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A tomato glutaredoxin gene SlGRX1 regulates plant responses to oxidative, drought and salt stresses

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Abstract

Glutaredoxins (Grxs) are ubiquitous small heat-stable disulfide oxidoreductases that play a crucial role in plant development and response to oxidative stress. Here, a novel cDNA fragment (SlGRX1) from tomato encoding a protein containing the consensus Grx family domain with a CGFS active site was isolated and characterized. Southern blot analysis indicated that SlGRX1 gene had a single copy in tomato genome. Quantitative real-time RT-PCR analysis revealed that SlGRX1 was expressed ubiquitously in tomato including leaf, root, stem and flower, and its expression could be induced by oxidative, drought, and salt stresses. Virus-induced gene silencing mediated silencing of SlGRX1 in tomato led to increased sensitivity to oxidative and salt stresses with decreased relative chlorophyll content, and reduced tolerance to drought stress with decreased relative water content. In contrast, over-expression of SlGRX1 in Arabidopsis plants significantly increased resistance of plants to oxidative, drought, and salt stresses. Furthermore, expression levels of oxidative, drought and salt stress related genes Apx2, Apx6, and RD22 were up-regulated in SlGRX1-overexpressed Arabidopsis plants when analyzed by quantitative real-time PCR. Our results suggest that the Grx gene SlGRX1 plays an important role in regulating abiotic tolerance against oxidative, drought, and salt stresses.

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Abbreviations

MS-medium:

Murashige and Skoog medium

NCBI:

National center for biotechnology information

PCR:

Polymerase chain reaction

RT-PCR:

Reverse transcription PCR

VIGS:

Virus-induced gene silencing

TYLCCNV:

Tomato yellow leaf curl China virus

Grx:

Glutaredoxin

TMV:

Tobacco mosaic virus

CaMV:

Cauliflower mosaic virus

qRT-PCR:

Quantitative real-time RT-PCR

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Acknowledgments

This work was financially supported by the Important National Science & Technology Specific Projects of China (2009ZX08009-026B), the National Key Basic Research and Development Program of China (2006CB101903) and the National High Technology Research and Development program of China (863 Program) (2007AA10Z413).

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  1. State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, 310029, Zhejiang, China

    Yushuang Guo, Changjun Huang, Yan Xie, Fengming Song & Xueping Zhou

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  1. Yushuang Guo
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  3. Yan Xie
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  5. Xueping Zhou
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Correspondence to Xueping Zhou.

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Guo, Y., Huang, C., Xie, Y. et al. A tomato glutaredoxin gene SlGRX1 regulates plant responses to oxidative, drought and salt stresses. Planta 232, 1499–1509 (2010). https://doi.org/10.1007/s00425-010-1271-1

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