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Down-regulation of Pin1 in Temporal Lobe Epilepsy Patients and Mouse Model

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Abstract

Peptidyl-prolyl cis–trans isomerase NIMA-interacting 1 (Pin1) is a unique PPIase belonging to the parvulin family, and it isomerizes peptide bond between phospho-(Ser/Thr) and Pro. Pin1 has been linked to the pathogenesis of various human diseases; however, its exact biological functions remain unclear. The aim of the present study is to explore the expression pattern of Pin1 in patients with refractory epilepsy and in a chronic pilocarpine-induced epileptic mouse model. Using Western blot, immunofluorescence and immunoprecipitation analysis, we found that Pin1 protein was mainly distributed in neurons, demonstrated by colocalization with the dendritic marker, MAP2. However, the expression of Pin1 decreased remarkably in epileptic patients and experimental mice. Furthermore, the reciprocal coimmunoprecipitation analysis showed that Pin1 interacted with NR2A and NR2B-containing NMDA receptors not AMPA receptors in epileptic mouse models. Our results are the first to indicate that the expression of Pin1 in epileptic brain tissue could play important roles in epilepsy.

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Acknowledgements

This study was supported by grants from the National Natural Science Foundation of China (No. 31500821) and the National Clinical Key Specialty Construction Foundation of China. We are grateful for the support of The Affiliated Children’s Hospital of Capital Institute of Pediatrics, The First Affiliated Hospital of PLA General Hospital and The First Affiliated Hospital of Chongqing Medical University, which supplied patient brain tissues. We also thank all patients and their families for their participation in this study.

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Authors and Affiliations

  1. The Public Health Center, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Chongqing, 400016, China

    Lan Tang

  2. Department of Neurology, Chongqing Key Laboratory of Neurology, The First Affiliated Hospital of Chongqing Medical University, 1 Youyi Road, Chongqing, 400016, China

    Yanke Zhang, Guojun Chen, Xuefeng Wang & Binglin Zhu

  3. Department of Neurology, The People’s Hospital of Yubei District of Chongqing City, 62 Jianshe Road, Chongqing, 401120, China

    Yan Xiong

  4. Center of Epilepsy, Beijing Institute for Brain Disorders, 10 Xitoutiao, Youanmen, Fengtai District, Beijing, 100069, China

    Xuefeng Wang

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  1. Lan Tang
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  2. Yanke Zhang
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  3. Guojun Chen
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  4. Yan Xiong
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Correspondence to Xuefeng Wang or Binglin Zhu.

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Tang, L., Zhang, Y., Chen, G. et al. Down-regulation of Pin1 in Temporal Lobe Epilepsy Patients and Mouse Model. Neurochem Res 42, 1211–1218 (2017). https://doi.org/10.1007/s11064-016-2158-8

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  • DOI: https://doi.org/10.1007/s11064-016-2158-8

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