Abstract
Rice cultivation in Northeast India (NEI) primarily relies on rainfed conditions, making it susceptible to severe drought spells that promote the onset of brown spot disease (BSD) caused by Bipolaris oryzae. This study investigates the response of prevalent rice cultivars of NEI to the combined stress of drought and B. oryzae infection. Morphological, physiological, biochemical, and molecular changes were recorded post-stress imposition. Qualitative assessment of reactive oxygen species through DAB (3,3-diaminobenzidine) assay confirmed the elicitation of plant defense responses. Based on drought scoring system and biochemical analyses, the cultivars were categorized into susceptible (Shasharang and Bahadur), moderately susceptible (Gitesh and Ranjit), and moderately tolerant (Kapilee and Mahsuri) groups. Antioxidant enzyme accumulation (catalase, guaiacol peroxidase) and osmolyte (proline) levels increased in all stressed plants, with drought-tolerant cultivars exhibiting higher enzyme activities, indicating stress mitigation efforts. Nevertheless, electrolyte leakage and lipid peroxidation rates increased in all stressed conditions, though variations were observed among stress types. Based on findings from a previous transcriptomic study, a total of nine genes were chosen for quantitative real-time PCR analysis. Among these, OsEBP89 appeared as a potential negative regulatory gene, demonstrating substantial upregulation in the susceptible cultivars at both 48 and 72 h post-treatment (hpt). This finding suggests that OsEBP89 may play a role in conferring drought-induced susceptibility to BSD in the rice cultivars being investigated.
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Acknowledgements
We thank the Director, CSIR-NEIST, Jorhat, for providing us with all the necessary facilities to carry out this work. Authors also acknowledge Dr. Sanjay Kumar Chetia, Chief Scientist of AAU-ARRI, Titabar (Jorhat), for providing the seeds of the cultivars used in this study.
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This work was supported by the Council of Scientific and Industrial Research (CSIR), Govt. of India, as a Focused Basic Research grant (MLP-0007) to CC.
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DD, NC, MS: Methodology, Investigation, Data curation, Writing—original draft, preparation, review and editing, Formal analysis. RS, BS, NV: Data analysis, Critical review & editing of draft manuscript. CC: Conceptualization, Formal analysis, Writing—critical review & editing, Funding acquisition.
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Das, D., Chowdhury, N., Sharma, M. et al. Screening for brown-spot disease and drought stress response and identification of dual-stress responsive genes in rice cultivars of Northeast India. Physiol Mol Biol Plants 30, 647–663 (2024). https://doi.org/10.1007/s12298-024-01447-4
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DOI: https://doi.org/10.1007/s12298-024-01447-4