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Genome-Wide Identification, Expression, and Activity Analysis of Alkaline/Neutral Invertase Gene Family from Cassava (Manihot esculenta Crantz)

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Abstract

Alkaline/neutral invertase comprises a family of enzymes that catalyze the irreversible hydrolysis of sucrose into glucose and fructose. They play a crucial role in carbohydrate partitioning and developmental processes of plants. In this study, we genome-wide identified 11 alkaline/neutral invertase family genes (MeNINVs) from the cassava genome. Based on phylogeny, MeNINVs could be classified into α and β groups. The α group genes are encoded by six exons (MeNINV1, 6, 7, 8, and 10) or four exons (MeNINV9), whereas the β group genes are encoded by four exons (MeNINV2, 3, 4, and nINV1) or five exons (MeNINV5). Their catalytic residues of motifs 2 and 4 for sucrose hydrolysis locate in all MeNINVs. qRT-PCR analysis has demonstrated that all MeNINVs are highly expressed in floral organs. During tuber development, MeNINV1, 6, 10, and nINV1 are strongly expressed in source tissues (leaves), and MeNINV1 and nINV1 also play an important role in sink organs (tuber). Activity analysis of alkaline/neutral invertase and determination of sucrose content in source and sink organs of cassava indicates that lower efficiency of sucrose hydrolysis in source organs (leaves) and higher efficiency in sink organs (tuber) are beneficial sucrose to transport from source to sink organs.

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Abbreviations

a.a:

Amino acids

bp:

Base pair

cDNA:

Complementary DNA

INV:

Invertase

kDa:

Kilodaltons

MW:

Molecular weight

ORF:

Open reading frame

PCR:

Polymerase chain reaction

pI:

Isoelectric point

qRT-PCR:

Real-time quantitative PCR

RT-PCR:

Reverse transcription polymerase chain reaction

SuSy:

Sucrose synthase

UDP-glucose:

Uridine 5′-diphosphoglucose

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Acknowledgments

This study was supported by the National Basic Research Program of China (no. 2010CB126600), the National Natural Science Foundation of China (no. 31170234), and the Major Technology Project of Hainan (no. ZDZX2013023-1). The Fundamental Scientific Research Funds for Chinese Academy of Tropical Agricultural Sciences (No. CATAS-1630052014004).

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Authors and Affiliations

  1. Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

    Yuan Yao, Meng-Ting Geng, Jiao Liu, Rui-Mei Li & Jian-Chun Guo

  2. Agricultural College of Hainan University, Haikou, 570228, China

    Yuan Yao, Xiao-Hui Wu & Xin-Wen Hu

Authors
  1. Yuan Yao
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  2. Meng-Ting Geng
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  3. Xiao-Hui Wu
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  4. Jiao Liu
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  5. Rui-Mei Li
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  6. Xin-Wen Hu
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  7. Jian-Chun Guo
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Correspondence to Xin-Wen Hu or Jian-Chun Guo.

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Yuan Yao and Meng-ting Geng contributed equally to this work.

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Yao, Y., Geng, MT., Wu, XH. et al. Genome-Wide Identification, Expression, and Activity Analysis of Alkaline/Neutral Invertase Gene Family from Cassava (Manihot esculenta Crantz). Plant Mol Biol Rep 33, 304–315 (2015). https://doi.org/10.1007/s11105-014-0743-z

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