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Paracoccidioides brasiliensis-associated dermatitis and lymphadenitis in a dog

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Abstract

Paracoccidioidomycosis (PCM) is an endemic disease of humans from Latin America that is caused by Paracoccidioides brasiliensis and P. lutzii, with most cases of PCM in domestic animals being associated with P. brasiliensis. This study presents the clinical, cytological, mycological, serological, and molecular findings associated with P. brasiliensis in a dog from Southern Brazil. Fine needle biopsies were collected from the skin and several lymph nodes of a 5-year-old female Labrador dog that had enlargement of most superficial lymph nodes. Cytology of the skin and lymph nodes revealed pyogranulomatous dermatitis and lymphadenitis associated with fine-necked, budding fungal structures consistent with the Paracoccidioides genus of organisms; mycological culture derived from the lymph node aspirate demonstrated similar budding structures. Serological assays using exoantigens obtained from the fungal culture demonstrated that the fungal organisms derived from the lymph node were antigenically similar to P. brasiliensis by immunodiffusion and Western blot. A PCR assay, using the fungal culture as input, amplified a partial segment of the internal transcribed spacer 1 and 2 regions of P. brasiliensis; direct sequencing and phylogenetic analyses confirmed the PCR product as P. brasiliensis. The combined cytological, mycological, serological, and molecular findings confirmed a diagnosis of fungal dermatitis and lymphadenitis due to P. brasiliensis in this dog. This case represents the third description of clinical PCM in dogs and the first confirmation of mycotic dermatitis associated with P. brasiliensis in this species. The participation of dogs in the possible dissemination of PCM is reviewed, and it is proposed that dogs are probable accidental hosts in the epidemiological cycle associated with P. brasiliensis.

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Acknowledgments

Selwyn A. Headley, Amauri A. Alfieri, and Mario Augusto Ono are recipients of the National Council for Scientific and Technological Development (CNPq; Brazil) fellowships and grants. The authors thank the owners of this dog for the usage during this investigation. We express sincere appreciation and gratitude to Mrs Eliana Celia Pereira, technician Laboratory of Animal Mycology, Universidade Estadual de Londrina, for the preparation of the fungal culture used during this investigation.

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Authors and Affiliations

  1. Laboratory of Animal Pathology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445 km 380, Campus Universitário, PO Box 10.011, Londrina, Paraná, 86057-970, Brazil

    Selwyn Arlington Headley, Giovana Wingeter Di Santis & Daniela Farias da Nóbrega

  2. Multi-User Animal Health Laboratory, Organic Tissue Processing Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil

    Selwyn Arlington Headley & Giovana Wingeter Di Santis

  3. Laboratory of Mycology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil

    Lucienne Garcia Pretto-Giordano

  4. Small Animal Internal Medicine, Department of Veterinary Clinics, Universidade Estadual de Londrina, Londrina, Paraná, Brazil

    Lucas Alécio Gomes & Katherine Moura Leite

  5. Laboratory of Applied Immunology, Department of Pathological Sciences, Universidade Estadual de Londrina, Londrina, Paraná, Brazil

    Mario Augusto Ono

  6. Laboratory of Molecular Biology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil

    Brígida Kussumoto de Alcântara & Amauri Alcindo Alfieri

  7. Laboratory of Applied Immunology, Department of Pathological Sciences, Universidade Estadual de Londrina, Londrina, Paraná, Brazil

    Eiko Nakagawa Itano

  8. Multi-User Animal Health Laboratory, Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, Paraná, Brazil

    Rafaela Macagnan & Amauri Alcindo Alfieri

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  1. Selwyn Arlington Headley
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  2. Lucienne Garcia Pretto-Giordano
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Correspondence to Selwyn Arlington Headley.

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Headley, S.A., Pretto-Giordano, L.G., Di Santis, G.W. et al. Paracoccidioides brasiliensis-associated dermatitis and lymphadenitis in a dog. Mycopathologia 182, 425–434 (2017). https://doi.org/10.1007/s11046-016-0075-7

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  • DOI: https://doi.org/10.1007/s11046-016-0075-7

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