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Identification of genes expressed during the self-incompatibility response in perennial ryegrass (Lolium perenne L.)

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Abstract

Self-incompatibility (SI) in Lolium perenne is controlled gametophytically by the S–Z two-locus system. S and Z loci mapped to L. perenne linkage groups 1 and 2, respectively, with their corresponding putative-syntenic regions on rice chromosome 5 (R5) and R4. None of the gene products of S and Z have yet been identified. SI cDNA libraries were developed to enrich for SI expressed genes in L. perenne. Transcripts were identified from the SI libraries that were orthologous to sequences on rice R4 and R5. These represent potential SI candidate genes. Altogether ten expressed SI candidate genes were identified. A rapid increase in gene expression within two minutes after pollen-stigma contact was revealed, reaching a maximum between 2 and 10 min. The potential involvement of these genes in the SI reactions is discussed.

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Abbreviations

CBL:

Calcineurin B-like

GO:

Gene ontology

GSI:

Gametophytic self-incompatibility

ILGI:

International lolium genome initiative

LG:

Linkage group

MAPK:

Mitogen-activated protein kinase

PCD:

Programmed cell death

PR:

Pathogenesis-related

SCR:

S-Locus cysteine-rich protein

SCP:

Sperm-coating glycoprotein

SI:

Self-incompatibility

SLF:

S-Locus F-box protein

SRK:

S-Locus receptor protein kinase

SSH:

Suppression subtractive hybridization

SSI:

Sporophytic self-incompatibility

STS:

Sequence tagged site

TDF:

Transcribed derived fragment

UBP:

Ubiquitin-specific protease

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Acknowledgments

BY was funded by a Teagasc Walsh Fellowship. We acknowledge core funding contributions of Teagasc and financial contributions of the National Development Plan.

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Authors and Affiliations

  1. Teagasc Crops Research Centre, Oak Park, Carlow, Ireland

    Bicheng Yang & Susanne Barth

  2. Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Plas Gogerddan, Aberystwyth, Ceredigion, SY23 3EB, UK

    Bicheng Yang, Daniel Thorogood & Ian P. Armstead

  3. School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK

    Bicheng Yang & F. C. H. Franklin

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  1. Bicheng Yang
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  2. Daniel Thorogood
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Correspondence to Susanne Barth.

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Accession Number Sequence data from this article can be found in the EMBL/GenBank data libraries under accession numbers AM991118, AM991119, AM991120, AM991121, AM991122, AM991123, AM991124, AM991125, AM991126 and AM991127.

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Yang, B., Thorogood, D., Armstead, I.P. et al. Identification of genes expressed during the self-incompatibility response in perennial ryegrass (Lolium perenne L.). Plant Mol Biol 70, 709–723 (2009). https://doi.org/10.1007/s11103-009-9501-2

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