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Functional analysis of Hsp70 superfamily proteins of rice (Oryza sativa)

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Cell Stress and Chaperones Aims and scope

Abstract

Heat stress results in misfolding and aggregation of cellular proteins. Heat shock proteins (Hsp) enable the cells to maintain proper folding of proteins, both in unstressed as well as stressed conditions. Hsp70 genes encode for a group of highly conserved chaperone proteins across the living systems encompassing bacteria, plants, and animals. In the cellular chaperone network, Hsp70 family proteins interconnect other chaperones and play a dominant role in various cell processes. To assess the functionality of rice Hsp70 genes, rice genome database was analyzed. Rice genome contains 32 Hsp70 genes. Rice Hsp70 superfamily genes are represented by 24 Hsp70 family and 8 Hsp110 family members. Promoter and transcript expression analysis divulges that Hsp70 superfamily genes plays important role in heat stress. Ssc1 (mitochondrial Hsp70 protein in yeast) deleted yeast show compromised growth at 37 °C. Three mitochondrial rice Hsp70 sequences (i.e., mtHsp70-1, mtHsp70-2, and mtHsp70-3) complemented the Ssc1 mutation of yeast to differential extents. The information presented in this study provides detailed understanding of the Hsp70 protein family of rice, the crop species that is the major food for the world population.

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Acknowledgments

We thank Thomas Langer, University of Cologne, Germany, for providing us yeast mutants of Hsp70. We thank the financial support from the Centre for Plant Molecular Biology and Indo-Finland project from the Department of Biotechnology, Government of India.

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Authors and Affiliations

  1. Department of Plant Molecular Biology, University of Delhi South Campus, N Delhi, 110021, India

    Neelam K. Sarkar, Preeti Kundnani & Anil Grover

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  1. Neelam K. Sarkar
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  2. Preeti Kundnani
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  3. Anil Grover
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Correspondence to Anil Grover.

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Supplementary Fig. 1

Tandem arrangement of Hsp70 genes on chromosome11. (PPTX 337 kb)

Supplementary Fig. 2

Phylogenetic relation of rice Hsp70 genes with yeast and plant Hsp70 genes. Amino acid sequences of rice and other plant Hsp70 genes aligned in ClustalX (2.0) were used for generating bootstrap (1,000 iterations) NJ tree. The tree was visualized in Treeview 1.6.6. Pink-and blue-colored fonts represent rice and yeast Hsp70 genes, respectively and black font denotes other plant species. The abbreviations used are: Os Oryza sativa, At Arabidopsis thaliana, Cr Chlamydomonas reinhardtii, Sb Sorghum bicolor, Vv Vitis vinifera, Rc Ricinus communis, Bd Brachypodium distachyon, Pt Populus trichocarpa. The accession numbers are: CrHsp70G-XP_001690543.1, Bd1g69700-XP_003558484.1, VvHsp70-CAN68225.1, RcHsp70-XP_002516783.1, Pt081540-XP_002311619.1, Pt10s09880-XP_002315776.1, Sb42680-XP_002465635.1, Bd4g39820-XP_003576944.1, Bd3562113-XP_003562113.1, CrHsp70B-XP_001696432.1, BdHsc70-XP_003561778.1, Sb39510-XP_002468096.1, Sb39500-XP_002468095.1, Sb39390-XP_002465468.1, CrBip1-XP_001701685.1, CrBip2-XP_001701884.1 (JPEG 625 kb)

Supplementary Fig. 3

Microarray based expression meta-analysis of Hsp70 genes during development stages of rice plant (a) and in various tissues (b). Microarray-based analysis was performed in silico at rice oligonucleotide array database (www.ricearray.org/). (PPTX 2377 kb)

Supplementary Table 1

List of primers used in this study. (XLSX 9 kb)

Supplementary Table 2

Predictions of cellular localization of Hsp70 genes. (XLSX 13 kb)

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Sarkar, N.K., Kundnani, P. & Grover, A. Functional analysis of Hsp70 superfamily proteins of rice (Oryza sativa). Cell Stress and Chaperones 18, 427–437 (2013). https://doi.org/10.1007/s12192-012-0395-6

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