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OsDMC1 is required for homologous pairing in Oryza sativa

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Abstract

OsDMC1 is the rice homologue of the yeast DMC1 gene. Here, we analyzed the function of OsDMC1 in meiosis using an RNA interference approach. The OsDMC1–RNAi lines grew normally during their vegetative phase but showed spikelet and pollen sterility. The sterility phenotypes were associated with down-regulated OsDMC1 transcript and protein levels mediated by RNAi. Further cytological observations of male meiocytes revealed that knock-down of OsDMC1 led to defects in bivalent formation and subsequent unequal chromosome segregation and irregular spore generation, and induced changes in male meiotic progression. Fluorescent in situ hybridization experiments revealed that the OsDMC1–RNAi lines were defective in homologous pairing. These data indicate that OsDMC1 is essential for rice meiosis and plays an important role in homologous pairing.

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Acknowledgements

We thank Zhukuan Cheng at the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, for assistance with FISH analysis and Cheng Yun at the Institute of Botany, Chinese Academy of Sciences, for assistance with microscope observation. The research was supported by grants from the National Science Foundation of China (Nos. 30270144 and 30570147).

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  1. Research Center of Molecular & Developmental Biology, Key Laboratory of Photosynthesis & Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, Haidianqu, Beijing, 100093, China

    Zhu-Yun Deng & Tai Wang

  2. Graduate School of Chinese Academy of Sciences, Beijing, 100049, China

    Zhu-Yun Deng

  3. National Centre for Plant Gene Research, Beijing, 100093, China

    Tai Wang

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  1. Zhu-Yun Deng
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  2. Tai Wang
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Correspondence to Tai Wang.

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Deng, ZY., Wang, T. OsDMC1 is required for homologous pairing in Oryza sativa. Plant Mol Biol 65, 31–42 (2007). https://doi.org/10.1007/s11103-007-9195-2

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  • DOI: https://doi.org/10.1007/s11103-007-9195-2

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