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Promoter Analysis for Three Types of EUL-Related Rice Lectins in Transgenic Arabidopsis

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Abstract

In this study, the promoter activity for three types of Euonymus-related lectins (EUL) from rice, further referred to as OrysaEULS2, OrysaEULS3, and OrysaEULD1A was analyzed. In silico promoter analyses showed that the EUL promoters from rice contain next to the typical promoter elements some motifs that are considered to be stress-responsive elements. Furthermore, Arabidopsis thaliana plants were transformed with a promoter::β-glucuronidase (GUS) construct for each of the proteins under study. Subsequently, one-insertion homozygous lines were selected and analyzed for GUS activity. Experiments were performed under normal growth conditions or after application of different stress conditions, in particular treatments with 150 mM NaCl, 100 mM mannitol, and 100 μM abscisic acid (ABA) for 24 h. GUS activity was detected with the OrysaEULS3 and OrysaEULD1A promoters especially in the cotyledons and the young true leaves, respectively, but not with the OrysaEULS2 promoter. The activity of OrysaEULS3 and OrysaEULD1A promoters was increased after ABA and mannitol treatments but decreased after NaCl treatment. We hypothesize that the Euonymus-related rice proteins have a role in sensing and responding to external stresses as well as in the growth of the plant.

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Abbreviations

EUL:

Euonymus lectin

GUS:

β-Glucuronidase

OSR40:

Oryza sativa repeats 40 kDa

TSS:

Transcription start site

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Acknowledgments

This work was funded primarily by the Fund for Scientific Research—Flanders (FWO grant G.0022.08), the Research Council of Ghent University (project BOF10/GOA/003), and the Hercules Foundation. Bassam Al Atalah is a recipient of a doctoral grant from the Special Research Council of Ghent University.

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  1. Department of Molecular Biotechnology, Laboratory of Biochemistry and Glycobiology, Ghent University, Coupure Links 653, 9000, Ghent, Belgium

    Bassam Al Atalah, Elke Fouquaert & Els J. M. Van Damme

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  1. Bassam Al Atalah
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  2. Elke Fouquaert
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  3. Els J. M. Van Damme
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Correspondence to Els J. M. Van Damme.

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Supplementary Fig. 1

Histochemical analysis of GUS activity in Arabidopsis seedlings harboring a 35S promoter::GUS construct. Images were taken using a Nikon eclipse TE2000-e epi-fluorescence Microscope (Nikon Benelux, Brussels, Belgium) and a Leica microscope (Leica, Nussloch, Germany) (PPTX 264 kb)

Supplementary Fig. 2

Histochemical analysis of GUS activity in Arabidopsis seedlings. No GUS staining was detected for Arabidopsis plants harbouring the OrysaEULS2 promoter. Non-treated and stress-treated plant tissues did not show any GUS activity. Some representative pictures are shown for each plant stage (a). GUS staining was absent in the leaf, stem and root samples analyzed for developmental stages 4 and 5 of Arabidopsis plants expressing each of the EUL promoter constructs.. Some representative pictures are shown for Arabidopsis plants harboring the OrysaEULD1A promoter at developmental stage 5 (b). Images were taken using a Nikon eclipse TE2000-e epi-fluorescence Microscope (Nikon Benelux, Brussels, Belgium) and a Leica microscope (Leica, Nussloch, Germany) (PPTX 493 kb)

Supplementary Fig. 3

Comparative analysis of GUS activity under OrysaEULD1A promoter. GUS staining was analyzed in seedlings of plant stage 1 grown on MS (a), or after mannitol (b) and ABA (c) treatments (PPTX 1.50 MB)

Supplementary Fig. 4

Comparative analysis of GUS activity under OrysaEULS3 promoter. GUS staining was analyzed in seedlings of plant stage 2 grown on MS (a), or after mannitol (b) and ABA (c) treatments (PPTX 188 KB)

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Al Atalah, B., Fouquaert, E. & Van Damme, E.J.M. Promoter Analysis for Three Types of EUL-Related Rice Lectins in Transgenic Arabidopsis . Plant Mol Biol Rep 31, 1315–1324 (2013). https://doi.org/10.1007/s11105-013-0611-2

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