Abstract
Epilepsy is a common neurological disorder. Because its underlying mechanisms remain incompletely understood, current treatments are not adequate for all epilepsy patients, and some patients progress to refractory epilepsy. Under physiological conditions, excitatory and inhibitory neurons function in a dynamic balance. Epilepsy develops when this balance is disrupted. Intersectin1-L is a major scaffold protein in the central nervous system that contains multiple functional domains, and it is the long form of intersectin1. Recent studies have shown that intersectin1-L plays an important role in the process of neurotransmitter release. In this study, we investigated the expression pattern and distribution of intersectin1-L in patients with refractory epilepsy, in a rat model of pilocarpine-induced epilepsy, and in a rat model of amygdala-kindled epilepsy by immunohistochemistry, immunofluorescence, and Western blotting. The purpose of this study was to explore the relationship between epilepsy and intersectin1-L. The results showed that the intersectin1-L protein was primarily expressed in neurons in brain tissue. Its expression was remarkably increased in patients with refractory epilepsy and in epilepsy model rats. These results suggest that the abnormal expression of the intersectin1-L protein in epileptic brain tissue may play an important role in epilepsy, especially refractory epilepsy.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (Nos. 81271445 and 81301110). We would like to acknowledge Xuanwu Hospital, Capital Medical University, Tiantan Hospital, the Third Military Medical University, Xinqiao Hospital and the First Affiliated Hospital of Chongqing Medical University, which provided the brain tissue samples. We also thank the patients and their families who participated in this study.
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All authors report no conflicts of interest.
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This study was approved by the Ethics Committee of Chongqing Medical University. The entire study protocol was in compliance with the Declaration of Helsinki of the World Medical Association guidelines. All efforts were made to minimize the number of animals used and their suffering.
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Xiaoyan Yang and Xin Xu have contributed equally to this work.
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Yang, X., Xu, X., Zhang, Y. et al. Altered Expression of Intersectin1-L in Patients with Refractory Epilepsy and in Experimental Epileptic Rats. Cell Mol Neurobiol 35, 871–880 (2015). https://doi.org/10.1007/s10571-015-0181-y
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DOI: https://doi.org/10.1007/s10571-015-0181-y