Abstract
GAST (GA-stimulated transcript)-like genes have been reported as targets of GA regulation in some plant species. In this study, we isolated seven GAST-like cDNAs from cotton (Gossypium hirsutum) cDNA libraries (designated as GhGASL1–GhGASL7). Meanwhile, the genomic DNA clones corresponding to the seven GhGASL genes were isolated by using PCR amplification technique. Analysis of gene structure revealed that four genes (GhGASL1/3/5/6) contain two exons and one intron, while the rest have four exons and three introns. All of the deduced GhGASL proteins contain a putative signal peptide in the N-terminus and a conservative cysteine-rich C-terminal domain. Quantitative RT-PCR analysis indicated that the seven GhGASL genes are differentially expressed in cotton tissues. Among them, GhGASL1/4/7 were predominantly expressed in cotyledons, while the transcripts of GhGASL2/5 were preferentially accumulated at hypocotyls. GhGASL3 mRNA was largely accumulated in fibers, while GhGASL6 transcripts were mainly detected in ovules. Furthermore, GhGASL2/3/5 displayed a relatively high expression levels during early fiber elongation stages, and were regulated by GA. These data suggested that GhGASL genes may be involved in fiber elongation and in response to GA signaling during fiber development.
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References
Sun TP, Gubler F (2004) Molecular mechanism of gibberellin signaling in plants. Annu Rev Plant Biol 55:197–223
Li HP, Wang Y, Li XC, Gao Y, Wang ZJ, Zhao Y, Wang ML (2011) A GA-insensitive dwarf mutant of Brassica napus L. Correlated with mutation in pyrimidine box in the promoter of GID1. Mol Biol Rep 38:191–197
Gao SJ, Xie XZ, Yang SG, Chen ZP, Wang XJ (2012) The changes of GA level and signaling are involved in the regulation of mesocotyl elongation during blue light mediated de-etiolation in Sorghum bicolor. Mol Biol Rep 39:4091–4100
Guo RY, Yu FF, Gao Z, An HL, Cao XC, Guo XQ (2011) GhWRKY3, a novel cotton (Gossypium hirsutum L.) WRKY gene, is involved in diverse stress responses. Mol Biol Rep 38:49–58
Gao Y, Chen JM, Zhao Y, Li TT, Wang ML (2012) Molecular cloning and expression analysis of a RGA-like gene responsive to plant hormones in Brassica napus. Mol Biol Rep 39:1957–1962
Gao XH, Xiao SL, Yao QF, Wang YJ, Fu XD (2011) An updated GA signaling ‘relief of repression’ regulatory model. Mol Plant 4:601–606
Shi L, Gast RT, Gopalraj M, Olszewski NE (1992) Characterization of a shoot-specific, GA3- and ABA-regulated gene from tomato. Plant J 2:153–159
Taylor BH, Scheuring CF (1994) A molecular marker for lateral root initiation: the RSI-1 gene of tomato (Lycopersicon esculentum Mill) is activated in early lateral root primordial. Mol Gen Genet 243:148–157
Ben-Nissan G, Weiss D (1996) The petunia homologue of tomato gast1: transcript accumulation coincides with gibberellin-induced corolla cell elongation. Plant Mol Biol 32:1067–1074
Segura A, Moreno M, Madueño F, Molina A, García-Olmedo F (1999) Snakin-1, a peptide from potato that is active against plant pathogens. Mol. Plant-Microbe Interact 12:16–23
Berrocal-Lobo M, Segura A, Moreno M, López G, García-Olmedo F, Molina A (2002) Snakin-2, an antimicrobial peptide from potato whose gene is locally induced by wounding and responds to pathogen infection. Plant Physiol 128:951–961
Herzog M, Dorne AM, Grellet F (1995) GASA, a gibberellin-regulated gene family from Arabidopsis thaliana related to the tomato GAST1 gene. Plant Mol Biol 27:743–752
Aubert D, Chevillard M, Dorne AM, Arlaud G, Herzog M (1998) Expression patterns of GASA genes in Arabidopsis thaliana: the GASA4 gene is upregulated by gibberellins in meristematic regions. Plant Mol Biol 36:871–883
Kotilainen M, Helariutta Y, Mehto M, Pollanen E, Albert VA, Elomaa P, Teeri TH (1999) GEG participates in the regulation of cell and organ shape during corolla and carpel development in Gerbera hybrida. Plant Cell 11:1093–1104
Zimmermann R, Sakai H, Hochholdinger F (2010) The gibberellic acid stimulated-like gene family in maize and its role in lateral root development. Plant Physiol 152:356–365
Li KL, Bai X, Li Y, Cai H, Ji W, Tang LL, Wen YD, Zhu YM (2011) GsGASA1 mediated root growth inhibition in response to chronic cold stress is marked by the accumulation of DELLAs. J Plant Physiol 168:2153–2160
Zhang SC, Wang XJ (2011) Overexpression of GASA5 increases the sensitivity of Arabidopsis to heat stress. J Plant Physiol 168:2093–2101
Gialvalis S, Seagull RW (2001) Plant hormones alter fiber initiation in unfertilized, cultured ovules of Gossypium hirsutum. J Cotton Sci 5:252–258
Shi YH, Zhu SW, Mao XZ, Feng JX, Qin YM, Zhang L (2006) Transcriptome profiling, molecular biological, and physiological studies reveal a major role for ethylene in cotton fiber cell elongation. Plant Cell 18:651–664
Yang SS, Cheung F, Lee JJ, Ha M, Wei NE, Sze SH (2006) Accumulation of genome-specific transcripts, transcription factors and phytohormonal regulators during early stages of fiber cell development in allotetraploid cotton. Plant J 47:761–775
Aleman L, Kitamura J, Abdel-Mageed H, Lee J, Sun Y, Nakajima M (2008) Functional analysis of cotton orthologs of GA signal transduction factors GID1 and SLR1. Plant Mol Biol 68:1–16
Dong J, Yin MH, Yang F, Zhao J, Qin S, Hou L (2009) Cloning and expression profiling of gibberellin insensitive dwarf GID1 homologous genes from cotton. Acta Agron Sin 35:1822–1830
Beasley CA, Ting IP (1974) Effects of plant growth substances on in vitro fiber development from unfertilized cotton ovules. Am J Bot 61:188–194
Li XB, Cai L, Cheng NH, Liu JW (2002) Molecular characterization of the cotton GhTUB1 gene that is preferentially expressed in fiber. Plant Physiol 130:666–674
Li DD, Wu YJ, Ruan XM, Li B, Zhu L, Wang H, Li XB (2009) Expressions of three cotton genes encoding the PIP proteins are regulated in root development and in response to stresses. Plant Cell Rep 28:291–300
Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinf 5:150–163
Rubinovich L, Weiss D (2010) The Arabidopsis cysteine-rich protein GASA4 promotes GA responses and exhibits redox activity in bacteria and in planta. Plant J 64:1018–1027
Ben-Nissan G, Lee JY, Borohov A, Weiss D (2004) GIP, a Petunia hybrida GA-induced cysteine-rich protein: a possible role in shoot elongation and transition to flowering. Plant J 37:229–238
Beasley CA, Ting IP (1973) The effects of plant growth substances on in vitro fiber development from fertilized cotton ovules. Am J Bot 60:130–139
Chen JG, Du XM, Zhao HY, Zhou X (1996) Fluctuation in levels of endogenous plant hormones in ovules of normal and mutant cotton during flowering and their relation to fiber development. J Plant Growth Regul 15:173–177
Momtaz OA (1998) Effect of plant growth regulators on in vitro fiber development from unfertilized and fertilized Egyptian cotton ovules. Plant Growth Regul 25:159–164
Gokani SJ, Thaker VS (2002) Role of gibberellic acid in cotton fibre development. J Agric Sci 138:255–260
Aleman L, Kitamura J, Abdel-mageed H, Lee J, Sun Y, Nakajima M, Ueguchi-Tanaka M, Matsuoka M, Allen RD (2008) Functional analysis of cotton orthologs of GA signal transduction factors GID1 and SLR1. Plant Mol Biol 68:1–16
Xiao YH, Li DM, Yin MH, Li XB, Zhang M, Wang YJ, Dong J, Zhao J, Luo M, Luo XY, Hou L, Hu L, Pei Y (2010) Gibberellin 20-oxidase promotes initiation and elongation of cotton fibers by regulating gibberellin synthesis. J Plant Physiol 167:829–837
Zentella R, Zhang ZL, Park M, Thomas SG, Endo A, Murase K, Fleet CM, Jikumaru Y, Nambara E, Kamiya Y, Sun TP (2007) Global analysis of DELLA direct targets in early gibberellin signaling in Arabidopsis. Plant Cell 19:3037–3057
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This work was supported by National Natural Sciences Foundation of China (grant No. 31171174), the project of Hubei Key Laboratory of Genetic Regulation and Integrative Biology (Grant No. 201008) and the Scientific Research Foundation of Hubei Province (Grant No. 2011CDA140).
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Liu, ZH., Zhu, L., Shi, HY. et al. Cotton GASL genes encoding putative gibberellin-regulated proteins are involved in response to GA signaling in fiber development. Mol Biol Rep 40, 4561–4570 (2013). https://doi.org/10.1007/s11033-013-2543-1
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DOI: https://doi.org/10.1007/s11033-013-2543-1