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Occurrence of Antibodies to Paracoccidioides brasiliensis in Dairy Cattle from Mato Grosso do Sul, Brazil

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Abstract

The aim of this study was to evaluate the humoral immune response in cattle immunized with Paracoccidioides brasiliensis and perform a seroepidemiological study of paracoccidioidomycosis in dairy cattle from Mato Grosso do Sul. Two animals (one steer and one heifer) were inoculated with a suspension of P. brasiliensis in Freund incomplete adjuvant. Blood samples were collected periodically to evaluate humoral immune response by immunodiffusion and ELISA, using exoantigen and gp43 as antigens, respectively. The antibody production was detected by immunodiffusion and ELISA, in both animals, 14 days after immunization. The soroepidemiologic study was carried out in 400 cattle of Mato Grosso do Sul from four municipalities: Corumbá, Dourados, Nova Andradina, and São Gabriel d’Oeste. The municipalities of Corumbá (30%) and Nova Andradina (28%) showed higher positivity than Dourados (8%) and São Gabriel d’Oeste (4%). In this study we concluded that cattle immunized with P. brasiliensis develop humoral immune response for gp43, remaining with high titers of antibodies, and that this animal species could be an epidemiologic marker of paracoccidioidomycosis.

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Acknowledgments

The authors thank CNPq, CAPES, and Araucaria Foundation for financial support and Dr. Roberta Lemos Freire for aid with statistical analysis.

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Authors and Affiliations

  1. Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Campus Universitário, Londrina, PR, 86051-980, Brazil

    L. H. Silveira, E. N. Itano & M. A. Ono

  2. Faculdades Luiz Meneghel, Bandeirantes, PR, Brazil

    L. H. Silveira & E. V. Medeiros

  3. Laboratório de Sanidade Animal, Agência Estadual de Defesa Sanitária Animal e Vegetal do Mato Grosso do Sul, IAGRO, Campo Grande, MS, Brazil

    R. C. S. Paes

  4. Disciplina de Biologia Celular, Universidade Federal de São Paulo, Sao Paulo, SP, Brazil

    Z. P. Camargo

Authors
  1. L. H. Silveira
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  2. R. C. S. Paes
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  3. E. V. Medeiros
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  4. E. N. Itano
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  5. Z. P. Camargo
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  6. M. A. Ono
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Correspondence to M. A. Ono.

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Silveira, L.H., Paes, R.C.S., Medeiros, E.V. et al. Occurrence of Antibodies to Paracoccidioides brasiliensis in Dairy Cattle from Mato Grosso do Sul, Brazil. Mycopathologia 165, 367–371 (2008). https://doi.org/10.1007/s11046-008-9095-2

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  • DOI: https://doi.org/10.1007/s11046-008-9095-2

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