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Cloning of cDNAs for genes that are early-responsive to dehydration stress (ERDs) inArabidopsis thaliana L.: identification of three ERDs as HSP cognate genes

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Abstract

InArabidopsis thaliana L., accumulation of abscisic acid (ABA) began to increase 2 h after plants had been subjected to dehydration stress and reached maximum levels after 10h. Differential hybridization was used to isolate 26Arabidopsis cDNAs with gene expression induced by a 1 h dehydration treatment. The cDNA clones were classified into 16 groups based on Southern blot hybridization, and named ERD (early-responsive todehydration) clones. Partial sequencing of the cDNA clones revealed that three ERDs were identical to those of HSP cognates (Athsp70-1, Athsp81-2, and ubiquitin extension protein). Dehydration stress strongly induced the expression of genes for the three ERDs, while application of ABA, which is known to act as a signal transmitter in dehydration-stressed plants, did not significantly affect the ERD gene expression. This result suggests that these HSP cognates are preferentially responsive to dehydration stress inA. thaliana, and that signaling pathways for the expression of these genes under conditions of dehydration stress are not mainly mediated by ABA. We also discuss the possible functions of these three ERD gene products against dehydration stress.

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Authors and Affiliations

  1. Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research (RIKEN), Tsukuba Life Science Center, 3-1-1 Koyadai, 305, Tsukuba, Ibaraki, Japan

    Tomohiro Kiyosue, Kazuko Yamaguchi-Shinozaki & Kazuo Shinozaki

  2. Biological Resources Division, Japan International Research Center for Agricultural Science (JIRCAS), Ministry of Agriculture, Forestry and Fisheries, 1-2 Oowashi, 305, Tsukuba, Ibaraki, Japan

    Kazuko Yamaguchi-Shinozaki

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  1. Tomohiro Kiyosue
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  2. Kazuko Yamaguchi-Shinozaki
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Kiyosue, T., Yamaguchi-Shinozaki, K. & Shinozaki, K. Cloning of cDNAs for genes that are early-responsive to dehydration stress (ERDs) inArabidopsis thaliana L.: identification of three ERDs as HSP cognate genes. Plant Mol Biol 25, 791–798 (1994). https://doi.org/10.1007/BF00028874

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