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A Preliminary Study on Proteome Variations Associated with Gall Formation in Zizania latifolia Trucs

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Abstract

Zizania latifolia Trucs is a uniquely flavored aquatic vegetable found in southern and eastern Asia. Several physiology and genetic approaches have been employed to increase our knowledge about the physiological basis of gall formation; however, as yet, data at the proteomic level are not available. Protein yield and sodium dodecyl sulfate-polyacrylamide gel electrophoresis were used to determine the most appropriate protein extraction methods for use in this study. Total proteins were extracted from the culm tissue at three relevant developmental stages and separated by two-dimensional gel electrophoresis. The number and abundance of spots varied among the two-dimensional gel electrophoresis gels at the three stages. Proteins with more than 1.7-fold abundance between the different stages were monitored. We identified 10 well-resolved spots by matrix-assisted laser-desorption ionization/time-of-flight peptide mass fingerprinting and tandem mass spectrometry. Some spots linked to signal transduction of the phytohormone could be identified. The expression volume of these spots transiently increased during the expansion phase. In contrast, the spots linked to phytoene dehydrogenase and methionine synthase or pyrophosphate-dependent phosphofructokinase were more abundant during gall formation, showing an increase in spot intensity during development and maximal abundance in mature gall. Higher intensity was also found in the spots linked to stress response. We discuss protein variations, considering their potential role during gall formation and comparing our results with established variations at metabolite-profiling levels.

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Acknowledgement

The authors would like to thank the editor and anonymous referees for their helpful comments and suggestions that led to this revised version. This work was supported in part by the “National Natural Science Foundation of China (30940018),” the Zhejiang Province Important Science and Technology Tackled Project (2004C22003), and the Natural Science Foundation of Zhejiang Province, China (Y3090173).

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Authors and Affiliations

  1. College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang, 310018, China

    Liu Qian, You Wenyu, Yu Xiaoping, Cui Haifeng & Ye Zihong

  2. Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, Hangzhou, Zhejiang, 310018, China

    Liu Qian, You Wenyu, Yu Xiaoping, Cui Haifeng & Ye Zihong

  3. Plant Molecular Biology & Proteomics Lab, Institute of Biotechnology Hangzhou Academy of Agriculture Science, Hangzhou, Zhejiang, 310024, China

    Ruan Songlin & Ma Huasheng

  4. Department of Biotechnology, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang, China, 310018

    Ye Zihong

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  1. Liu Qian
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Correspondence to Ye Zihong.

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Liu Qian and You Wenyu are co-first author

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Qian, L., Wenyu, Y., Xiaoping, Y. et al. A Preliminary Study on Proteome Variations Associated with Gall Formation in Zizania latifolia Trucs. Plant Mol Biol Rep 29, 360–368 (2011). https://doi.org/10.1007/s11105-010-0236-7

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  • DOI: https://doi.org/10.1007/s11105-010-0236-7

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