, J.P., Kanamori, N., Ferreira, L.C. et al. Characterization of Molecular and Physiological Responses Under Water Deficit of Genetically Modified Soybean Plants Overexpressing the AtAREB1 Transcription Factor. Plant Mol Biol Rep 34, 410–426 (2016). https://doi.org/10.1007/s11105-015-0928-0

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