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Genome-wide identification and characterization of Hsp70 gene family in Nicotiana tabacum

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Abstract

Heat shock proteins 70 (Hsp70) constitute a highly conserved protein family of cellular chaperones widely distributed in plants, where they play a fundamental role in response to biotic and abiotic stress. Until now, genome-wide analyses of the Hsp70 gene family have been conducted for some species. However, reports about Hsp70 genes in Nicotiana tabacum are scarce. In this study, we systematically conducted genome-wide identification and expression analysis of the Hsp70 gene family in tobacco, including gene structure, classification, evolutionary relationships, promoters, and transcript levels in response to abiotic stress treatments. In all, 61 Hsp70 members were identified and classified into six groups that were mapped onto 18 chromosomes, where most were distributed on both ends of the chromosome. The conserved structures and motifs of NtHsp70 proteins in the same subfamily were highly consistent. At least 15 pairs of NtHsp70 genes underwent gene duplication by segment and tandem duplications. Most NtHsp70 proteins contained N-terminal hexokinase conserved motifs. Phylogenetic analysis showed that most species expanded according to their own species-specific approach during the evolution of Hsp70s. Tissue-specific expression analysis indicated that all NtHsp70 genes were involved in at least one or more abiotic stress responses, highlighting the wide participation of NtHsp70 genes in environmental adaptation. This is the first genome-wide analysis of Hsp70 in N. tabacum. These results indicate that each NtHsp70 member fulfilled distinct functions in response to various abiotic stresses.

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Data availability

The data sets supporting the results of this article are included within the article and its additional file.

Abbreviations

Hsp70:

Heat shock protein 70

NtHsp70 :

Gene in Nicotiana tabacum

ABA:

Abscisic acid

PEG:

Polyethylene glycol

qRT-PCR:

Quantitative real-time PCR

Chlo:

Chloroplast

Cyto:

Cytosol

Endo:

Endoplasmic reticulum

Mito:

Mitochondria

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Acknowledgements

We thank China National Tobacco Corp., Chongqing Division and Henan Agricultural University for financial support and technical assistance. We also appreciate the reviewers and editors for the patience to the work.

Funding

This work was supported by China National Tobacco Corp., Yunnan Division (2016YN10, 2017YN19) and China National Tobacco Corp., Chongqing Division (NY20180601070002).

Author information

Authors and Affiliations

  1. Key Laboratory for Cultivation of Tobacco Industry, College of Tobacco Science, Henan Agricultural University, Zhengzhou, China

    Zhaopeng Song, Feilong Pan & Hongying Zhang

  2. China Tobacco Zhejiang Industrial Co., Ltd, Hangzhou, China

    Xiaoping Lou & Baoquan Zhang

  3. Chongqing Tobacco Science Research Institute, Chongqing, China

    Daibin Wang & Chao Yang

Authors
  1. Zhaopeng Song
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  6. Baoquan Zhang
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  7. Hongying Zhang
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Contributions

ZPS and FLP conceived and designed the experiments. XPL and DBW performed the experiments and participated to the data analysis. CY and BQZ performed the qRT-PCR experiments. HYZ revised the manuscript.

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Correspondence to Hongying Zhang.

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Song, Z., Pan, F., Lou, X. et al. Genome-wide identification and characterization of Hsp70 gene family in Nicotiana tabacum. Mol Biol Rep 46, 1941–1954 (2019). https://doi.org/10.1007/s11033-019-04644-7

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