Abstract
Purpose
To compare the ability of alkyl-aryl isothiocyanates (ITCs) to increase the activities of the Phase 2 detoxification enzymes NAD[P]H:quinone acceptor oxidoreductase 1 (NQO1) and glutathione S-transferases (GST) in rat tissues in vivo and in cells in vitro.
Materials and Methods
Twelve alkyl-aryl ITCs and the fully-reduced derivative of benzyl ITC (cyclohexylmethyl ITC) were administered to rats each day for 5 days. The animals were then killed and organs harvested. The ITCs were also evaluated in a bladder cell line in culture. The activities of NQO1 and GST in the organs and cells were measured.
Results
In vivo, the organ most susceptible to the inductive activity of the ITCs was the urinary bladder, with α-methylbenzyl ITC and cyclohexylmethyl ITC being the most effective. Inductive activity in the bladder in vivo did not, however, correlate with that in bladder cells in vitro.
Conclusions
Induction of Phase 2 enzymes increases resistance to chemical carcinogenesis. ITCs could therefore be valuable chemopreventative agents, and the specificity of these substances toward the urinary bladder suggest that they could be particularly useful for protecting against bladder cancer. In this regard, α-methylbenzyl ITC and cyclohexylmethyl ITC could be especially valuable.
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Acknowledgements
This work was funded by the Waikato Medical Research Foundation (New Zealand) and National Cancer Institute Grant CA 80962 (USA).
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Munday, R., Zhang, Y., Munday, C.M. et al. Structure–Activity Relationships and Organ Specificity in the Induction of GST and NQO1 by Alkyl-Aryl Isothiocyanates. Pharm Res 25, 2164–2170 (2008). https://doi.org/10.1007/s11095-008-9595-2
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DOI: https://doi.org/10.1007/s11095-008-9595-2