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Characterization of Defense Signaling Pathways of Brassica napus and Brassica carinata in Response to Sclerotinia sclerotiorum Challenge

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Abstract

Canola (Brassica napus L.) is an agriculturally and economically important crop in Canada, and its growth and yield are frequently influenced by fungal pathogens. Sclerotinia sclerotiorum is among those fungal pathogens and causes stem rot disease in B. napus whereas it has been reported that Brassica carinata is moderately tolerant to S. sclerotiorum. Jasmonic acid/ethylene (JA/ET) and salicylic acid (SA) are phytohormones that are known to be involved in plant disease responses. To investigate the defense signaling cascades involved in the interaction of B. napus and B. carinata with S. sclerotiorum, we examined the expression of five orthologs of B. napus genes involved in JA/ET or SA signaling pathways using quantitative RT-PCR. Our results indicated that there are differences in the timing of JA/ET and SA signaling pathways between B. napus and B. carinata. Our results in these two Brassica species also support previous observations that necrotrophic pathogens trigger JA/ET signaling in response to infection. Finally, we observed that transgenic canola expressing 1-aminocyclopropane-1-carboxylate-deaminase producing low levels of ET was relatively more susceptible to S. sclerotiorum than its wild-type counterpart, suggesting that ET inhibits S. sclerotiorum-induced symptom development.

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Acknowledgements

We thank the technical help of Drs. Yuanqing Jiang and Bill Yajima for critical reading of this manuscript. This work was supported by the Alberta Agricultural Research Institute (grant number 2004A001R).

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  1. Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada

    Bo Yang, Muhammad H. Rahman, Yue Liang & Nat N. V. Kav

  2. Alberta Research Council, Bag 4000, Vegreville, AB, T9C 1T4, Canada

    Saleh Shah

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  1. Bo Yang
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  2. Muhammad H. Rahman
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Correspondence to Nat N. V. Kav.

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Both Muhammad Rahman and Yue Liang contributed equally.

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Supplementary Fig. 1

Mutiple alignments of cDNA/amino acid sequence of different alleles of genes AOC3, ERF2, and TGA5 by BOXSHADE3.21 (http://www.ch.embnet.org/software/BOX_form.html). (PDF 35 kb)

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Yang, B., Rahman, M.H., Liang, Y. et al. Characterization of Defense Signaling Pathways of Brassica napus and Brassica carinata in Response to Sclerotinia sclerotiorum Challenge. Plant Mol Biol Rep 28, 253–263 (2010). https://doi.org/10.1007/s11105-009-0149-5

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