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Molecular cloning and expression analysis of the cell-wall invertase gene family in rice (Oryza sativa L.)

  • Genetics and Genomics
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Abstract

Cell-wall invertase (CIN) catalyzes the hydrolysis of sucrose into glucose and fructose for the supply of carbohydrates to sink organs via an apoplastic pathway. To study the CIN genes in rice (Oryza sativa L.), we isolated cDNA clones showing amino acid similarity to the plant cell wall invertase proteins from a search of rice sequence databases. Profile analyses revealed that the cloned genes are expressed in unique patterns in various organs. For example, transcripts of OsCIN1, OsCIN2, OsCIN4, and OsCIN7 were detected in immature seeds whereas OsCIN3 gene expression was flower-specific. Further transcript analysis of these genes expressed in developing seeds indicated that OsCIN1, OsCIN2, and OsCIN7 might play an important role involving sucrose partitioning to the embryo and endosperm. Sucrose, a substrate of CINs, induced the accumulation of OsCIN1 transcripts in excised leaves and OsCIN2 in immature seeds, while the level of OsCIN5 was significantly down-regulated in excised leaves treated with sucrose. Infecting the tissues with rice blast (Magnaporthe grisea) as a biotic stressor increased the expression of OsCIN1, OsCIN4, and OsCIN5, suggesting that these genes may participate in a switch in metabolism to resist pathogen invasion. These results demonstrate that OsCIN genes play diverse roles involving the regulation of metabolism, growth, development, and stress responses.

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Acknowledgements

We thank the members of the Plant Metabolism Research Centre (PMRC) for helpful discussions, Junok Lee for technical assistance, and Priscilla Licht for the critical reading of the manuscript. This work was supported, in part, by grants from SRC for the PMRC, Korea Science and Engineering Foundation (KOSEF) Program; from the Biogreen 21 Program, Rural Development Administration; from the Crop Functional Genomic Centre, the 21 Century Frontier Program; and from the BK21 program, Ministry of Education.

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  1. Plant Metabolism Research Centre & Graduate School of Biotechnology, Kyung Hee University, Suwon, 449-701, Korea

    Jung-Il Cho, Sang-Kyu Lee, Seho Ko, He-Kyung Kim, Youn-Hyung Lee, Seong Hee Bhoo, Tae-Ryong Hahn & Jong-Seong Jeon

  2. Division of Biological Sciences, Seoul National University, Seoul, 151-742, Korea

    Jung-Il Cho & Kwang-Woong Lee

  3. Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, 790-784, Korea

    Sung-Hoon Jun & Gynheung An

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  1. Jung-Il Cho
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Communicated by I.S. Chung

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Cho, JI., Lee, SK., Ko, S. et al. Molecular cloning and expression analysis of the cell-wall invertase gene family in rice (Oryza sativa L.). Plant Cell Rep 24, 225–236 (2005). https://doi.org/10.1007/s00299-004-0910-z

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