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A systematic proteomic analysis of NaCl-stressed germinating maize seeds

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Abstract

Salt (NaCl) is a common physiological stressor of plants. To better understand how germinating seeds respond to salt stress, we examined the changes that occurred in the proteome of maize seeds during NaCl-treated germination. Phenotypically, salt concentrations less than 0.2 M appear to delay germination, while higher concentrations disrupt development completely, leading to seed death. The identities of 96 proteins with expression levels altered by NaCl-incubation were established using 2-DE-MALDI-TOF–MS and 2-DE-MALDI-TOF–MS/MS. Of these 96 proteins, 79 were altered greater than twofold when incubated with a 0.2 M salt solution, while 51 were altered when incubated with a 0.1 M salt solution. According to their functional annotations in the Swiss-Prot protein-sequence databases, these proteins are mainly involved in seed storage, energy metabolism, stress response, and protein metabolism. Notably, the expression of proteins that respond to abscisic acid signals increased in response to salt stress. The results of this study provide important clues as to how NaCl stresses the physiology of germinating maize seeds.

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Abbreviations

ABA:

Abscisic acid

CHAPS:

3-(3-Chloamidopropyl)

DTT:

Dithiothreitol

IEF:

Isoelectric focusing

2-DE:

Two-dimensional polyacrylamide gel electrophoresis

MALDI-TOF–MS:

Matrix assisted laser desorption ionization-time of flight-mass spectrometry

MS/MS:

Tandem mass spectrometry

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Acknowledgments

This research was supported by grants from the National High-Tech Research and Development Program of China (Grant No. 2012AA10A300) and the Chinese Special Fund for Agro-scientific Research in the Public Interest (20093001-06-5).

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Authors and Affiliations

  1. Department of Life Science, Harbin University, Harbin, 150086, People’s Republic of China

    Ling-Bo Meng

  2. School of Horticulture, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Ling-Bo Meng

  3. Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, People’s Republic of China

    Yi-Bo Chen, Yue-Feng Wang & Bai-Chen Wang

  4. State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100093, People’s Republic of China

    Tian-Cong Lu

  5. Institute of Crop Cultivation and Farming, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, People’s Republic of China

    Chun-Rong Qian, Yang Yu & Xuan-Liang Ge

  6. Center of Agri-Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, People’s Republic of China

    Xiao-Hui Li

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  1. Ling-Bo Meng
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Correspondence to Xiao-Hui Li or Bai-Chen Wang.

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Ling-Bo Meng and Yi-Bo Chen have contributed equally to this work.

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Fig. S1 The MASCOT search results for HSP68 (spot 10) (DOC 296 kb)

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Meng, LB., Chen, YB., Lu, TC. et al. A systematic proteomic analysis of NaCl-stressed germinating maize seeds. Mol Biol Rep 41, 3431–3443 (2014). https://doi.org/10.1007/s11033-014-3205-7

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