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Caterpillar-Specific Gene Expression in the Legume, Medicago truncatula

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Abstract

Plants adjust their defense responses to target caterpillar herbivores. Their ability to do this is, in part, based on the recognition of salivary elicitors which are secreted during insect feeding. We are interested in identifying caterpillar-specific defense responses in the model legume, Medicago truncatula Gaertn. (Fabaceae). To do this, we have taken advantage of the fact that labial salivary secretions of caterpillars of the beet armyworm, Spodoptera exigua Hübner (Lepidoptera: Noctuidae), can be impaired by cauterizing the spinneret. Plant tissues were removed within 1 h after the initiation of herbivory, and differentially expressed transcripts were identified by cDNA-amplified fragment length polymorphism (cDNA-AFLP). Gene expression patterns of five differentially expressed transcripts and one constitutive gene were verified by quantitative real time-polymerase chain reaction (qRT-PCR). Genes encoding ribulose-1,5-bisphosphate carboxylase/oxygenase activase (rubisco activase; MtRCA), strictosidine synthase (MtSTR), an unknown protein (MtUNK), a zinc RING finger protein (MtRFP), and a receptor-like protein kinase (MtRPK) were constitutively expressed. In plants subject to mechanical damage or herbivory by caterpillars, lower expression of MtRCA, MtSTR, Mt UNK, and MtRFP was observed. In comparison, higher levels of MtRPK were expressed in a caterpillar-specific manner.

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Abbreviations

Cat:

caterpillars with impaired or intact salivary secretions

Caut:

caterpillars with impaired salivary secretions

cDNA-AFLP:

complimentary DNA-amplified fragment length polymorphism

Intact:

caterpillars with intact salivary secretions

GOX:

glucose oxidase

PCR:

polymerase chain reaction

MtEF :

gene encoding elongation factor

MtRCA :

gene encoding rubsico activase

MtRCA :

gene encoding rubsico activase

MtRFP :

gene encoding a zinc finger transcription factor

MtRPK :

gene encoding a receptor-like protein kinase

MtSTR :

gene encoding strictosidine synthase

MtUNK :

gene encoding an unknown protein

qRT-PCR:

quantitative real time-PCR

wnd:

wound

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Acknowledgments

We thank Yao Hua Law and Bejay Mills for caterpillar maintenance. We thank Dr. Korban and an anonymous review for insightful comments on an earlier version of this manuscript. This research was supported through grants from the National Sciences and Engineering Research Council, Infrastructure Operating Fund and Fonds Québécois de la Recherche sur la Nature et les Technologies (FQRNT). Instruments used in this research were purchased through grants from the Canadian Foundation for Innovation.

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  1. Department of Plant Science, McGill University, 21,111 Lakeshore, Ste-Anne-de-Bellevue, QC, Canada, H9X 3V9

    Shireef A. Darwish, Li Pan, Christine Ide & Jacqueline C. Bede

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  1. Shireef A. Darwish
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Correspondence to Jacqueline C. Bede.

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Darwish, S.A., Pan, L., Ide, C. et al. Caterpillar-Specific Gene Expression in the Legume, Medicago truncatula . Plant Mol Biol Rep 26, 12–31 (2008). https://doi.org/10.1007/s11105-008-0019-6

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  • DOI: https://doi.org/10.1007/s11105-008-0019-6

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