Skip to main content
SpringerLink
Log in

Monitoring the expression profiles of genes induced by hyperosmotic, high salinity, and oxidative stress and abscisic acid treatment in Arabidopsis cell culture using a full-length cDNA microarray

  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

Transcriptional regulation in response to hyperosmotic, high-salinity and oxidative stress, and abscisic acid (ABA) treatment in Arabidopsis suspension-cultured cell line T87 was investigated with a cDNA microarray containing 7000 independent full-length Arabidopsis cDNAs. The transcripts of 102, 11, 84 and 73 genes were increased more than 5-fold within 5h after treatment with 0.5M mannitol, 0.1M NaCl, 50μM ABA and 10mM H2O2, respectively. On the other hand, the transcripts of 44, 57, 25 and 34 genes were down-regulated to less than one-third within 5h after treatment with 0.5M mannitol, 0.1M NaCl, 50μM ABA and 10mM H2O2, respectively. Venn diagram analysis revealed 11 genes were induced significantly by mannitol, NaCl, and ABA, indicating crosstalk among these signaling pathways. Comparison of the genes induced by each stress revealed that 32%, 17% and 33% of mannitol-, NaCl- and ABA-inducible genes were also induced by H2O2, indicating the crosstalk between the signaling pathways for osmotic stress and oxidative stress. Although the expression profiles revealed that the T87 cells had most of the regulatory systems seen in Arabidopsis seedlings, the T87 cells did not have one of ABA-dependent signaling pathways.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abe, H., Yamaguchi-Shinozaki, K., Urao, T., Iwasaki, T., Hosokawa, D. and Shinozaki, K. 1997. Role of Arabidopsis MYC and MYB homologs in drought-and abscisic acidregulated gene expression. Plant Cell 9: 1859–1868.

    Article  PubMed  CAS  Google Scholar 

  • Abe, H., Urao, T., Seki, M., Shinozaki, K. and Yamaguchi-Shinozaki, K. 2003. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcription activators in abscisic acid signaling. Plant Cell 15: 63–78.

    Article  PubMed  CAS  Google Scholar 

  • Aharoni, A. and Vorst, O. 2002. DNA microarrays for functional plant genomics. Plant Mol. Biol. 48: 99–118.

    Article  PubMed  CAS  Google Scholar 

  • Albrecht, V., Ritz, O., Linder, S., Harter, K. and Kudla, J. 2001. The NAF domain defines a novel protein–protein interaction module conserved in Ca2+-regulated kinases. EMBO J. 20: 1051–1063.

    Article  PubMed  CAS  Google Scholar 

  • Axelos, M., Curie, C., Mazzolini, L., Bardet, C. and Lescure, B. 1992. A protocol for transient gene expression in Arabidopsis thaliana protoplasts isolated from cell suspension cultures. Plant Physiol. Biochem. 30: 123–128.

    CAS  Google Scholar 

  • Banzet, N., Richaud, C., Deveaux, Y., Kazmaier, M., Gagnon, J. and Triantaphylides, C. 1998. Accumulation of small heat shock proteins, including mitochondrial HSP22, induced by oxidative stress and adaptive response in tomato cells. Plant J. 13: 519–527.

    Article  PubMed  CAS  Google Scholar 

  • Bohnert, H. J., Nelson, D. E. and Jensen, R. G. 1995. Adaptations to environmental stresses. Plant Cell 7: 1099–1111.

    Article  PubMed  CAS  Google Scholar 

  • Bonetta, D. and McCourt, P. 1998. Genetic analysis of ABA signal transduction pathways. Trends Plant Sci. 3: 231–235.

    Article  Google Scholar 

  • Bray, E. A. 1997. Plant responses to water deficit. Trends Plant Sci. 2: 48–54.

    Article  Google Scholar 

  • Chen, W., Chao, G. and Singh, K. B. 1996. The promoter of a H2O2-inducible, Arabidopsis glutathione S-transferase gene contains closely linked OBF-and OBP1-binding sites. Plant J. 10: 955–966.

    Article  PubMed  CAS  Google Scholar 

  • Choi, H., Hong, J., Ha, J., Kang, J. and Kim, S. Y. 2000. ABFs, a family of ABA-responsive element binding factors J. Biol. Chem. 275: 1723–1730.

    CAS  Google Scholar 

  • Cutler, A. J. and Krochko, J. E. 1999. Formation and breakdown of ABA. Trends Plant Sci. 4: 472–478.

    Article  PubMed  Google Scholar 

  • Desikan, R., A-H-Mackerness, S., Hancock, J. T. and Neill, S. J. 2001. Regulation of the Arabidopsis transcriptome by oxidative stress. Plant Physiol. 127: 159–172.

    Article  PubMed  CAS  Google Scholar 

  • Eulgem, T., Rushton, P. J., Robatzek, S. and Somssich, I. E. 2000. The WRKY superfamily of plant transcription factors. Trends Plant Sci. 5: 199–206.

    Article  PubMed  CAS  Google Scholar 

  • Eulgem, T., Rushton, P. J., Schmelzer, E., Hahlbrock, K. and Somssich, I. E. 1999. Early nuclear events in plant defence signaling: rapid gene activation by WRKY transcription factors. EMBO J. 18: 4689–4699.

    Article  PubMed  CAS  Google Scholar 

  • Finkelstei, D., Ewing, R., Gollub, J., Sterky, F., Cherry, J. M. and Somerville, S. 2002. Microarray data quality analysis: lessons from the AFGC project. Arabidopsis Functional Genomics Consortium. Plant Mol. Biol. 48: 119–131.

    Article  Google Scholar 

  • Fowler, S. and Thomashow, M. F. 2002. Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. Plant Cell 14: 1675–1690.

    Article  PubMed  CAS  Google Scholar 

  • Grill, E. and Himmelbach, A. 1998. ABA signal transduction. Curr. Opin. Plant Biol. 1: 412–418.

    Article  PubMed  CAS  Google Scholar 

  • Guan, L. and Scandalios, J. G. 1993. Characterization of the catalase antioxidant defense gene Cat1 of maize, and its developmentally regulated expression in transgenic tobacco. Plant J. 3: 527–536.

    Article  PubMed  CAS  Google Scholar 

  • Guan, L. M., Zhao, J. and Scandalios, J. G. 2000. Cis-elements and trans-factors that regulate expression of the maize Cat1 antioxidant gene in response to ABA and osmotic stress: 53 H2O2 is the likely intermediary signaling molecule for the response. Plant J. 22: 87–95.

    Article  PubMed  CAS  Google Scholar 

  • Harper, J. F., Manney, L., DeWitt, N. D., Yoo, M. H. and Sussman, M. R. 1990. The Arabidopsis thaliana plasma membrane H(+)-ATPase multigene family. Genomic sequence and expression of a third isoform. J. Biol. Chem. 265: 13601–13608.

    PubMed  CAS  Google Scholar 

  • Hasegawa, P. M., Bressan, R. A., Zhu, J. K. and Bohnert, H. J. 2000. Plant cellular and molecular responses to high salinity. Annu. Rev. Plant Physiol. Plant Mol. Biol. 51: 463–499.

    Article  CAS  Google Scholar 

  • Hazen, S. P., Wu, Y. and Kreps, J. A. 2003. Gene expression profiling of plant responses to abiotic stress. Funct. Integr. Genomics 3: 105–111.

    Article  PubMed  CAS  Google Scholar 

  • Higo, K., Ugawa, Y., Iwamoto, M. and Korenaga, T. 1999. Plant cis-acting regulatory DNA elements (PLACE) database: 1999. Nucleic Acids Res. 27: 297–300.

    Article  PubMed  CAS  Google Scholar 

  • Himmelbach, A., Item, M. and Grill, E. 1998. Signaling of abscisic acid to regulate plant growth. Philos. Trans. R. Soc. Lond. R. Biol. Sci. 353: 1439–1444.

    Article  CAS  Google Scholar 

  • Ingram, J. and Bartels, D. 1996. The molecular basis of dehydration tolerance in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47: 377–403.

    Article  PubMed  CAS  Google Scholar 

  • Jouanneau, J.-P. and Pe´ aud-Lenoe¨ l, C. 1967. Croissance et synthese des prote´ ines de suspensions cellulaires de tabac sensibles ala kine´ tine. Physiol. Plant. 20: 834–850.

    Article  CAS  Google Scholar 

  • Kawasaki, S., Borchert, C., Deyholos, M., Wang, H., Brazille, S., Kawai, K., Galbraith, D. and Bohnert, H. J. 2001. Gene expression profiles during the initial phase of salt stress in rice. Plant Cell 13: 889–905.

    Article  PubMed  CAS  Google Scholar 

  • Kiyosue, T., Yamaguchi-Shinozaki, K. and Shinozaki, K. 1994. Cloning of cDNAs for genes that are early-responsive to dehydration stress (ERDs) in Arabidopsis thaliana L.: identification of three ERDs as HSP cognate genes. Plant Mol. Biol. 25: 791–798.

    Article  PubMed  CAS  Google Scholar 

  • Kreps, J. A., Wu, Y., Chang, H. S., Zhu, T., Wang, X. and Harper, J. F. 2002. Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiol. 130: 2129–2141.

    Article  PubMed  CAS  Google Scholar 

  • Kurkela, S. and Borg-Franck, M. 1990. Cloning and characterization of a cold-and ABA-inducible Arabidopsis gene. Plant Mol. Biol. 15: 137–144.

    Article  PubMed  CAS  Google Scholar 

  • Kurkela, S. and Borg-Franck, M. 1992. Structure and expression of kin2, one of two cold-and ABA-induced genes of Arabidopsis thaliana. Plant Mol. Biol. 19: 689–692.

    Article  PubMed  CAS  Google Scholar 

  • Kwak, J. M., Mori, I. C., Pei, Z. M., Leonhardt, N., Torres, M. A., Dangl, J. L., Bloom, R. E., Bodde, S., Jones, J. D. and Schroeder, J. I. 2003. NADPH oxidase AtrbohD and AtrbohF genes function in ROS-dependent ABA signaling in Arabidopsis. EMBO J. 22: 2623–2633.

    Article  PubMed  CAS  Google Scholar 

  • Lebel, E., Heifetz, P., Thorne, L., Uknes, S., Ryals, J. and Ward, E. 1998. Functional analysis of regulatory sequences controlling PR-1 gene expression in Arabidopsis. Plant J. 16: 223–323.

    Article  PubMed  CAS  Google Scholar 

  • Lee, Y. H. and Chun, J. Y. 1998. A new homeodomain-leucine zipper gene from Arabidopsis thaliana induced by water stress and abscisic acid treatment. Plant Mol. Biol. 37: 377–384.

    Article  PubMed  CAS  Google Scholar 

  • Leung, J., Bouvier-Durand, M., Morris, P. C., Guerrier, D., Chefdor, F. and Giraudat, J. 1994. Arabidopsis ABA response gene ABI1: features of a calcium-modulated protein phosphatase. Science 264: 1448–1452.

    Article  PubMed  CAS  Google Scholar 

  • Lippuner, V., Cyert, M. S. and Gasser, C. S. 1996. Two class of plant cDNA clones differentially complement yeast calcineurin mutants and increase salt tolerance of wild-type yeast. J. Biol. Chem. 271: 12859–12866.

    Article  PubMed  CAS  Google Scholar 

  • Liu, Q., Kasuga, M., Sakuma, Y., Abe, H., Miura, S., Yamaguchi-Shinozaki, K. and Shinozaki, K. 1998. Two transcription factors, DREB1 and DREB2, with an EREBP/ AP2 DNA binding domain separate two cellular signal transduction pathways in drought-and low-temperatureresponsive gene expression, respectively, in Arabidopsis. Plant Cell 10: 1391–1406.

    Article  PubMed  CAS  Google Scholar 

  • Medford, J. I., Elmer, J. S. and Klee, H. J. 1991. Molecular cloning and characterization of genes expressed in shoot apical meristems. Plant Cell 3: 359–370.

    Article  PubMed  CAS  Google Scholar 

  • Meyer, K., Leube, M. P. and Grill, E. 1994. A protein phosphatase 2C involved in ABA signal transduction in Arabidopsis thaliana. Science 264: 1452–455.

    Article  PubMed  CAS  Google Scholar 

  • Murata, Y., Pei, Z. M., Mori, I. C. and Schroeder, J. I. 2000. Abscisic acid activation of plasma membrane Ca2+ channels in guard cells requires cytosolic NAD(P)H and is differentially disrupted upstream and downstream of reactive oxygen species production in abi1–1 and abi2–1 protein phosphatase 2C mutants. Plant Cell 13: 2513–2523.

    Article  Google Scholar 

  • Neill, S. J., Desikan, R., Clarke and Hancock, J. T. 2002b. Hydrogen peroxide signaling. Curr. Opin. Plant Biol. 5: 388–395.

    CAS  Google Scholar 

  • Neill, S. J., Desikan, R., Clarke, A., Hurst, R. D. and Hancock, J. T. 2002a. Hydrogen peroxide and nitric oxide as signaling molecules in plants. J. Exp. Bot. 53: 1237–1247.

    Article  PubMed  CAS  Google Scholar 

  • Okamuro, J. K., Caster, B., Villarroel, V., VanMontagu, M. and Jofuku, K. D. 1997. The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis. Proc. Natl. Acad. Sci. USA 94: 7076–7081.

    Article  PubMed  CAS  Google Scholar 

  • Pei, Z. M., Murata, Y., Benning, G., Thomine, S., Klusener, B., Allen, G. J., Grill, E. and Schroeder, J. I. 2000. Calcium channels activated by hydrogen peroxide mediate abscisic acid signaling in guard cells.Nature 406: 731–734.

    Article  PubMed  CAS  Google Scholar 

  • Price, A. H. and Hendry, G. A. F. 1991. Iron-catalyzed oxygen radical formation and its possible contribution to drought damage in nine native grasses and three cereals. Plant Cell Environ. 14: 477–484.

    Article  CAS  Google Scholar 

  • Reymond, P., Weber, H., Damond, M. and Farmer, E. E. 2000. Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis. Plant Cell 12: 707–720.

    Article  PubMed  CAS  Google Scholar 

  • Rushmore, T. H., Morton, M. R. and Pickett, C. B. 1991. The antioxidant responsive element. Activation by oxidative stress and identification of the DNA consensus sequence required for functional activity. J. Biol. Chem. 266: 11632–11639.

    PubMed  CAS  Google Scholar 

  • Sakuma, Y., Liu, Q., Dubouzet, J. G., Abe, H., Shinozaki, K. and Yamaguchi-Shinozaki, K. 2002. DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration-and colinducible gene expression. Biochem. Biophys. Res. Commun. 290: 998–1009.

    Article  PubMed  CAS  Google Scholar 

  • Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

    Google Scholar 

  • Sarafian, V., Kim, Y., Poole, R. J. and Rea, P. A. 1992. Molecular cloning and sequence of cDNA encoding the pyrophosphate-energized vacuolar membrane proton pump of Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 89: 1775–1779.

    Article  PubMed  CAS  Google Scholar 

  • Seki, M., Carninci, P., Nishiyama, Y., Hayashizaki, Y. and Shinozaki, K. 1998. High-efficiency cloning of Arabidopsis full-length cDNA by biotinylated CAP trapper. Plant J. 15: 707–720.

    Article  PubMed  CAS  Google Scholar 

  • Seki, M., Ishida, J., Narusaka, M., Fujita, M., Nanjo, T., Umezawa, T., Kamiya, A., Nakajima, M., Enju, A., Sakurai, T., Satou, M., Akiyama, K., Yamaguchi-Shinozaki, K., Carninci, P., Kawai, J., Hayashizaki, Y. and Shinozaki, K. 2002c. Monitoring the expression pattern of ca. 7000 Arabidopsis genes under ABA treatments using a full-length cDNA microarray. Funct. Integr. Genomics 2: 282–291.

    Article  PubMed  CAS  Google Scholar 

  • Seki, M., Kamei, A., Yamaguchi-Shinozaki, K. and Shinozaki, K. 2003. Molecular responses to drought, salinity and frost: common and different paths for plant protection. Curr. Opin. Biotechnol. 14: 194–199.

    Article  PubMed  CAS  Google Scholar 

  • Seki, M., Narusaka, M., Abe, H., Kasuga, M., Yamaguchi-Shinozaki, K., Carninci, P., Hayashizaki, Y. and Shinozaki, K. 2001. Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses using full-length cDNA microarray. Plant Cell 13: 61–72.

    Article  PubMed  CAS  Google Scholar 

  • Seki, M., Narusaka, M., Ishida, J., Nanjo, T., Fujita, M., Oono, Y., Kamiya, A., Nakajima, M., Enju, A., Sakurai, T., Satou, M., Akiyama, K., Taji, T., Yamaguchi-Shinozaki, K., Carninci, P., Kawai, J., Hayashizaki, Y. and Shinozaki, K. 2002b. Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold, and high-salinity stresses using a full-length cDNA microarray. Plant J. 31: 279–292.

    Article  PubMed  CAS  Google Scholar 

  • Seki, M., Narusaka, M., Kamiya, A., Ishida, J., Satou, M., Sakurai, T., Nakajima, M., Enju, A., Akiyama, K., Oono, Y., Muramatsu, M., Hayashizaki, Y., Kawai, J., Carninci, P., Itoh, M., Ishii, Y., Arakawa, T., Shibata, K., Shinagawa, A. and Shinozaki, K. 2002a. Functional annotation of a fulllength Arabidopsis cDNA collection. Science 296: 141–145.

    Article  PubMed  Google Scholar 

  • Shinozaki, K. and Yamaguchi-Shinozaki, K. 1997. Gene expression and signal transduction in water-stress response. Plant Physiol. 115: 327–334.

    Article  PubMed  CAS  Google Scholar 

  • Shinozaki, K. and Yamaguchi-Shinozaki, K. 2000. Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathways. Curr. Opin. Plant Biol. 3: 217–223.

    PubMed  CAS  Google Scholar 

  • Shinozaki, K., Yamaguchi-Shinozaki, K. and Seki, M. 2003. Regulatory network of gene expression in the drought and cold stress responses. Curr. Opin. Plant Biol. 6: 410–417.

    Article  PubMed  CAS  Google Scholar 

  • Taji T., Ohsumi C., Iuchi S., Seki M., Kasuga M., Kobayashi, M., Yamaguchi-Shinozaki, K. and Shinozaki, K. 2002. Important roles of drought-and cold-inducible genes for galactinol synthase in stress tolerance in Arabidopsis thaliana. Plant J. 29: 417–426.

    Article  PubMed  CAS  Google Scholar 

  • Takahashi, S., Katagiri, T., Hirayama, T., Yamaguchi-Shinozaki, K. and Shinozaki, K. 2001. Hyperosmotic stress induces a rapid and transient increase in inositol 1, 4, 5-trisphosphate independent of abscisic acid in Arabidopsis cell culture. Plant Cell Physiol. 42: 214–222.

    Article  PubMed  CAS  Google Scholar 

  • Takahashi, S., Katagiri, T., Yamaguchi-Shinozaki, K. and Shinozaki, K. 2000. An Arabidopsis gene encoding a Ca2+-binding protein is induced by abscisic acid during dehydration. Plant Cell Physiol. 41: 898–903.

    Article  PubMed  CAS  Google Scholar 

  • Thimm, O., Essigmann, B., Kloska, S., Altmann, T. and Buckhout, T. J. 2001. Response of Arabidopsis to iron deficiency stress as revealed by microarray analysis. Plant Physiol. 127: 1030–1043.

    Article  PubMed  CAS  Google Scholar 

  • Thomashow, M. F. 1999. Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. Annu. Rev. Plant Physiol. Plant Mol. Biol. 50: 571–599.

    Article  PubMed  CAS  Google Scholar 

  • Uno, Y., Furihata, T., Abe, H., Yoshida, R., Shinozaki, K. and Yamaguchi-Shinozaki, K. 2000. Arabidopsis basic leucine zipper transcription factors involved in an abscisic aciddependent signal transduction pathway under drought and high-salinity conditions. Proc. Natl. Acad. Sci. USA 97: 11632–11637.

    Article  PubMed  CAS  Google Scholar 

  • Urao, T., Yamaguchi-Shinozaki, K., Urao, S. and Shinozaki, K. 1993. An Arabidopsis myb homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence. Plant Cell 5: 1529–1539.

    Article  PubMed  CAS  Google Scholar 

  • Xu, W., Chambbell, P., Vargheese, A. K. and Braam, J. 1996. The Arabidopsis XET-related gene family: environmental and hormonal regulation of expression. Plant J. 9: 879–889.

    Article  PubMed  CAS  Google Scholar 

  • Yamada, K., Lim, J., Dale, J. M., Chen, H., Shinn, P., Palm, C. J., Southwick, A. M., Wu, H. C., Kim, C., Nguyen, M., Pham, P., Cheuk, R., Karlin-Newmann, G., Liu, S. X., Lam, B., Sakano, H., Wu, T., Yu, G., Miranda, M., Quach, H. L., Tripp, M., Chang, C. H., Lee, J. M., Toriumi, M., Chan, M. M., Tang, C. C., Onodera, C. S., Deng, J. M., Akiyama, K., Ansari, Y., Arakawa, T., Banh, J., Banno, F., Bowser, L., Brooks, S., Carninci, P., Chao, Q., Choy, N., Enju, A., Goldsmith, A. D., Gurjal, M., Hansen, N. F., Hayashizaki, Y., Johnson-Hopson, C., Hsuan, V. W., Iida, K., Karnes, M., Khan, S., Koesema, E., Ishida, J., Jiang, P. X., Jones, T., Kawai, J., Kamiya, A., Meyers, C., Nakajima, M., Narusaka, M., Seki, M., Sakurai, T., Satou, M., Tamse, R., Vaysberg, M., Wallender, E. K., Wong, C., Yamamura, Y., Yuan, S., Shinozaki, K., Davis, R. W., Theologis, A. and Ecker, J. R. 2003. Empirical analysis of transcriptional activity in the Arabidopsis genome. Science 302: 842–846.

    Article  PubMed  CAS  Google Scholar 

  • Yamaguchi-Shinozaki, K., Koizumi, M., Urao, S. and Shinozaki, K. 1992. Molecular cloning and characterization of 9 cDNAs for genes that are responsive to desiccation in Arabidopsis thaliana: sequence analysis of one cDNA clone that encodes a putative transmembrane channel protein. Plant Cell Physiol. 33: 217–224.

    CAS  Google Scholar 

  • Yamaguchi-Shinozaki, K. and Shinozaki, K. 1994. A novel cisacting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell 6: 251–264.

    Article  PubMed  CAS  Google Scholar 

  • Yuasa, T., Ichimura, K., Mizoguchi, T. and Shinozaki, K. 2001. Oxidative stress activates ATMPK6, an Arabidopsis homologue of MAP kinase. Plant Cell Physiol. 42: 1012–1016.

    Article  PubMed  CAS  Google Scholar 

  • Zhu, J.-K. 2002. Salt and drought stress signal transduction in plants. Annu. Rev. Plant Biol. 53: 247–273.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, 305-0074, Japan

    Seiji Takahashi, Motoaki Seki & Kazuo Shinozaki

  2. Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Japan, 230-0045

    Motoaki Seki, Junko Ishida, Masakazu Satou, Tetsuya Sakurai, Mari Narusaka, Asako Kamiya, Maiko Nakajima, Akiko Enju, Kenji Akiyama & Kazuo Shinozaki

  3. Genomic Knowledge Base Research Team, Bioinformatics Group, RIKEN Genomic Science Centre, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan

    Masakazu Satou, Tetsuya Sakurai & Kenji Akiyama

  4. Biological Resources Division, Japan International Research Center for Agricultural Sciences, Ministry of Agriculture, Forestry, and Fisheries, 2-1 Ohwashi, Tsukuba, Ibaraki, 305-0851, Japan

    Kazuko Yamaguchi-Shinozaki

Authors
  1. Seiji Takahashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Motoaki Seki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Junko Ishida
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Masakazu Satou
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tetsuya Sakurai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mari Narusaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Asako Kamiya
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Maiko Nakajima
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Akiko Enju
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Kenji Akiyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Kazuko Yamaguchi-Shinozaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Kazuo Shinozaki
    View author publications

    You can also search for this author in PubMed Google Scholar

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Takahashi, S., Seki, M., Ishida, J. et al. Monitoring the expression profiles of genes induced by hyperosmotic, high salinity, and oxidative stress and abscisic acid treatment in Arabidopsis cell culture using a full-length cDNA microarray. Plant Mol Biol 56, 29–55 (2004). https://doi.org/10.1007/s11103-004-2200-0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11103-004-2200-0

Search

Navigation