Skip to main content
SpringerLink
Log in

Proteomic Analysis of PEG-Fractionated UV-C Stress-Response Proteins in Globe Artichoke

  • Published:
Plant Molecular Biology Reporter Aims and scope Submit manuscript

Abstract

Plants respond to UV stress by producing antioxidant molecules and by altering their metabolism through the regulation of specific gene family members. Globe artichoke (Cynara cardunculus var. scolymus L.—Compositae family) is an attractive model species for studying the protein networks involved in UV response, being characterised by high loads of antioxidants, which are boosted by UV-C rays. The effect of UV-C on leaf proteome was investigated 24 h after irradiation by applying a polyethylene glycol (PEG) fractionation method, which we previously developed to cope with the so-called high abundance proteins, followed by two-dimensional electrophoresis analysis. Thirty-eight UV-C modulated proteins were analysed by means of a nano-liquid chromatography–nanospray tandem mass spectrometry, and 27 were successfully identified using a customised Compositae protein database, including a set of 19,055 globe artichoke unigenes. Proteins were categorised according to Gene Ontology (GO) and visualised on their cellular pathway: Most of them were involved not only in primary metabolism (e.g. photosynthesis) and in abiotic stress responses (e.g. reactive oxygen species scavenging) but also in cell wall modification, secondary metabolism, and regulation of transcription. To date, this is the first report on globe artichoke proteomic expression analysis following abiotic stress. Our results confirm the effectiveness of the PEG fractionation method in excluding most of the ribulose bisphosphate carboxylase/oxygenase and highlighting some major occurring phenomena.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Acquadro A, Falvo S, Mila S, Albo A, Comino C, Moglia A, Lanteri S (2009) Proteomics in globe artichoke: protein extraction and sample complexity reduction by PEG fractionation. Electrophoresis 30(9):1594–1602

    Article  PubMed  CAS  Google Scholar 

  • Ahsan N, Lee D, Alam I et al (2008) Comparative proteomic study of arsenic-induced differentially expressed proteins in rice roots reveals glutathione plays a central role during As stress. Proteomics 8:3561–3576

    Google Scholar 

  • Albo A, Mila S, Digilio G et al (2007) Proteomic analysis of a genetically modified maize flour carrying Cry1Ab gene and comparison to the corresponding wild-type. Maydica 52:443–455

    Google Scholar 

  • Bian F, Zheng C, Qu F, Gong X, You C (2010) Proteomic analysis of somatic embryogenesis in Cyclamen persicum Mill. Plant Mol Biol Report 28(1):22–31

    Article  CAS  Google Scholar 

  • Bollenbach T, Sharwood R, Gutierrez R et al (2009) The RNA-binding proteins CSP41a and CSP41b may regulate transcription and translation of chloroplast-encoded RNAs in Arabidopsis. Plant Mol Biol 69:541–552

    Google Scholar 

  • Braam J (1992) Regulation of expression of calmodulin and calmodulin-related genes by environmental stimuli in plants. Cell Calcium 13:457–463

    Google Scholar 

  • Braam J, Davis R (1990) Rain-induced, wind-induced, and touch-induced expression of calmodulin and calmodulin-related genes in Arabidopsis. Cell 60:357–364

    Google Scholar 

  • Bradford M (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  • Casati P, Zhang X, Burlingame AL, Walbot V (2005) Analysis of leaf proteome after UV-B irradiation in maize lines differing in sensitivity. Mol Cell Proteomics 4:1673–1685

    Article  PubMed  CAS  Google Scholar 

  • Cho S, Kim J, Park J et al (2006) Constitutive expression of abiotic stress-inducible hot pepper CaXTH3, which encodes a xyloglucan endotransglucosylase/hydrolase homolog, improves drought and salt tolerance in transgenic Arabidopsis plants. FEBS Lett 580:3136–3144

    Google Scholar 

  • Conesa A, Gotz S, Garcia-Gomez J, Terol J, Talon M, Robles M (2005) Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics 21(18):3674–3676

    Article  PubMed  CAS  Google Scholar 

  • Coohill T (1999) Conventional UV-visible sources. Photochem Photobiol 69:15S

    Google Scholar 

  • Damerval C, De Vienne D, Zivy MHT (1986) Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat-seedling proteins. Electrophoresis 7:52–54

    Article  CAS  Google Scholar 

  • Ding C, You J, Liu Z, Rehmani MIA, Wang S, Li G, Wang Q, Ding Y (2011) Proteomic analysis of low nitrogen stress-responsive proteins in roots of rice. Plant Mol Biol Report. doi:10.1007/s11105-010-0268-z

    Google Scholar 

  • Dong M, Zhang X, Zhuang Z, Zou J, Ye N, Xu D, Mou S, Liang C, Wang W (2011) Characterization of the LhcSR gene under light and temperature stress in the green alga Ulva linza. Plant Mol Biol Report. doi:10.1007/s11105-011-0311-8

    Google Scholar 

  • Du HM, Liang Y, Pei KQ, Ma K (2011) UV radiation-responsive proteins in rice leaves: a proteomic analysis. Plant Cell Physiol 52(2):306–316

    Article  PubMed  CAS  Google Scholar 

  • Feng L, Wang K, Li Y et al (2007) Overexpression of SBPase enhances photosynthesis against high temperature stress in transgenic rice plants. Plant Cell Rep 26:1635–1646

    Google Scholar 

  • Iliev E, Xu W, Polisensky D et al (2002) Transcriptional and posttranscriptional regulation of Arabidopsis TCH4 expression by diverse stimuli. Roles of cis regions and brassinosteroids. Plant Physiol 130:770–783

    Google Scholar 

  • Jellouli N, Jouira HB, Daldoul S, Chenennaoui S, Ghorbel A, Salem AB, Gargouri A (2010) Proteomic and transcriptomic analysis of grapevine pr10 expression during salt stress and functional characterization in yeast. Plant Mol Biol Report 28(1):1–8

    Article  CAS  Google Scholar 

  • Kaspar S, Matros A, Mock HP (2010) Proteome and flavonoid analysis reveals distinct responses of epidermal tissue and whole leaves upon UV−B Radiation of barley (Hordeum vulgare L.) seedlings. J Proteome Res 9(5):2402–2411

    Article  PubMed  CAS  Google Scholar 

  • Kasprzewska A (2003) Plant chitinases—regulation and function. Cell Mol Biol Lett 8:809–824

    Google Scholar 

  • Kun W, Feng G, Renshan Z, Shaoqing L, Yingguo Z (2011) Expression, purification, and secondary structure prediction of pentatricopeptide repeat protein RF1A from rice). Plant Mol Biol Report. doi:10.1007/s11105-010-0260-7

    Google Scholar 

  • Kuun K, Okole B, Bornman L (2001) Protection of phenylpropanoid metabolism by prior heat treatment in Lycopersicon esculentum exposed to Ralstonia solanacearum. Plant Physiol Biochem 39:871–880

    Google Scholar 

  • Lefebvre S, Lawson T, Zakhleniuk O et al (2005) Increased sedoheptulose-1,7-bisphosphatase activity in transgenic tobacco plants stimulates photosynthesis and growth from an early stage in development. Plant Physiol 138:451–460

    Google Scholar 

  • Li D, Staehelin C, Wang W, Peng S (2010) Molecular cloning and characterization of a chitinase-homologous gene from Mikania micrantha infected by Cuscuta campestris. Plant Mol Biol Report 28(1):90–101

    Article  CAS  Google Scholar 

  • Michiels A, Van Laere A, Van den Ende W et al (2004) Expression analysis of a chicory fructan 1-exohydrolase gene reveals complex regulation by cold. J Exp Bot 55:1325–1333

    Google Scholar 

  • Miyagawa Y, Tamoi M, Shigeoka S (2001) Overexpression of a cyanobacterial fructose-1,6-/sedoheptulose-1,7-bisphosphatase in tobacco enhances photosynthesis and growth. Nat Biotechnol 19:965–969

    Google Scholar 

  • Moglia A, Lanteri S, Comino C, Acquadro A, de Vos R, Beekwilder J (2008) Stress-induced biosynthesis of dicaffeoylquinic acids in globe artichoke. J Agric Food Chem 56(18):8641–8649

    Article  PubMed  CAS  Google Scholar 

  • Moon Y, Wang X, Morris M (2006) Dietary flavonoids: effects on xenobiotic and carcinogen metabolism. Toxicol in Vitro 20:187–210

    Google Scholar 

  • Petit A, Baillieul F, Vaillant-Gaveau N et al (2009) Low responsiveness of grapevine flowers and berries at fruit set to UV-C irradiation. J Exp Bot 60:1155–1162

    Google Scholar 

  • Polisensky D, Braam J (1996) Cold-shock regulation of the arabidopsis TCH genes and the effects of modulating intracellular calcium levels. Plant Physiol 11:1271–1279

    Google Scholar 

  • Qian L, Wenyu Y, Xiaoping Y, Songlin R, Haifeng C, Huasheng M, Zihong Y (2011) A preliminary study on proteome variations associated with gall formation in Zizania latifolia Trucs). Plant Mol Biol Report 29(2):360–368

    Article  Google Scholar 

  • Ramnani PGE, Bingham M, van Bruggen P, Tuohy KM, Gibson GR (2010) Prebiotic effect of fruit and vegetable shots containing Jerusalem artichoke inulin: a human intervention study. Br J Nutr 1:1–8

    Google Scholar 

  • Regalado A, Pinheiro C, Vidal S et al (2000) The Lupinus albus class-III chitinase gene, IF3, is constitutively expressed in vegetative organs and developing seeds. Planta 210:543–550

    Google Scholar 

  • Righetti P, Boschetti E (2007) Sherlock Holmes and the proteome—a detective story. FEBS J 274(4):897–905

    Article  PubMed  CAS  Google Scholar 

  • Ronkart S, Blecker C, Fourmanoir H et al (2007) Isolation and identification of inulooligosaccharides resulting from inulin hydrolysis. Anal Chim Acta 604:81–87

    Google Scholar 

  • Scaglione D, Acquadro A, Portis E, Taylor CA, Lanteri S, Knapp SJ (2009) Ontology and diversity of transcript-associated microsatellites mined from a globe artichoke EST database. BMC Genomics 10(1):454

    Article  PubMed  Google Scholar 

  • Shevchenko A, Tomas H, Havlis J et al (2006) In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nat Protoc 1:2856–2860

    Google Scholar 

  • Singh A, Sarkar A, Singh S et al (2010) Investigation of supplemental ultraviolet-B-induced changes in antioxidative defense system and leaf proteome in radish (Raphanus sativus L. cv Truthful): an insight to plant response under high oxidative stress. Protoplasma 245:75–83

    Google Scholar 

  • Stapletons A (1992) Ultraviolet-radiation and plants—burning questions. Plant Cell 4:1353–1358

    Google Scholar 

  • Timperio A, Egidi M, Zolla L (2008) Proteomics applied on plant abiotic stresses: role of heat shock proteins (HSP). J Proteomics 71:391–411

    Google Scholar 

  • Van den Ende W, De Coninck B, Van Laere A (2004) Plant fructan exohydrolases: a role in signaling and defense? Trends Plant Sci 9:523–528

    Google Scholar 

  • Wang M, Simon J, Aviles I, He K, Zheng Q, Tadmor Y (2003) Analysis of antioxidative phenolic compounds in artichoke (Cynara scolymus L.). J Agric Food Chem 51(3):601–608

    Article  PubMed  CAS  Google Scholar 

  • Wang W, Vinocur B, Shoseyov O et al (2004) Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends Plant Sci 9:244–252

    Google Scholar 

  • Winkel B (2004) Metabolic channeling in plants. Annu Rev Plant Biol 55:85–107

    Google Scholar 

  • Xu C, Sullivan JH, Garrett WM, Caperna TJ, Natarajan S (2008) Impact of solar ultraviolet-B on the proteome in soybean lines differing in flavonoid contents. Phytochemistry 69(1):38–48

    Article  PubMed  CAS  Google Scholar 

  • Yamamori A, Onodera S, Kikuchi M et al (2002) Two novel oligosaccharides formed by 1(F)-fructosyltransferase purified from roots of asparagus (Asparagus officinalis L.). Biosci Biotechnol Biochem 66:1419–1422

    Google Scholar 

  • Yang Q, Wang Y, Zhang J et al (2007) Identification of aluminum-responsive proteins in rice roots by a proteomic approach: cysteine synthase as a key player in Al response. Proteomics 7:737–749

    Google Scholar 

  • Zhang J, Sun L, Liu L, Lian J, An S, Wang X, Zhang J, Jin J, Li S, Xi J (2010) Proteomic analysis of interactions between the generalist herbivore Spodoptera exigua (Lepidoptera: Noctuidae) and Arabidopsis thaliana. Plant Mol Biol Report 28(2):324–333

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research has been enabled by a grant from “Progetto Lagrange-Fondazione CRT”. The research was performed in the context of the project iTECHPLAT Intervention, co-financed by the EU on the Measure 3.4 of the Regione Piemonte docup 2000/2006, supported and implemented by Polo di Innovazione Provinciale of the Province of Turin 2009. Special thanks are due to Franco Fedeli for his training on HPLC analysis.

Author information

Authors and Affiliations

  1. DiVaPRA, University of Turin, via L. da Vinci 44, 10095, Grugliasco, Turin, Italy

    Sara Falvo, Alberto Acquadro & Sergio Lanteri

  2. LIMA, Bioindustry Park Silvano Fumero, via Ribes 5, 10010, Colleretto Giacosa, Turin, Italy

    Sara Falvo & Alessandra Giuliano Albo

  3. Plant Research International, Droevendaalsesteeg 1B, 6708PB, Wageningen, The Netherlands

    Twan America

Authors
  1. Sara Falvo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alberto Acquadro
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alessandra Giuliano Albo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Twan America
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sergio Lanteri
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Alberto Acquadro.

Electronic Supplementary Materials

Below is the link to the electronic supplementary material.

Supplementary Material 1

Bar diagram of the UV-C modulated proteins with fold change and standard error. (PPT 232 kb)

Supplementary Material 2

Protein identification from the globe artichoke PEG-enriched proteome 24 h after UV-C irradiation with peptides. (DOC 637 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Falvo, S., Acquadro, A., Albo, A.G. et al. Proteomic Analysis of PEG-Fractionated UV-C Stress-Response Proteins in Globe Artichoke. Plant Mol Biol Rep 30, 111–122 (2012). https://doi.org/10.1007/s11105-011-0325-2

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11105-011-0325-2

Keywords

Search

Navigation