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Transcriptome portrait of cellulose-enriched flax fibres at advanced stage of specialization

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Abstract

Functional specialization of cells is among the most fundamental processes of higher organism ontogenesis. The major obstacle to studying this phenomenon in plants is the difficulty of isolating certain types of cells at defined stages of in planta development for in-depth analysis. A rare opportunity is given by the developed model system of flax (Linum usitatissimum L.) phloem fibres that can be purified from the surrounding tissues at the stage of the tertiary cell wall deposition. The performed comparison of the whole transcriptome profile in isolated fibres and other portions of the flax stem, together with fibre metabolism characterization, helped to elucidate the general picture of the advanced stage of plant cell specialization and to reveal novel participants potentially involved in fibre metabolism regulation and cell wall formation. Down-regulation of all genes encoding proteins involved in xylan and lignin synthesis and up-regulation of genes for the specific set of transcription factors transcribed during tertiary cell wall formation were revealed. The increased abundance of transcripts for several glycosyltransferases indicated the enzymes that may be involved in synthesis of fibre-specific version of rhamnogalacturonan I.

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Acknowledgements

This work was partially supported by grants of the Russian Foundation for Basic Research (#14-04-01778; RNA-Seq of isolated fibres, qRT-PCR experiments), the Russian Science Foundation (#16-14-10256; RNA-Seq of top and middle parts of flax stem, 14C-distribution experiments, bioinformatics).

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  1. Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Centre, Russian Academy of Science, Lobachevsky str., 2/31, Kazan, 420111, Russia

    Oleg Gorshkov, Natalia Mokshina, Vladimir Gorshkov, Svetlana Chemikosova, Yuri Gogolev & Tatyana Gorshkova

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  1. Oleg Gorshkov
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Correspondence to Tatyana Gorshkova.

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Gorshkov, O., Mokshina, N., Gorshkov, V. et al. Transcriptome portrait of cellulose-enriched flax fibres at advanced stage of specialization. Plant Mol Biol 93, 431–449 (2017). https://doi.org/10.1007/s11103-016-0571-7

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