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Photosynthetic characteristics of diploid honeysuckle (Lonicera japonica Thunb.) and its autotetraploid cultivar subjected to elevated ozone exposure

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Photosynthetica

Abstract

In order to investigate the effect of chromosome doubling on ozone tolerance, we compared the physiological responses of a diploid honeysuckle (Lonicera japonica Thunb.) and its autotetraploid cultivar to elevated ozone (O3) exposure (70 ng g−1, 7 h d−1 for 31 d). Net photosynthetic rate (P N) of both cultivars were drastically (P<0.01) impaired by O3. Although there were significantly positive correlation between P N and stomatal conductance (g s) in both cultivars under each treatment, the decreased g s in O3 might be the result rather than the cause of decreased P N as indicated by stable or increasing the ratio of intercellular to ambient CO2 concentration(C i/C a). P N under saturating CO2 concentration (P Nsat) and carboxylation efficiency (CE) significantly decreased under O3 fumigation, which indicated the Calvin cycle was impaired. O3 also inhibited the maximum efficiency of photosystem II (PSII) photochemistry in the dark-adapted state (Fv/Fm), actual quantum yield of PSII photochemistry (ΦPSII), electron transport rate (ETR), photochemical quenching coefficient (qP), non-photochemical quenching (NPQ), the maximum in vivo rate of Rubisco carboxylation (Vcmax) and the maximal photosynthetic electron transport rate (Jmax) which demonstrated that the decrease in P N of the honeysuckle exposed to elevated O3 was probably not only due to impairment of Calvin cycle but also with respect to the light-harvesting and electron transport processes. Compared to the diploid, the tetraploid had higher relative loss in transpiration rate (E), (g s), (P Nsat), Vcmax and Jmax. This result indicated that the Calvin cycle and electron transport in tetraploid was damaged more seriously than in diploid. A barely nonsignificant (P=0.086) interaction between O3 and cultivar on P N suggested a higher photosynthetic sensitivity of the tetraploid cultivar.

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Abbreviations

CE:

carboxylation efficiency

Chl:

chlorophyll

C i :

intercellular CO2 concentration

Ci/Ca:

the ratio of intercellular to ambient CO2 concentration

E :

transpiration rate

ETR:

electron transport rate

Fv/Fm :

maximum efficiency of photosystem II photochemistry in the dark-adapted state

g s :

stomatal conductance

Jmax :

the maximal photosynthetic electron transport rate

NPQ:

non-photochemical quenching

PAR:

photosynthetically active radiation

P N :

net photosynthetic rate

P Nsat :

PN under saturating CO2 concentration

PSII:

photosystem II

ΦPSII :

actual quantum yield of PSII photochemistry

qp :

photochemical quenching coefficient

Vcmax :

the maximum in vivo rate of Rubisco carboxylation

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Acknowledgments

The authors thank Mr. Chen Shengbin in Chinese Academy of Sciences and Mr. Su Benying in Shandong Agricultural University for their technical help during the experiment. We thank Dr. Xiuming Hao in Greenhouse and Processing Crops Research Centre, Agriculture and Agri-Food Canada for his good suggestions to improve the manuscript. This investigation was funded by the BNSF (07d0413), BNSF (8062017) and NSFC (30900200).

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Authors and Affiliations

  1. State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, 100093, Beijing, P.R. China

    L. Zhang, H. Xu, G. -M. Jiang & Y. -G. Li

  2. Chinese Academy of Sciences, Graduate University, 100049, Beijing, P.R. China

    L. Zhang

  3. Beijing Museum of Natural History, 100050, Beijing, P. R. China

    J. -C. Yang

  4. School of Chinese Pharmacy, Beijing University of Chinese Medicine, 100102, Beijing, P. R. China

    W. -D. Li

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  1. L. Zhang
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  2. H. Xu
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Correspondence to Y. -G. Li.

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Zhang, L., Xu, H., Yang, J.C. et al. Photosynthetic characteristics of diploid honeysuckle (Lonicera japonica Thunb.) and its autotetraploid cultivar subjected to elevated ozone exposure. Photosynthetica 48, 87–95 (2010). https://doi.org/10.1007/s11099-010-0012-9

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