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Polymorphisms in XPC gene and risk for prostate cancer

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Abstract

Single nucleotide polymorphisms (SNP) in repair gene DNA such as XPC gene can reduce the DNA repair capacity (DRC). Reduced DRC induce genetic instability and may increase the susceptibility to prostate cancer (PC). We conducted a case-controls study to examine the relationship between XPC Lys939Gln and XPC-PAT polymorphisms and the risk for prostate cancer in Tunisian population. We have also correlated molecular results with clinical parameters (Gleason score and TNM status) and lifestyle factors (tobacco status, alcohol consumption, and exposition to professional risk factors) of prostate cancer patients. We have found that the XPC Lys939Gln polymorphism was not associated with a risk of prostate cancer. However the XPC PAT I/I genotype was found to be associated with 3.83-fold increased risk of prostate cancer compared to controls (p = 0.00006; OR 3.83; 95% CI (1.83–8.05)). The test of linkage disequilibrium showed that XPC-PAT polymorphism is in linkage disequilibrium with XPC Lys939Gln variants. The combined analysis of XPC Lys939Gln and XPC-PAT variants showed that patients who inherited (Lys/Gln + PAT D/D) genotypes were protected against prostate cancer development compared to controls. In the other hand, no significant association has been found between XPC polymorphisms and clinical parameters or between XPC polymorphisms and lifestyle factors.

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Data availability

Data sharing not applicable to this article as no datasets were generated or analyzed during the current study.

Abbreviations

SNP:

Single nucleotide polymorphisms

DRC:

DNA repair capacity

PC:

Prostate cancer

PSA:

Prostate-specific antigen

NER:

Nucleotide excision repair

BER:

Base excision repair

MMR:

Mismatch repair

DSBR:

Double-strand break repair

TCR:

Transcription-coupled repair

XPC:

Xeroderma pigmentosum complementary group C

DRE:

Digital rectal examination

EDTA:

Ethylene diamine tetra-acetic acid

PCR:

Polymerase chain reaction

PCR-RFLP:

Restriction Fragment Length polymorphism

ORs:

Odds ratios

CI:

Confidence intervals

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Acknowledgements

The team work would like to express their thanks and gratitude to the medical team of Urology department, Charles Nicolle Hospital, Tunis—Tunisia.

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Authors and Affiliations

  1. Laboratory of Protein Engineering and Bio-active Molecules, National Institute of Applied Science and Technology - University of Carthage, Tunis, Tunisia

    Rahma Said, Nouha Setti Boubaker, Rim Jenni & Slah Ouerhani

  2. Laboratory of Genetics, Immunology and Human Pathology, Faculty of Sciences of Tunis, Tunis, Tunisia

    Karim Bougatef

  3. Urology Department, Charles Nicolle Hospital, Tunis, Tunisia

    Amine Derouiche & Mohamed Chebil

  4. Faculty of Sciences of Bizerte, University of Carthage, Carthage, Tunisia

    Rahma Said

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  1. Rahma Said
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Correspondence to Slah Ouerhani.

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The authors declare that they have no conflict of interest.

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This project was approved by a Charles Nicolle ethical committee, Tunis; Tunisia.

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Informed consent was obtained from all individual participants included in the study.

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Said, R., Bougatef, K., Setti Boubaker, N. et al. Polymorphisms in XPC gene and risk for prostate cancer. Mol Biol Rep 46, 1117–1125 (2019). https://doi.org/10.1007/s11033-018-4572-2

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  • DOI: https://doi.org/10.1007/s11033-018-4572-2

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