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Response of mycorrhizal grapevine to Armillaria mellea inoculation: disease development and polyamines

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Abstract

A study was conducted with the vine rootstock Richter 110 (Vitis berlandieri Planch. x Vitis rupestris L.) in order to assess whether the colonisation by the arbuscular mycorrhizal fungus (AMF) Glomus intraradices (BEG 72) can delay the disease development in plants inoculated with the root-rot fungus Armillaria mellea (Vahl:Fr) Kummer, and to elucidate if the levels of polyamines (PAs) are modified in response to G. intraradices, A. mellea or by the dual infection. Four treatments were considered: control and G. intraradices-inoculated plants infected or not with A. mellea. Plant growth, mycorrhizal colonisation and disease development were monitored throughout the experiment. High performance liquid chromatography (HPLC) in combination with fluorescence spectrophotometry was used to separate and quantify free root and leaf polyamines. The slower development of pathogenic symptoms and the higher plant biomass of mycorrhizal plants inoculated with A. mellea indicate an increase of tolerance due to the AMF inoculation. The variations in free PA levels detected at the beginning of the pathogenic infection suggest that PAs may have a potential role in the signalling mechanisms of the tolerance of mycorrhizal plants against A. mellea.

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Acknowledgements

Financial support for this research was provided by the Instituto de Investigación y Tecnología Agraria y Alimentaria (INIA), project RTA-04-027-C2-1, by the “Departament d’Universitats, Recerca i Societat de la Informació de la Generalitat de Catalunya” and by the European Regional Development Fund.

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Authors and Affiliations

  1. Institut de Recerca I Tecnologia Agroalimentàries (IRTA), Ctra. de Cabrils km 2, 08348, Cabrils, Barcelona, Spain

    Amaia Nogales, Amèlia Camprubí & Cinta Calvet

  2. Departamento Biología Vegetal, Facultades de Ciencias y Farmacia, Universidad de Navarra, c/Irunlarrea n° 1, 31008, Pamplona, Navarra, Spain

    Jone Aguirreolea & Eva Santa María

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  1. Amaia Nogales
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  2. Jone Aguirreolea
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  3. Eva Santa María
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Correspondence to Amaia Nogales.

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Responsible Editor: Harsh P. Bais.

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Nogales, A., Aguirreolea, J., Santa María, E. et al. Response of mycorrhizal grapevine to Armillaria mellea inoculation: disease development and polyamines. Plant Soil 317, 177–187 (2009). https://doi.org/10.1007/s11104-008-9799-6

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