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Evaluation of Paracoccidioides brasiliensis Infection in Dairy Goats

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Abstract

Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, a systemic mycosis that affects mainly rural workers in Brazil and other Latin American countries. The participation of domestic and wild animal species in the ecoepidemiology of paracoccidioidomycosis is not well understood. The objective of this study was to evaluate P. brasiliensis infection in dairy goats. The humoral immune response to the gp43 antigen, the main antigen used for paracoccidioidomycosis serodiagnosis and seroepidemiology, was evaluated in two goats immunized with inactivated P. brasiliensis yeast cells. Both animals produced antibodies against the P. brasiliensis gp43 antigen, detected by ELISA, 2 weeks after immunization. A total of 202 goat serum samples were analyzed by ELISA and the immunodiffusion test using P. brasiliensis gp43 and exoantigen as antigens. The seropositivity observed by ELISA was 26.2 % although no reactivity was detected by immunodiffusion. The animals over 18 months of age showed significantly higher positivity (40 %) than animals aged 6–18 months (14.8 %) and 0–6 months (2.6 %). Taking into account that cross-reactivity may occur with other pathogens, the serum samples were also analyzed by ELISA using Histoplasma capsulatum exoantigen as antigen and the positivity observed was 14.3 %. The low correlation (0.267) observed between reactivity to P. brasiliensis gp43 and H. capsulatum exoantigen suggests co-infection rather than cross-reactivity. This is the first report showing serological evidence of P. brasiliensis infection in goats and reinforces that domestic animals are useful epidemiological markers of paracoccidioidomycosis.

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Acknowledgments

The authors thank the CNPq, CAPES and the Araucaria Foundation for financial support and the CNPq for the productivity fellowship granted to MA Ono, IT Navarro, EN Itano and ZP Camargo.

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Authors and Affiliations

  1. Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Campus Universitário, Londrina, Paraná, 86057-970, Brazil

    Jacqueline B. Ferreira, Gabriela G. Oliveira, Aline M. Omori, Donizeti R. Belitardo, Eiko N. Itano & Mario A. Ono

  2. Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, Paraná, Brazil

    Italmar T. Navarro & Roberta L. Freire

  3. Disciplina de Biologia Celular, Universidade Federal de São Paulo, São Paulo, Brazil

    Zoilo P. Camargo

Authors
  1. Jacqueline B. Ferreira
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  2. Italmar T. Navarro
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  3. Roberta L. Freire
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  4. Gabriela G. Oliveira
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  5. Aline M. Omori
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  6. Donizeti R. Belitardo
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  7. Eiko N. Itano
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  8. Zoilo P. Camargo
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  9. Mario A. Ono
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Correspondence to Mario A. Ono.

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Ferreira, J.B., Navarro, I.T., Freire, R.L. et al. Evaluation of Paracoccidioides brasiliensis Infection in Dairy Goats. Mycopathologia 176, 95–99 (2013). https://doi.org/10.1007/s11046-013-9644-1

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  • DOI: https://doi.org/10.1007/s11046-013-9644-1

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