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Differences in gene expression amplitude overlie a conserved transcriptomic program occurring between the rapid and potent localized resistant reaction at the syncytium of the Glycine max genotype Peking (PI 548402) as compared to the prolonged and potent resistant reaction of PI 88788

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Abstract

Glycine max L. Merr. (soybean) resistance to Heterodera glycines Ichinohe occurs at the site of infection, a nurse cell known as the syncytium. Resistance is classified into two cytologically-defined responses, the G. max [Peking]- and G. max [PI 88788]-types. Each type represents a cohort of G. max genotypes. Resistance in G. max [Peking] occurs by a potent and rapid localized response, affecting parasitic second stage juveniles (p-J2). In contrast, resistance occurs by a potent but more prolonged reaction in the genotype G. max [PI 88788] that affects nematode development at the J3 and J4 stages. Microarray analyses comparing these cytologically and developmentally distinct resistant reactions reveal differences in gene expression in pericycle and surrounding cells even before infection. The differences include higher relative levels of the differentially expressed in response to arachidonic acid 1 gene (DEA1 [Gm-DEA1]) (+224.19-fold) and a protease inhibitor (+68.28-fold) in G. max [Peking/PI 548402] as compared to G. max [PI 88788]. Gene pathway analyses compare the two genotypes (1) before, (2) at various times during, (3) constitutively throughout the resistant reaction and (4) at all time points prior to and during the resistant reaction. The amplified levels of transcriptional activity of defense genes may explain the rapid and potent reaction in G. max [Peking/PI 548402] as compared to G. max [PI 88788]. In contrast, the shared differential expression levels of genes in G. max [Peking/PI 548402] and G. max [PI 88788] may indicate a conserved genomic program underlying the G. max resistance on which the genotype-specific gene expression programs are built off.

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Abbreviations

EST:

Expressed sequence tag

hpi:

Hours post inoculation

dpi:

Days post inoculation

SCN:

Soybean cyst nematode

J2:

Second stage juvenile

FS:

Farmer’s solution

LCM:

Laser capture microdissection

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Acknowledgments

VPK is a recipient of the Research Initiation Program Grant at Mississippi State University and thankfully acknowledges support provided by the Mississippi Soybean Promotion Board. The authors thank Dr. David Munroe, Nina Bubunenko and Nicole Lum at the Laboratory of Molecular Technology, SAIC-Frederick, National Cancer Institute at Frederick, Frederick, Maryland 21701, USA for the Affymetrix® soybean GeneChip® array hybridizations and data acquisition. Dr. Gary Lawrence Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University provided helpful insight into the analysis of the resistance responses of G. max [Peking/PI 548402] and G. max [PI 88788].

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Authors and Affiliations

  1. Department of Biological Sciences, Harned Hall, Mississippi State University, Mississippi State, MS, 39762, USA

    Vincent P. Klink & Prachi D. Matsye

  2. United States Department of Agriculture, Soybean Genomics and Improvement Laboratory, Bldg. 006, Beltsville, MD, 20705, USA

    Parsa Hosseini & Benjamin F. Matthews

  3. Jess and Mildred Fisher College of Science and Mathematics, Department of Computer and Information Sciences, Towson University, 7800 York Road, Towson, MD, 21252, USA

    Parsa Hosseini & Nadim W. Alkharouf

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  1. Vincent P. Klink
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Klink, V.P., Hosseini, P., Matsye, P.D. et al. Differences in gene expression amplitude overlie a conserved transcriptomic program occurring between the rapid and potent localized resistant reaction at the syncytium of the Glycine max genotype Peking (PI 548402) as compared to the prolonged and potent resistant reaction of PI 88788. Plant Mol Biol 75, 141–165 (2011). https://doi.org/10.1007/s11103-010-9715-3

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  • DOI: https://doi.org/10.1007/s11103-010-9715-3

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