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Proteomic profiling and redox status alteration of recalcitrant tea (Camellia sinensis) seed in response to desiccation

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Abstract

Tea seed is believed to be recalcitrant based on its sensitivity to chilling or drying stress. Reactive oxygen species (ROS) and alterations in cytosolic redox status have been implicated in intolerance to desiccation by recalcitrant seed, but there is little information available regarding how ROS are regulated in seeds susceptible to drying stress. We investigated changes in protein expression and activity in tea embryo in response to desiccation using physiological and proteomic methods. Results showed that desiccation treatment dramatically induced the accumulation of H2O2 in tea embryos, accompanied by increased activities of antioxidant enzymes like ascorbate peroxidase (APX) and superoxide dismutase (SOD). Proteomic analyses also demonstrated that 23 proteins associated with defense response, metabolism and redox status were up-regulated following desiccation. Increase in antioxidants, ascorbic acid (AsA) and catalase (CAT) (H2O2 scavengers) partially assuaged desiccation damage to tea seed, resulting in improved germination rates. Higher accumulation of H2O2 aggravated desiccation damage to seeds leading to lower germination activity. We propose that desiccation causes an over-accumulation of ROS that are not efficiently scavenged by increased levels of antioxidant enzymes. High levels of ROS alter the redox status and are detrimental to seed viability. Reducing ROS to appropriate concentrations is an efficient way to reduce desiccation damage and improve germination rates of recalcitrant seeds.

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Abbreviations

AsA:

Ascorbic acid

APX:

Ascorbate peroxidase

BHT:

Butylated hydroxytoluene

CAT:

Catalase

DHAR:

Dehydroascorbate reductase

GSH:

Glutathione

GR:

Glutathione reductase

MDAR:

Monodehydroascorbate reductase

POD:

Peroxidase

DMTU:

Dimethylthiourea

ROS:

Reactive oxygen species

SHAM:

Salicylhydroxamic acid

SOD:

Superoxide dismutase

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Acknowledgments

This work was supported by 100 Talents Program of CAS (to X.H.) and National Science Foundation grant of China (No. 30871704 and No. 30971452 to X.H., No. 30900871 to L.Y.). We deeply thank two anonymous reviewers for helpful suggestions and correcting English expression.

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Authors and Affiliations

  1. Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Institute of Tibet Plateau Research at Kunming, Chinese Academy of Sciences, Kunming, 650204, China

    Qi Chen, Liming Yang & Xiangyang Hu

  2. Key Laboratory of Tea Biochemistry and Biotechnology, Anhui Agricultural University, West 130 Changjiang Road, Hefei, Anhui, 230036, China

    Qi Chen & Xiaochun Wan

  3. School of life Science, Huaiyin Normal University, Huaiyin, 223300, China

    Liming Yang

  4. Department of Botany, A.S. College, University of Kashmir, Srinagar, 190008, India

    Parvaiz Ahmad

Authors
  1. Qi Chen
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  2. Liming Yang
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  3. Parvaiz Ahmad
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  4. Xiaochun Wan
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  5. Xiangyang Hu
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Corresponding author

Correspondence to Xiangyang Hu.

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Q. Chen and L. Yang contributed equally to this work.

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Chen, Q., Yang, L., Ahmad, P. et al. Proteomic profiling and redox status alteration of recalcitrant tea (Camellia sinensis) seed in response to desiccation. Planta 233, 583–592 (2011). https://doi.org/10.1007/s00425-010-1322-7

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