Skip to main content
SpringerLink
Log in

Genetic Diversity Among Jatropha and Jatropha-Related Species Based on ISSR Markers

  • Published:
Plant Molecular Biology Reporter Aims and scope Submit manuscript

Abstract

Jatropha curcas (jatropha) is a potential biodiesel crop. A major limitation in production is that jatropha remains wild with low genetic variation. Related species/genera in the Euphorbiaceae can potentially be used for its genetic improvement. In this study, we employed inter-simple sequence repeats (ISSRs) to assess genetic variation among 30 accessions of jatropha, two accessions of bellyache bush (Jatropha gossypifolia), two accessions of spicy jatropha (Jatropha integerrima), two accessions of bottleplant shrub (Jatropha podagrica), and three accessions of castor bean hybrids. Genetic relationships were evaluated using 27 of 86 ISSR markers, yielding 307 polymorphic bands with polymorphism contents ranging from 0.76 to 0.95 for IMPN 1 and UBC 807 markers, respectively. Dice’s genetic similarity coefficient ranged from 0.39 to 0.99, which clearly separated the plant samples into seven groups at the coefficient of 0.48. The first group comprised J. curcas from Mexico, the second group comprised J. curcas from China and Vietnam, the third group comprised J. curcas from Thailand, the fourth group was J. integerrima, the fifth group was J. gossypifolia, the sixth group was J. podagrica, and the last and most distinct group was Ricinus communis. Analysis of molecular variance revealed that 63% of the variability was attributable to variation among groups, while 37% was due to variation within groups. Based on Nei’s genetic distance, the population from G2 (J. curcas from China) and G4 (J. curcas from Vietnam) had the least ISSR variability (0.0668), whereas G8 (R. communis) and Jatropha spp. displayed the highest distance (0.6005–0.7211).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Basha SD, Sujatha M (2007) Inter and intra-population variability of Jatropha curcas (L.) characterized by RAPD and ISSR markers and development of population-specific SCAR markers. Euphytica 156(3):375–386

    Article  CAS  Google Scholar 

  • Basha SD, Sujatha M (2009) Genetic analysis of Jatropha species and interspecific hybrids of Jatropha curcas using nuclear and organelle specific markers. Euphytica 168(2):197–214

    Article  CAS  Google Scholar 

  • Basha SD, Francis G, Makkar HPS, Becker K, Sujatha M (2009) A comparative study of biochemical traits and molecular markers for assessment of genetic relationships between Jatropha curcas L. germplasm from different countries. Plant Sci 176:812–823

    Article  CAS  Google Scholar 

  • Bhagyawant SS, Srivastava N (2008) Genetic fingerprinting of chickpea (Cicer arietinum L.) germplasm using ISSR markers and their relationships. Afr J Biotechnol 7(24):4428–4431

    CAS  Google Scholar 

  • Blair MW, Panaud O, McCouch SR (1999) Inter-simple sequence repeat (ISSR) amplification for analysis of microsatellite motif frequency and fingerprinting in rice (Oryza sativa L.). Theor Appl Genet 98:780–792

    Article  CAS  Google Scholar 

  • Bostein D, White RL, Sholnick M, David RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphism. Am J Hum Genet 32:314–331

    Google Scholar 

  • Devappa RK, Swamylingappa B (2008) Biochemical and nutritional evaluation of Jatropha protein isolate prepared by steam injection heating for reduction of toxic and antinutritional factors. J Sci Food Agric 88:911–919

    Article  CAS  Google Scholar 

  • Dice LR (1945) Measures of the amount of ecological association between species. Ecology 26:297–302

    Article  Google Scholar 

  • Francis G, Edinger R, Becker K (2005) A concept for simultaneous wasteland reclamation, fuel production, and socio-economic development in degraded areas in India: Need, potential and perspectives of Jatropha plantations. Nat Resour Forum 29:12–24

    Article  Google Scholar 

  • Ganesh Ram S, Parthiban KT, Senthil Kumar R, Thiruvengadam V, Paramathma M (2008) Genetic diversity among Jatropha species as revealed by RAPD markers. Genet Resour Crop Evol 55:803–809

    Article  Google Scholar 

  • Guillemaut P, Maréchal-Drouard L (1992) Isolation of plant DNA: a fast, inexpensive, and reliable method. Plant Mol Biol Report 10(1):60–65

    Article  CAS  Google Scholar 

  • Gupta S, Srivastava M, Mishra GP, Naik PK, Chauhan RS, Tiwari SK, Kumar M, Singh R (2008) Analogy of ISSR and RAPD markers for comparative analysis of genetic diversity among different Jatropha curcas genotypes. Afr J Biotechnol 7(23):4230–4343

    CAS  Google Scholar 

  • Hampl V, Pavlícek A, Flegr J (2001) Construction and bootstrap analysis of DNA fingerprinting-based phylogenetic trees with the freeware program Freetree: application to trichomonad parasites. Int J Syst Ecol Mic 51:731–735

    CAS  Google Scholar 

  • Hashizume T, Shimamoto I, Hirai M (2003) Construction of a linkage map and QTL analysis of horticultural traits for watermelon [Citrullus lanatus (THUNB.) MATSUM & NAKAI] using RAPD, RFLP and ISSR markers. Theor Appl Genet 106:779–785

    CAS  PubMed  Google Scholar 

  • Heller J (1996) Physic nut, Jatropha curcas L. Promoting the conservation and use of underutilized and neglected crops. 1. Institute of Plant Genetics and Crop Plant Research, Gartersleben

    Google Scholar 

  • Joshi-Saha A, Gopalakrishna T (2007) Inheritance and tagging of gene regulating flowering time in the green manure crop Sesbania rostrata (Bremek. & Obrem.). Mol Breed 20:389–399

    Article  Google Scholar 

  • Kimura I, Crow J (1964) The number of alleles that can be maintained in a finite population. Genetics 49:725–738

    CAS  PubMed  Google Scholar 

  • King AJ, He W, Cuevas JA, Freudenberger M, Ramiaramanana D, Graham IA (2009) Potential of Jatropha curcas as a source of renewable oil and animal feed. J Exp Bot 13:1–9

    Google Scholar 

  • Lewontin RC (1972) The apportionment of human diversity. Evol Biol 6:381–398

    Google Scholar 

  • Li Z, Nelson RL (2002) RAPD marker diversity among cultivated and wild soybean accessions from four Chinese provinces. Crop Sci 42:1737–1744

    Article  Google Scholar 

  • Makkar HPS, Becker K, Schmook B (1998) Edible provenances of Jatropha curcas from Quintana Roo state of Mexico and effect of roasting on antinutrient and toxic factors in seeds. Plant Foods Hum Nutr 52:31–36

    Article  CAS  PubMed  Google Scholar 

  • Martins-Lopes P, Lima-Brito J, Gomes S, Meirinhos J, Santos L, Guedes-Pinto H (2007) RAPD and ISSR molecular markers in Olea europaea L.: genetic variability and molecular cultivar identification. Genet Resour Crop Evol 54:117–128

    Article  CAS  Google Scholar 

  • McDermott JM, McDonald BA (1993) Gene flow in plant pathosystems. Annu Rev Phytopathol 31:353–373

    Article  Google Scholar 

  • Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70:3321–3323

    Article  CAS  PubMed  Google Scholar 

  • Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590

    CAS  PubMed  Google Scholar 

  • Openshaw K (2000) A review of Jatropha curcas: an oil plant of unfulfilled promise. Biomass Bioenergy 19(1):1–15

    Article  Google Scholar 

  • Parthiban KT, Kumar RS, Thiyagarajan P, Subbulakshmi V, Vennila S, Rao MG (2009) Hybrid progenies in jatropha—a new development. Curr Sci 96(6):815–823

    CAS  Google Scholar 

  • Peakall R, Smouse PE (2006) GenAIEx 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295

    Article  Google Scholar 

  • Prabakaran AJ, Sujatha M (1999) Jatropha tanjorensis Ellis & Saroja, a natural interspecific hybrid occurring in Tamil Nadu, India. Genet Resour Crop Evol 46:213–218

    Article  Google Scholar 

  • Qi X, Pittaway TS, Lindup S, Liu H, Waterman E, Padi FK, Hash CT, Zhu J, Gale MD, Devos KM (2004) An integrated genetic map and a new set of simple sequence repeat markers for pearl millet, Pennisetum glaucum. Theor Appl Genet 109:1485–1493

    Article  CAS  PubMed  Google Scholar 

  • Ranade SA, Srivastava AP, Rana TS, Srivastava J, Tuli R (2008) Easy assessment of diversity in Jatropha curcas L. plants using two single-primer amplification reaction (SPAR) methods. Biomass Bioenergy 32:533–540

    Article  CAS  Google Scholar 

  • Ratha Krishnan P, Paramathma M (2009) Potentials and Jatropha species wealth of India. Curr Sci 97(7):1000–1004

    Google Scholar 

  • Reddy MP, Sarlan N, Siddiq EA (2002) Inter-simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 128:9–17

    Article  Google Scholar 

  • Rohlf FJ (1998) On applications of geometric morphometrics to studies of ontogeny and phylogeny. Syst Biol 47:147–158

    Article  CAS  PubMed  Google Scholar 

  • Rohlf FJ, Sokal RR (1981) Comparing numerical taxonomic studies. Syst Zool 30:459–490

    Article  Google Scholar 

  • Sankar AA, Moore GA (2001) Evaluation of inter-simple sequence repeat analysis for mapping in citrus and extension of the genetic linkage map. Theor Appl Genet 102:206–214

    Article  CAS  Google Scholar 

  • Sehgal D, Rajpal VR, Raina SN, Sasanuma T, Sasakuma T (2009) Assaying polymorphism at DNA sequence level for new and novel genetic diversity diagnostics of the safflower (Carthamus tinctorius L.) world germplasm resources. Genetica 135(3):457–470

    Article  CAS  PubMed  Google Scholar 

  • Semagn K, Bjørnstad Å, Ndjiondjop MN (2006) An overview of molecular marker methods for plants. Afr J Biotechnol 5(25):2540–2568

    CAS  Google Scholar 

  • Senthil Kumar R, Parthiban KT, Govinda Rao M (2009) Molecular characterization of Jatropha genetic resources through inter-simple sequence repeat (ISSR) markers. Mol Biol Rep 36:1951–1956

    Article  CAS  PubMed  Google Scholar 

  • Shannon CE, Weaver W (1949) The mathematical theory of communication. University of Illinois Press, Urbana

    Google Scholar 

  • Sokal RR, Michener CD (1958) Statistical method for evaluating systematic relationships. Univ Kans Sci Bull 38:1409–1438

    Google Scholar 

  • Sokal RR, Sneath PHA (1963) Principle of numerical taxonomy. Freeman, San Francisco

    Google Scholar 

  • Sudheer Pamidimarri DVN, Pandya N, Reddy MP, Radhakrishnan T (2009a) Comparative study of interspecific genetic divergence and phylogenic analysis of genus Jatropha by RAPD and AFLP. Mol Biol Rep 36:901–907

    Article  Google Scholar 

  • Sudheer Pamidimarri DVN, Singh S, Mastan SG, Patel J, Reddy MP (2009b) Molecular characterization and identification of markers for toxic and non-toxic varieties of Jatropha curcas L. using RAPD, AFLP and SSR markers. Mol Biol Rep 36:1357–1364

    Article  CAS  PubMed  Google Scholar 

  • Sujatha M, Prabakaran AJ (2003) New ornamental Jatropha hybrids through interspecific hybridization. Genet Resour Crop Evol 50:75–82

    Article  CAS  Google Scholar 

  • Tatikonda L, Wani SP, Kannan S, Beerelli N, Sreedevi TK, Hoisington DA, Devi P, Varshney RK (2009) AFLP-based molecular characterization of an elite germplasm collection of Jatropha curcas L., a biofuel plant. Plant Sci 176:505–513

    Article  CAS  Google Scholar 

  • Van der Nest MA, Steenkamp ET, Wingfield BD, Wingfield MJ (2000) Development of simple sequence repeat (SSR) markers in Eucalyptus from amplified inter-simple sequence repeats (ISSR). Plant Breed 119:433–436

    Article  Google Scholar 

  • Venkateswarlu M, Raje Urs S, Surendra Nath B, Shashidhar HE, Maheswaran M, Veeraiah TM, Sabitha MG (2006) A first genetic linkage map of mulberry (Morus spp.) using RAPD, ISSR, and SSR markers and pseudotestcross mapping. Tree Genetics & Genomes 3:15–24

    Article  Google Scholar 

  • Vijayan K, Srivatsava PP, Nair CN, Awasthi AK, Tikader A, Sreenivasa B, Urs SR (2006) Molecular characterization and identification of markers associated with yield traits in mulberry using ISSR markers. Plant Breed 125:298–301

    Article  CAS  Google Scholar 

  • Wang A, Yu Z, Ding Y (2009) Genetic diversity analysis of wild close relatives of barley from Tibet and the Middle East by ISSR and SSR markers. C R Biologies 332:393–403

    Article  CAS  PubMed  Google Scholar 

  • Yeh FC, Yang RC, Boyle T (1999) POPGENE Version 1.31, Microsoft Window-based freeware for population genetic analysis. University of Alberta and Centre for International Forestry Research

Download references

Acknowledgment

The authors thank Protector Nutrition Co., Ltd. Thailand and Center for Advanced Studies in Agriculture and Food, Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand for financially supporting this work. We also thank Center for Agricultural Biotechnology, Kasetsart University for lab facility.

Author information

Authors and Affiliations

  1. Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand

    Patcharin Tanya, Puntaree Taeprayoon, Yaowanart Hadkam & Peerasak Srinives

Authors
  1. Patcharin Tanya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Puntaree Taeprayoon
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yaowanart Hadkam
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Peerasak Srinives
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Peerasak Srinives.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tanya, P., Taeprayoon, P., Hadkam, Y. et al. Genetic Diversity Among Jatropha and Jatropha-Related Species Based on ISSR Markers. Plant Mol Biol Rep 29, 252–264 (2011). https://doi.org/10.1007/s11105-010-0220-2

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11105-010-0220-2

Keywords

Search

Navigation