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Decreasing prevalence of rhizosphere IAA producing and seedling root growth promoting bacteria with barley development irrespective of protozoan grazing regime

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Abstract

Barley was grown in soil with either bacteria and a mixed protozoan community (Mixed protozoa) or bacteria and a single vahlkampfiid amoebal species (Single amoeba). We assessed the influence of plant age (day 29, 43 and 57 after sowing) on two aspects of rhizosphere bacterial functioning: (1) the proportion of indole-3-acetic acid (IAA) producing bacteria and (2) the effect of mixed rhizosphere bacterial assemblages on barley seedling root growth in an agar based assay. The proportion of IAA producers was significantly lower at day 57 than at day 29 and 43, and mixed bacterial assemblages extracted from rhizospheres of 29 days old plants were significantly less harmful to seedling growth than bacterial assemblages from older plants. Hence both assays indicated that bacterial communities from rhizospheres of older plants were less beneficial for root growth than bacterial communities from younger plants. Genetic fingerprinting of rhizosphere bacterial communities was compared by use of length heterogeneity polymerase chain reaction (LH-PCR). This analysis showed a clear succession from the inoculum bacterial community with a rather low diversity to a community with much higher diversity at day 29. However, diversity did not change after day 29, and no relationship between protozoan treatment nor plant age and genetic fingerprinting was found.

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References

  • Ashgar HN, Zahir ZA, Arshad M, Khaliq A (2002) Relationship between in vitro production of auxins by rhizobacteria and their growth-promoting activities in Brassica juncea L. Biol Fertil Soils 35:231–237

    Article  Google Scholar 

  • Åström B, Gustafsson A, Gerhardson B (1993) Characteristics of a plant deleterious rhizosphere pseudomonad and its inhibitory metabolite(s). J Appl Bacteriol 74:20–28

    Google Scholar 

  • Barbieri P, Galli E (1993) Effect of wheat root development of inoculation with an Azospirillum brasiliense mutant with altered indole-3- acetic acid production. Res Microbiol 144:69–75

    Article  PubMed  Google Scholar 

  • Bjørnlund L, Mørk S, Vestergård M, Rønn R (2006) Trophic interactions between rhizosphere bacteria and bacterial feeders influenced by phosphate and aphids in barley. Biol Fertil Soils 43:1–11

    Article  Google Scholar 

  • Bonkowski M, Brandt F (2002) Do soil protozoa enhance plant growth by hormonal effects? Soil Biol Biochem 34:1709–1715

    Article  Google Scholar 

  • Bric JM, Bostock RM, Silverstone SE (1991) Rapid in situ assay for indoleacetic acid production by bacteria immobilized on a nitrocellulose membrane. Appl Environ Microbiol 57:535–538

    PubMed  Google Scholar 

  • Bååth E, Olsson S, Tunlid A (1988) Growth of bacteria in rhizoplane and rhizosphere of rape seedlings. FEMS Microbiol Ecol 53:355–360

    Article  Google Scholar 

  • Campbell R, Greaves MP (1990) Anatomy and community structure of the rhizosphere. In: Lynch JM (ed) The Rhizosphere. John Wiley & Sons, Chichester, pp 11–34

    Google Scholar 

  • Chakrabarti T, Doy CH, Subrahmanyam NC (1978) The analysis of barley genomes. I: The problem that the DNA of bacteria may contribute to the DNA in extracts of barley tissues derived from germinated seeds. Barley Genetics Newsletter 8:25–28

    Google Scholar 

  • Christensen S, Bjørnlund L, Vestergård M (2007) Decomposer biomass in the rhizosphere to assess rhizodeposition. Oikos 116:65–74

    Article  Google Scholar 

  • Clarholm M (1985) Interactions of bacteria, protozoa and plants leading to mineralization of soil nitrogen. Soil Biol Biochem 17:181–187

    Article  Google Scholar 

  • Curl EA, Truelove B (1986) The rhizosphere. Springer-Verlag, Berlin

    Google Scholar 

  • Darbyshire JF, Wheatley RE, Greaves MP, Inkson RHE (1974) A rapid micromethod for estimating bacterial and protozoan populations in soil. Rev Écol Biol Sol 11:465–475

    Google Scholar 

  • Dobbelaere S, Croonenborghs A, Thys A, Vande Broek A, Vanderleyden J (1999) Phytostimulatory effect of Azospirillum brasiliense wild type and mutant strains altered in IAA production on wheat. Plant Soil 212:155–164

    Article  Google Scholar 

  • Frankenberger WT, Arshad M (1995) Phytohormones in soils. Microbial production and function. Marcel Dekker, Inc., New York

    Google Scholar 

  • Frederickson JK, Elliott LF (1985) Effects on winter wheat seedling growth by toxin-producing rhizobacteria. Plant Soil 83:399–409

    Article  Google Scholar 

  • Fægri A, Torsvik VL, Goksöyr J (1977) Bacterial and fungal activities in soil: Separation of bacteria and fungi by a rapid fractionated centrifugation technique. Soil Biol Biochem 9:105–112

    Article  Google Scholar 

  • Glick BR, Changping L, Ghosh S, Dumbroff EB (1997) Early development of Canola seedlings in the presence of the plant growth-promoting rhizobacterium Pseudomonas putida GR12-2. Soil Biol Biochem 29:1233–1239

    Article  Google Scholar 

  • Glick BR, Penrose DM, Li J (1998) A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria. J Theor Biol 190:63–68

    Article  Google Scholar 

  • Gregory PJ, Atwell BJ (1991) The fate of carbon in pulse-labelled crops of barley and wheat. Plant Soil 136:205–213

    Article  Google Scholar 

  • Griffiths BS (1990) A comparison of microbial-feeding nematodes and protozoa in the rhizosphere of different plants. Biol Fertil Soils 9:83–88

    Article  Google Scholar 

  • Griffiths BS, Bonkowski M, Dobson G, Caul S (1999) Changes in soil microbial community structure in the presence of microbial-feeding nematodes and protozoa. Pedobiologia 43:297–304

    Google Scholar 

  • Hankes LV, Riesen WH, Henderson LM, Elvehjem CA (1948) Liberation of amino acids from raw and heated casein by acid and enzyme hydrolysis. J Biol Chem 176:467–476

    Google Scholar 

  • Henry F, Nguyen C, Paterson E, Sim A, Robin C (2005) How does nitrogen availability alter rhizodeposition in Lolium multiflorum Lam. during vegetative growth? Plant Soil 269:181–191

    Article  Google Scholar 

  • Jensen B (1993) Rhizodeposition by 14CO2-pulse-labelled spring barley grown in small field plots on sandy loam. Soil Biol Biochem 25:1553–1559

    Article  Google Scholar 

  • Kandeler E, Marschner P, Tscherko D, Gahoonia TS, Nielsen NE (2002) Microbial community composition and functional diversity in the rhizosphere of maize. Plant Soil 238:301–312

    Article  Google Scholar 

  • Kowalchuk GA, Buma DS, de Boer W, Klinkhamer PGL, van Veen JA (2002) Effects of above-ground plant species composition and diversity on the diversity of soil-borne microorganisms. Antonie van Leeuwenhoek 81:509–520

    Article  PubMed  Google Scholar 

  • Krasnoff SB, Lobkovsky EB, Wach MJ, Loria R, Gibson DM (2005) Chemistry and phytotoxicity of thaxtomin A alkyl esters. J Agric Food Chem 53:9446–9451

    Article  PubMed  Google Scholar 

  • Liu WT, Cheng H, Forney LJ (1997) Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA. Appl Environ Microbiol 63:4516–4522

    PubMed  Google Scholar 

  • Lottmann J, Heuer H, Smalla K, Berg G (1999) Influence of transgenic T4-lysozyme-producing potato plants on potentially beneficial plant-associated bacteria. FEMS Microbiol Ecol 29:365–377

    Article  Google Scholar 

  • Magurran AE (2003) Measuring biological diversity. Blackwell Science Ltd, Malden

    Google Scholar 

  • Marschner P, Yang C-H, Lieberei R, Crowley DE (2001) Soil and plant specific effects on bacterial community composition in the rhizosphere. Soil Biol Biochem 33:1437–1445

    Article  Google Scholar 

  • Marschner P, Crowley D, Yang CH (2004) Development of specific rhizosphere bacterial communities in relation to plant species, nutrition and soil type. Plant Soil 261:199–208

    Article  Google Scholar 

  • Mougel C, Offre P, Ranjard L, Corberand T, Gamalero E, Robin C, Lemanceau P (2006) Dynamic of the genetic structure of bacterial and fungal communities at different developmental stages of Medicago truncatula Gaertn. Cv. Jemalong line J5. New Phytol 170:165–175

    Article  PubMed  Google Scholar 

  • Murase J, Noll M, Frenzel P (2006) Impact of protists on the activity and structure of the bacterial community in a rice field soil. Appl Environ Microbiol 72:5436–5444

    Article  PubMed  Google Scholar 

  • Olsen RA, Bakken LR (1987) Viability of soil bacteria: optimization of the plate-counting technique. Microb Ecol 13:59–74

    Article  Google Scholar 

  • Page FC (1988) A new key to freshwater and soil gymnamoebae. Freshwater Biological Association, Ambleside

    Google Scholar 

  • Patten CL, Glick BR (2002) Role of Pseudomonas putida indoleacetic acid in development of the host plant root system. Appl Environl Microbiol 68:3795–3801

    Article  Google Scholar 

  • Picard C, Bosco M (2003) Soil antimony pollution and plant growth stage affect the biodiversity of auxin-producing bacterial isolated from the rhizosphere of Achillea agaretum L. FEMS Microbiol Ecol 46:73–80

    Article  Google Scholar 

  • Priemé A, Sitaula JIB, Klemedtsson ÅK, Bakken LR (1996) Extraction of methane-oxidizing bacteria from soil particles. FEMS Microbiol Ecol 21:59–68

    Article  Google Scholar 

  • de Ridder-Duine AS, Kowalchuk GA, Gunnewiek PJAK, Smant W, van Veen JA, de Boer W (2005) Rhizosphere bacterial community composition in natural stands of Carex arenaria (sand sedge) is determined by bulk soil community composition. Soil Biol& Biochem 37:349–357

    Article  Google Scholar 

  • Ritchie NJ, Schutter ME, Dick RP, Myrold DD (2000) Use of Length Heterogeneity PCR and Fatty Acid Methyl Ester profiles to characterize microbial communities in soil. Appl Environ Microbiol 66:1668–1675

    Article  PubMed  Google Scholar 

  • Rovira AD, Newman EI, Bowen HJ, Campbell R (1974) Quantitative assessment of the rhizoplane microflora by direct microscopy. Soil Biol Biochem 6:211–216

    Article  Google Scholar 

  • Rønn R, Ekelund F, Christensen S (1995) Optimizing soil extract and broth media for MPN-enumeration of naked amobae and heterotrophic flagellates in soil. Pedobiologia 39:10–19

    Google Scholar 

  • Rønn R, McCaig AE, Griffiths BS, Prosser JI (2002) Impact of protozoan grazing on bacterial community structure in soil microcosms. Appl Environl Microbiol 68:6094–6105

    Article  Google Scholar 

  • de Salamone IEG, Hynes RK, Nelson LM (2001) Cytokinin production by plant growth promoting rhizobacteria and selected mutants. Can J Microbiol 47:404–411

    Article  Google Scholar 

  • Schippers B, Bakker AW, Bakker PAHM, van Peer R (1990) Beneficial and deleterious effects of HCN-producing pseudomonads on rhizosphere interactions. Plant Soil 129:75–83

    Article  Google Scholar 

  • Swinnen J (1994) Rhizodeposition and turnover of root–derived organic material in barley and wheat under conventional and integrated management. Agric Ecosyst Environ 51:115–128

    Article  Google Scholar 

  • Swinnen J, van Veen JA, Merckx R (1995) Carbon fluxes in the rhizosphere of winter wheat and spring barley with conventional vs integrated farming. Soil Biol Biochem 27:811–820

    Article  Google Scholar 

  • Techau MEC, Bjørnlund L, Christensen S (2004) Simulated herbivory effects on rhizosphere organisms in pea (Pisum sativum) depended on phosphate. Plant Soil 264:185–194

    Article  Google Scholar 

  • Timmusk S, Nicander B, Granhall U, Tillberg E (1999) Cytokinin production by Paenibacillus polymyxa. Soil Biol Biochem 31:1847–1852

    Article  Google Scholar 

  • Vestergård M, Bjørnlund L, Christensen S (2004) Aphid effects on rhizosphere microorganisms and microfauna depend more on barley growth phase than on soil fertilization. Oecologia 141:84–93

    Article  PubMed  Google Scholar 

  • Weisskopf L, Fromin N, Tomasi N, Aragno M, Martinoia E (2005) Secretion activity of white lupin’s cluster roots influences bacterial abundance, function and community structure. Plant Soil 268:181–194

    Article  Google Scholar 

  • Wilmotte A, Vanderauwera G, Dewachter R (1993) Structure of the 16S ribosomal-RNA of the thermophilic cyanobacterium Chlorogloeopsis HTF (‘Mastigocladus laminosus HTF’) strain PCC7518, and phylogenetic analysis. FEBS Letter 317:96–100

    Article  Google Scholar 

  • Wolsing M, Priemé A (2004) Observation of high seasonal variation in community structure of denitrifying bacteria in arable soil receiving artificial fertilizer and cattle manure by determining T-RFLP of nir gene fragments. FEMS Microbiol Ecol 48:261–271

    Article  Google Scholar 

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Acknowledgment

We thank Flemming Ekelund for assistance on protozoan identification and Anders Priemé and Karin Vestberg (Section of Microbiology, University of Copenhagen) for running the LH-PCR samples.

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Authors and Affiliations

  1. Section of Terrestrial Ecology, Department of Biology, University of Copenhagen, Øster Farimagsgade 2D, 1353, Copenhagen K, Denmark

    Mette Vestergård, Frédéric Henry & Regin Rønn

  2. Section of Genetics and Microbiology, Department of Ecology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark

    Lisa Bjørnlund

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  1. Mette Vestergård
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  2. Lisa Bjørnlund
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Correspondence to Mette Vestergård.

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Responsible Editor: Petra Marschner

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Vestergård, M., Bjørnlund, L., Henry, F. et al. Decreasing prevalence of rhizosphere IAA producing and seedling root growth promoting bacteria with barley development irrespective of protozoan grazing regime. Plant Soil 295, 115–125 (2007). https://doi.org/10.1007/s11104-007-9267-8

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