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LncRNA MEG3 induces endothelial differentiation of mouse derived adipose-derived stem cells by targeting MiR-145-5p/KLF4

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Abstract

Background

The present study aimed to investigate the mechanisms through which long non-coding RNA (lncRNA) maternally expressed 3 (MEG3) affected the endothelial differentiation of mouse derived adipose-derived stem cells (ADSCs).

Materials and methods

ADSCs were isolated and identified by specific surface marker detection. The effects of lncRNA MEG3 on endothelial differentiation of ADSCs were also detected via quantitative PCR, western blotting, immunofluorescence and Matrigel angiogenesis assays. In addition, using target gene prediction tools and luciferase reporter assays, the downstream target gene was demonstrated.

Results

LncRNA MEG3 targeted and reduced the expression levels of microRNA-145-5p (miR-145-5p), which upregulated the expression levels of Krüppel like factor 4 (KLF4), promoting endothelial differentiation of ADSCs.

Conclusion

LncRNA MEG3 induced endothelial differentiation of ADSCs by targeting miR-145-5p/KLF4, which may provide novel insights to illustrate the mechanism of endothelial differentiation of ADSCs.

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Data availability

All data generated or analysed during this study are included in this published article.

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Acknowledgements

The authors would like to thank Nanjing Berke Biology Co., Ltd. provided technical support of adipose-derived stem cell separation technology.

Funding

The present study was supported by the Traditional Chinese Medicine Research Project of Heilongjiang (Grant Nos. ZHY2020-041 and ZHY19-062).

Author information

Authors and Affiliations

  1. Department of Dermatology, First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Harbin, 150006, Heilongjiang, People’s Republic of China

    Hailong Zhang

  2. Department of Medicine, Second Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Harbin, 150006, Heilongjiang, People’s Republic of China

    Gang Liu

  3. Department of Health Center, Heilongjiang University of Traditional Chinese Medicine, Harbin, 150040, Heilongjiang, People’s Republic of China

    Xu Mao

  4. Department of Medicine, Heilongjiang Academy of Traditional Chinese Medicine, No. 33 of West Dazhi Street, Harbin, 150001, Heilongjiang, People’s Republic of China

    Lei Yang, Bingyu Wang & Xingxing Yuan

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  1. Hailong Zhang
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  2. Gang Liu
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  3. Xu Mao
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  4. Lei Yang
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Contributions

HZ conceived and designed the analysis. GL, BW and LY performed the experiments. XM and XY analyzed and interpreted the data. XY drafted the manuscript. HZ confirm the authenticity of all the raw data. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Bingyu Wang or Xingxing Yuan.

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Conflict of interest

The authors declare that they have no competing interests.

Ethics approval and consent to participate

All operations were approved by the Ethics Research Committee of the Heilongjiang Academy of Traditional Chinese Medicine (permit no. SY3R-2019004; Harbin, China).

Patient consent for publication

Not applicable.

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Supplementary Information

Below is the link to the electronic supplementary material.

11033_2022_7671_MOESM1_ESM.tif

S1. Characterization of ADSCs. ADSCs were isolated from the adipose tissues. At the third passage, the expression levels of ADSCs markers (A) CD44 and CD90, and (B) CD31 and HLA-DR were analyzed via flow cytometry. ADSCs, adipose-derived stem cells; HLA-DR, major histocompatibility complex, class II antigen-associated. Supplementary file1 (TIF 4791 kb)

11033_2022_7671_MOESM2_ESM.tif

S2. Transfection efficiency in ADSCs. ADSCs were transfected with (A) sh-MEG3, (B) miR-145-5p mimic, (C) miR-145-5p inhibitor, (D) sh-KLF4 and (E) pc-MEG3 and qRT-PCR were conducted to measure the transfection efficiency 36 h post-transfection. Supplementary file2 (TIF 2081 kb)

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Zhang, H., Liu, G., Mao, X. et al. LncRNA MEG3 induces endothelial differentiation of mouse derived adipose-derived stem cells by targeting MiR-145-5p/KLF4. Mol Biol Rep 49, 8495–8505 (2022). https://doi.org/10.1007/s11033-022-07671-z

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  • DOI: https://doi.org/10.1007/s11033-022-07671-z

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