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Characterization of promoter activity of the ethylene receptor gene OgERS1 from Oncidesa in transgenic Arabidopsis

  • Original papers
  • Published:
Biologia Plantarum

Abstract

Physiological changes associated with senescence of flowers and abscission of floral parts in Oncidesa (formerly Oncidium) cv. Gower Ramsey are caused by a plant hormone ethylene which is produced by pollinia cap dislodgment during postharvest handling and transportation. The ethylene receptor gene OgERS1 of Oncidesa has been previously cloned and characterized. To analyze promoter activity of OgERS1, transgenic Arabidopsis thaliana plants were generated to express the ß-glucuronidase (GUS) reporter gene under the control of 5’-upstream sequence of OgERS1 from Oncidesa. The expression pattern of the OgERS1 promoter at the cellular level was investigated by analysis of GUS activity. This promoter can activate gene expression in both actively dividing young tissues and abscission-related aging tissues. Expression of GUS was detected in the shoot meristem uniquely in 10 to 30 d-old-plants and was found in flower buds, axillary buds, flower stems, and abscission layers during later development. In 2- to 3-week-old transgenic Arabidopsis, exogenous ethylene, glucose, lactose, and maltose enhanced promoter activity implying that crosstalk between sugar and an ethylene receptor may exist. However, indole-3-acetic acid, benzylaminopurine, abscisic acid, heat, wounding, salinity, drought, and flooding slightly suppressed promoter activity. These results demonstrate that the promoter of OgERS1 was developmentally and environmentally regulated, and imply a potential for application of this bi-functional promoter to increase branching or enhanced dwarfing.

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Abbreviations

ABA:

abscisic acid

BA:

6-benzylaminopurine

ETR:

ethylene response

ERS:

ethylene response sensor

GA:

gibberellic acid

GUS:

ß-glucuronidase

IAA:

indole-3-acetic acid

MeJA:

methyl jasmonate

MS:

Murashige and Skoog

OgERS:

Oncidesa ethylene response sensor

SA:

salicylic acid

TBA:

tertiary butyl alcohol

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Authors and Affiliations

  1. Department of Horticulture and Landscape Architecture, National Taiwan University, Taipei, 10617, Taiwan, ROC

    W.-F. Huang, P.-L. Huang & Y.-Y. Do

  2. Graduate Institute of Biotechnology, Chinese Culture University, Taipei, 11114, Taiwan, ROC

    P.-L. Huang

Authors
  1. W.-F. Huang
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  2. P.-L. Huang
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  3. Y.-Y. Do
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Corresponding author

Correspondence to Y.-Y. Do.

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Acknowledgments: We are grateful for proofreading of this manuscript by Dr. Mark D. Barnes (the Chinese Culture University) and Dr. Y.F. Sun. This work was supported by Grants 91AS-3.1.3-FD-Z6(1), 92AS-4.2.1-FD-Z2, and 93AS-4.1.2-FD-Z1 from the Council of Agriculture, Republic of China.

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Huang, WF., Huang, PL. & Do, YY. Characterization of promoter activity of the ethylene receptor gene OgERS1 from Oncidesa in transgenic Arabidopsis . Biol Plant 60, 261–268 (2016). https://doi.org/10.1007/s10535-015-0578-y

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  • DOI: https://doi.org/10.1007/s10535-015-0578-y

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