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GhWRKY3, a novel cotton (Gossypium hirsutum L.) WRKY gene, is involved in diverse stress responses

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Abstract

WRKY proteins play important roles in plant defense responses. In the present study, a novel WRKY gene, nominated as GhWRKY3, was isolated from cotton (Gossypium hirsutum L.). The full-length cDNA of GhWRKY3 is 1,705 bp in length and encodes a protein with 507 amino acids containing two typical WRKY domains and two zinc finger motifs. Amino acid sequence alignment revealed that GhWRKY3 shares a high degree of identity with other higher plant WRKY proteins. The subcellular localization assay indicated that GhWRKY3 is localized to the nucleus. Analysis of 5′-flanking region of GhWRKY3 revealed a group of putative cis-acting elements. The results of expression analysis indicated that GhWRKY3 is constitutively expressed in roots, stems and leaves. Semi-quantitative RT-PCR showed that GhWRKY3 is up-regulated by application of various phytohormones including salicylic acid (SA), methyl jasmonate (MeJA), abscisic acid (ABA), gibberellins (GAs) and ethylene (ET). Furthermore, the transcripts of GhWRKY3 are enhanced after infection with Rhizoctonia solani, Colletotrichum gossypii and Fusarium oxysporum f. sp. vasinfectum, respectively. Also, GhWRKY3 can be induced by wounding treatment, but not by cytokinin (6-benzylaminopurine, 6-BA), auxin analogue, drought, NaCl, and cold (4°C). These data suggested that GhWRKY3 might play an important role in plant defense responses and fulfill a pivotal role in plant development.

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Abbreviations

ABA:

Abscisic acid

6-BA:

6-Benzylaminopurine

C. gossypii:

Colletotrichum gossypii

E. coli :

Escherichia coli

ET:

Ethylene

F. oxysporum f. sp. vasinfectum:

Fusarium oxysporum f. sp. vasinfectum

GAs:

Gibberellins

H2O2 :

Hydrogen peroxide

I-PCR:

Inverse polymerase chain reaction

MeJA:

Methyl jasmonate

NAA:

α-Naphthlcetic acid

NLS:

Nuclear localization signal

ORF:

Open reading frame

RACE:

Rapid amplification of cDNA ends

RT-PCR:

Reverse transcript PCR

R. solani:

Rhizoctonia solani

SA:

Salicylic acid

SAR:

Systemic acquired resistance

References

  1. Rajendra B, Jones JD (2009) Role of plant hormones in plant defence responses. Plant Mol Biol 69:473–488. doi:10.1007/s11103-008-9435-0

    Article  Google Scholar 

  2. Chen WJ, Zhu T (2004) Networks of transcription factors with roles in environmental stress response. Trends Plant Sci 9:591–596. doi:10.1016/j.tplants.2004.10.007

    Article  CAS  PubMed  Google Scholar 

  3. Riechmann JL, Heard J, Martin G, Reuber L, Jiang C, Keddie J, Adam L, Pineda O, Ratcliffe OJ, Samaha RR, Creelman R, Pilgrim M, Broun P, Zhang JZ, Ghandehari D, Sherman BK, Yu G (2000) Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science 290:2105–2110. doi:10.1126/science.290.5499.2105

    Article  CAS  PubMed  Google Scholar 

  4. Eulgem T, Rushton PJ, Robatzek S, Somssich IE (2000) The WRKY superfamily of plant transcription factors. Trends Plant Sci 5:199–206. doi:10.1016/S1360-1385(00)01600-9

    Article  CAS  PubMed  Google Scholar 

  5. Ulker B, Somssich IE (2004) WRKY transcription factors: from DNA binding towards biological function. Curr Opin Plant Biol 7:491–498. doi:10.1016/j.pbi.2004.07.012

    Article  PubMed  Google Scholar 

  6. Eulgem T, Somssich IE (2007) Networks of WRKY transcription factors in defense signaling. Curr Opin Plant Biol 10:366–371. doi:10.1016/j.pbi.2007.04.020

    Article  CAS  PubMed  Google Scholar 

  7. Ross CA, Liu Y, Shen QJ (2007) The WRKY gene family in rice (Oryza sativa). J Integr Plant Biol 49:827–842. doi:10.1111/j.1672-9072.2007.00504.x

    Article  CAS  Google Scholar 

  8. Pan YJ, Cho CC, Kao YY, Sun CH (2009) A novel WRKY-like protein involved in transcriptional activation of cyst wall protein genes in Giardia lamblia. J Biol Chem 284:17975–17988. doi:10.1074/jbc.M109.012047

    Article  CAS  PubMed  Google Scholar 

  9. Zhang Y, Wang L (2005) The WRKY transcription factor superfamily: its origin in eukaryotes and expansion in plants. BMC Evol Biol 5:1. doi:10.1186/1471-2148-5-1

    Article  CAS  PubMed  Google Scholar 

  10. Dong J, Chen C, Chen Z (2003) Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response. Plant Mol Biol 51:21–37. doi:10.1023/A:1020780022549

    Article  CAS  PubMed  Google Scholar 

  11. Yang B, Jiang Y, Rahman MH, Deyholos MK, Kav NN (2009) Identification and expression analysis of WRKY transcription factor genes in canola (Brassica napus L.) in response to fungal pathogens and hormone treatments. BMC Plant Biol 9:68. doi:10.1186/1471-2229-9-68

    Article  PubMed  Google Scholar 

  12. Li J, Brader G, Palva ET (2004) The WRKY70 transcription factor: a node of convergence for jasmonate-mediated and salicylate-mediated signals in plant defense. Plant Cell 16:319–331. doi:10.1105/tpc.016980

    Article  CAS  PubMed  Google Scholar 

  13. Li J, Brader G, Kariola T, Tapio Palva E (2006) WRKY70 modulates the selection of signaling pathways in plant defense. Plant J 46:477–491. doi:10.1111/j.1365-313X.2006.02712.x

    Article  CAS  PubMed  Google Scholar 

  14. Ulker B, Shahid Mukhtar M, Somssich IE (2007) The WRKY70 transcription factor of Arabidopsis influences both the plant senescence and defense signaling pathways. Planta 226:125–137. doi:10.1007/s00425-006-0474-y

    Article  PubMed  Google Scholar 

  15. Mao P, Duan M, Wei C, Li Y (2007) WRKY62 transcription factor acts downstream of cytosolic NPR1 and negatively regulates jasmonate responsive gene expression. Plant Cell Physiol 48:833–842. doi:10.1093/pcp/pcm058

    Article  CAS  PubMed  Google Scholar 

  16. Mare C, Mazzucotelli E, Crosatti C, Francia E, Stanca MA, Cattivelli L (2004) Hv-WRKY38: a new transcription factor involved in cold- and drought-response in barley. Plant Mol Biol 55:399–416. doi:10.1007/s11103-004-0906-7

    Article  CAS  PubMed  Google Scholar 

  17. Hara K, Yagi M, Kusano T, Sano H (2000) Rapid systemic accumulation of transcripts encoding a tobacco WRKY transcription factor upon wounding. Mol Gen Genet 263:30–37. doi:10.1007/PL00008673

    Article  CAS  PubMed  Google Scholar 

  18. Xu YH, Wang JW, Wang S, Wang JY, Chen XY (2004) Characterization of GaWRKY1, a cotton transcription factor that regulates the sesquiterpene synthase gene (+)-δ-cadinene synthase-A. Plant Physiol 135:507–515. doi:10.1104/pp.104.038612

    Article  CAS  PubMed  Google Scholar 

  19. Taliercio E, Ray J, Scheffler J (2010) Isolation and characterization of a cotton cdh-like gene. Mol Biol Rep 37:643–648. doi:10.1007/s11033-009-9496-4

    Article  CAS  PubMed  Google Scholar 

  20. Chen D, Ding Y, Guo W, Zhang T (2009) Molecular cloning and characterization of a flower-specific class III peroxidase gene in G. Hirsutum. Mol Biol Rep 36:461–469. doi:10.1007/s11033-007-9202-3

    Article  CAS  PubMed  Google Scholar 

  21. Li F, Zhang Y, Wang M, Zhang Y, Wu X, Guo X (2008) Molecular cloning and expression characteristics of alternative oxidase gene of cotton (Gossypium hirsutum). Mol Biol Rep 35:97–105. doi:10.1007/s11033-007-9058-6

    Article  CAS  PubMed  Google Scholar 

  22. Gao Q, Liu Y, Wang M, Zhang J, Gai Y, Zhu C, Guo X (2009) Molecular cloning and characterization of an inducible RNA-dependent RNA polymerase gene, GhRdRP, from cotton (Gossypium hirsutum L.). Mol Biol Rep 36:47–56. doi:10.1007/s11033-007-9150-y

    Article  PubMed  Google Scholar 

  23. Yang L, Zhu H, Guo W, Zhang T (2010) Molecular cloning and characterization of five genes encoding pentatricopeptide repeat proteins from Upland cotton (Gossypium hirsutum L.). Mol Biol Rep 37:801–808. doi:10.1007/s11033-009-9610-7

    Article  CAS  PubMed  Google Scholar 

  24. Porebski S, Bailey LG, Baum BR (1997) Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Rep 15:8–15. doi:10.1007/BF02772108

    Article  CAS  Google Scholar 

  25. Varagona MJ, Schmidt RJ, Raikhel NV (1992) Nuclear localization signal(s) required for nuclear targeting of the maize regulatory protein Opaque-2. Plant Cell 4:1213–1227. doi:10.1105/tpc.4.10.1213

    Article  CAS  PubMed  Google Scholar 

  26. Wu KL, Guo ZJ, Wang HH, Li J (2005) The WRKY family of transcription factor in rice and Arabidopsis and their origins. DNA Res 12:9–26. doi:10.1093/dnares/12.1.9

    Article  CAS  PubMed  Google Scholar 

  27. Robert-Seilaniantz A, Navarro L, Bari R, Jone DG (2007) Pathological hormone imbalances. Curr Opin Plant Biol 10:372–379. doi:10.1016/j.pbi.2007.06.003

    Article  CAS  PubMed  Google Scholar 

  28. Laloi C, Apel K, Danon A (2004) Reactive oxygen signalling: the latest news. Curr Opin Plant Biol 7:323–328. doi:10.1016/j.pbi.2004.03.005

    Article  CAS  PubMed  Google Scholar 

  29. Overmyer K, Brosche M, Kangasjarvi J (2003) Reactive oxygen species and hormonal control of cell death. Trends Plant Sci 8:335–342. doi:10.1016/S1360-1385(03)00135-3

    Article  CAS  PubMed  Google Scholar 

  30. Singh KB, Fole RC, Onate-Sanchez L (2002) Transcription factors in plant defense and stress responses. Curr Opin Plant Biol 5:430–436. doi:10.1016/S1369-5266(02)00289-3

    Article  CAS  PubMed  Google Scholar 

  31. Pandey SP, Somssich IE (2009) The role of WRKY transcription factor in plant immunity. Plant Physiol 150:1648–1655. doi:10.1104/pp.109.138990

    Article  CAS  PubMed  Google Scholar 

  32. Maeo K, Hayashi S, Kojima-Suzuki H, Morikami A, Nakamura D (2001) Role of conserved residues of the WRKY domain in the DNA-binding of tobacco WRKY family proteins. Biosci Biotechnol Biochem 65:2428–2436. doi:10.1271/bbb.65.2428

    Article  CAS  PubMed  Google Scholar 

  33. Dong XN (1996) SA, JA, ethylene, and disease resistance in plants. Curr Opin Plant Biol 1:316–323. doi:10.1016/1369-5266(88)80053-0

    Article  Google Scholar 

  34. Kessler A, Baldwin IT (2002) Plant responses to insect herbivory: the emerging molecular analysis. Annu Rev Plant Biol 53:299–328. doi:10.1146/annurev.arplant.53.100301.135207

    Article  CAS  PubMed  Google Scholar 

  35. Ryals JA, Neuenschwander UH, Willits MG, Molina A, Steiner HY, Hunt MD (1996) Systemic acquired resistance. Plant Cell 8:1809–1819. doi:10.1105/tpc.8.10.1809

    Article  CAS  PubMed  Google Scholar 

  36. Koornneef A, Pieterse CM (2008) Cross talk in defense signaling. Plant Physiol 146:839–844. doi:10.1104/pp.107.112029

    Article  CAS  PubMed  Google Scholar 

  37. Lai ZB, Vinod KM, Zheng ZY, Fan BF, Chen ZX (2008) Roles of Arabidopsis WRKY3 and WRKY4 transcription factors in plant responses to pathogens. BMC Plant Biol 8:68. doi:10.1186/1471-2229-8-68

    Article  PubMed  Google Scholar 

  38. Chinnusamy V, Gong ZZ, Zhu JK (2008) Abscisic acid-mediated epigenetic processes in plant development and stress responses. J Integr Plant Biol 50:1187–1195. doi:10.1111/j.1744-7909.2008.00727.x

    Article  CAS  PubMed  Google Scholar 

  39. Olszewski N, Sun TP, Gubler F (2002) Gibberellin signaling: biosynthesis, catabolism, and response pathways. Plant Cell 14:S61–S80. doi:10.1105/tpc.010476

    CAS  PubMed  Google Scholar 

  40. Zhang ZL, Shin M, Zou X, Huang J, David Ho TH, Shen QJ (2009) A negative regulator encoded by a rice WRKY gene represses both abscisic acid and gibberellins signaling in aleurone cells. Plant Mol Biol 70:139–151. doi:10.1007/s11103-009-9463-4

    Article  CAS  PubMed  Google Scholar 

  41. Robatzek S, Somssich IE (2001) A new member of the Arabidopsis WRKY transcription factor family, AtWRKY6, is associated with both senescence- and defense-related processes. Plant J 28:123–133. doi:10.1046/j.1365-313X.2001.01131.x

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (No. 30571215) and China National Transgenic Plant Research and Commercialization Project (No. 2009ZX08009-113B).

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, People’s Republic of China

    Ruoyu Guo, Feifei Yu, Zheng Gao, Hailong An & Xingqi Guo

  2. College of Information Science and Engineering, Shandong Agricultural University, Taian, Shandong, 271018, People’s Republic of China

    Xuecheng Cao

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  1. Ruoyu Guo
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Correspondence to Xuecheng Cao or Xingqi Guo.

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Guo, R., Yu, F., Gao, Z. et al. GhWRKY3, a novel cotton (Gossypium hirsutum L.) WRKY gene, is involved in diverse stress responses. Mol Biol Rep 38, 49–58 (2011). https://doi.org/10.1007/s11033-010-0076-4

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