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Genetic data provided by 21 autosomal STR loci from Chinese Tujia ethnic group

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Abstract

The aim of this study was to investigate allelic frequency distribution and forensic genetic parameters of autosomal short tandem repeats (STR) loci of the population samples from 107 Tujia individuals from Chinese Hubei Province. Twenty-one autosomal STR genetic markers (D9S1122, D6S474, D6S1017, D5S2500, D4S2408, D3S4529, D2S441, D2S1776, D22S1045, D20S482, D1S1677, D1S1627, D1GATA113, D19S433, D18S853, D17S1301, D11S4463, D12ATA63, D10S1248, D10S1435 and D14S1434) were simultaneously amplified in a new multiplex polymerase chain reaction system. 155 alleles for all the STR loci from the Tujia population were observed and the corresponding allelic frequencies ranged from 0.005 to 0.589. Expected heterozygosity, polymorphic information content, power of discrimination and power of exclusion of the 21 STR loci in the Tujia population were from 0.579 to 0.824, from 0.525 to 0.802, from 0.773 to 0.945 and from 0.257 to 0.641, respectively. Our results indicate that the autosomal STRs multiplex system provides highly informative STR data and could be useful in forensic individual identification and parentage testing in this region.

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References

  1. Edwards A, Civitello A, Hammond HA, Caskey CT (1991) DNA typing and genetic mapping with trimeric and tetrameric tandem repeats. Am J Hum Genet 49:746–756

    PubMed  CAS  Google Scholar 

  2. Edwards A, Hammond HA, Jin L, Caskey CT, Chakraborty R (1992) Genetic variation at five trimeric and tetrameric tandem repeat loci in four human population groups. Genomics 12:241–253

    Article  PubMed  CAS  Google Scholar 

  3. Hammond HA, Jin LI, Zhong Y, Zhong Y, Caskey CT, Chakaborty R (1994) Evaluation of 13 short tandem repeat loci for use in personal identification applications. Am J Hum Genet 55:175–189

    PubMed  CAS  Google Scholar 

  4. Zhu BF, Lü GP, Shen CM, Yao GF, Tian YF, Li T, Wang ZY (2002) Genetic Polymorphism of 9 STR Loci in Han Nation Population in Shandong Province. Acta Acad Med Sin 24:620–625 (in Chinese)

    Google Scholar 

  5. Lin H, Fan H, Zhang F, Huang X, Lin K, Shi L, Hu S, Chu J, Wang DM (2010) Genetic relationships of ethnic minorities in Southwest China revealed by microsatellite markers. PLoS ONE 5:e9895

    Article  PubMed  Google Scholar 

  6. Deng YJ, Zhu BF, Shen CM, Wang HD, Huang JF, Li YZ, Qin HX, Mu HF, Su J, Wu J, Zhang B, Fan SL (2011) Genetic polymorphism analysis of 15 STR loci in Chinese Hui ethnic group residing in Qinghai Province of China. Mol Biol Rep 38(4):2315–2322

    Article  PubMed  CAS  Google Scholar 

  7. Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506–513

    PubMed  CAS  Google Scholar 

  8. Bär W, Brinkmann B, Budowle B, Carracedo A, Gill P, Lincoln P, Mayr W, Olaisen B (1997) DNA recommendations. Further report of the DNA commission of the ISFH regarding the use of short tandem repeat systems. Int J Leg Med 110:175–176

    Article  Google Scholar 

  9. Zhao F, Wu XY, Cai GQ, Xu CC (2003) The Applications of Modified-Powerstates software in the forensic biostatistics. J Forensic Med 18:297–299 (in Chinese)

    Google Scholar 

  10. Tereba A (1999) Tools for analysis of population statistics. profiles in DNA, vol 2. Promega Corporation, Madison, pp 14–16

    Google Scholar 

  11. Excoffier L, Laval G, Schneider S (2007) Arlequin ver.3.0: An integrated software package for Population genetics data analysis. Evol Bioinform Online 1:47–50

    PubMed  Google Scholar 

  12. Brown MJ (2001) Ethnic classification and culture: the case of the Tujia in Hubei, China. Asian Ethnicity 2(1):55–72

    Article  Google Scholar 

  13. Gusmão L, Sánchez-Diz P, Alves C, Gomes I, Zarrabeitia MT, Abovich M et al (2009) A GEP-ISFG collaborative study on the optimization of an X-STR decaplex: data on 15 Iberian and Latin American populations. Int J Legal Med 123(3):227–234

    Article  PubMed  Google Scholar 

  14. Hering S, Brundirs N, Kuhlisch E, Edelmann J, Plate I, Benecke M et al (2004) DXS10011: studies on structure, allele distribution in three populations and genetic linkage to further q-telomeric chromosome X markers. Int J Legal Med 118(6):313–319

    Article  PubMed  Google Scholar 

  15. Zhu BF, Shen CM, Wang HD, Yang G, Yan JW, Qin HX, Guo JX, Huang JF, Jing H, Liu XS (2011) Genetic diversities of 21 non-CODIS autosomal STRs of a Chinese Tibetan ethnic minority group in Lhasa. Int J Legal Med 125(4):581–585

    Article  PubMed  Google Scholar 

  16. Teng Y, Zhang FX, Shen CM, Wang F, Wang HD, Yan JW, Liu JL (2012) Genetic variation of new 21 autosomal short tandem repeat loci in a Chinese Salar ethnic group. Mol Biol Rep 39(2):1465–1470

    Article  PubMed  CAS  Google Scholar 

  17. Yuan L, Ge J, Lu D, Yang X (2012) Population data of 21 non-CODIS STR loci in Han population of northern China. Int J Legal Med 126(4):659–664

    Article  PubMed  Google Scholar 

  18. Deng Y, Zhu B, Yu X, Li Y, Fang J, Xiong X, Mu H, Huang Y, Shi X (2007) Genetic polymorphisms of 15 STR loci of Chinese Dongxiang and Salar ethnic minority living in Qinghai Province of China. Leg Med (Tokyo) 9:38–42

    Article  CAS  Google Scholar 

  19. Zhu B, Yan J, Shen C, Li T, Li Y, Yu X, Xiong X, Mu H, Huang Y, Deng Y (2008) Population genetic analysis of 15 STR loci of Chinese Tu ethnic minority group. Forensic Sci Int 174:255–258

    Article  PubMed  CAS  Google Scholar 

  20. Chen L, He Y, Li S (2006) Genetic analysis of 15 STR loci of Chinese Ewenki ethnic population. J Forensic Sci 51:1408–1409

    Article  PubMed  CAS  Google Scholar 

  21. Zhu BF, Shen CM, Wu QJ, Deng YJ (2008) Population data of 15 STR loci of Chinese Yi ethnic minority group. Leg Med (Tokyo) 10:220–224

    Article  CAS  Google Scholar 

  22. Zhu BF, Yang G, Shen CM, Qin HX, Fan SL, Deng LB, Chen F, Chen LP, Wang HD, Wang ZY, Lucas R, Lian ZM (2010) Population genetic analysis of 15 autosomal STR loci in the Russian population of northeastern Inner-Mongolia, China. Mol Biol Rep 37:3889–3895

    Article  PubMed  CAS  Google Scholar 

  23. Wu YM, Zhang XN, Zhou Y, Chen ZY, Wang XB (2008) Genetic polymorphisms of 15 STR loci in Chinese Han population living in Xi’an city of Shaanxi Province. Forensic Sci Int Genet 2:e15–e18

    Article  PubMed  Google Scholar 

  24. Zhu J, Li J, Guo Y, Liu K, Zhu B, Liu Y (2005) Population data of 15 STR in Chinese Han population from north of Guangdong. J Forensic Sci 50:1510–1511

    PubMed  CAS  Google Scholar 

  25. Zhu B, Wang Z, Wu Q, Sedike ME, Zhu J, Huang P, Xu Y, Liu Y (2005) Genetic analysis of 15 STR loci of Chinese Uigur ethnic population. J Forensic Sci 50:1235–1236

    PubMed  CAS  Google Scholar 

  26. Yong RY, Gan LS, Chua EM, Yap EP (2009) Polymorphism studies of six miniSTR loci for three ethnic populations in Singapore. Leg Med (Tokyo) 11(4):195–197

    Article  CAS  Google Scholar 

  27. Abrahams Z, D’Amato ME, Davison S, Benjeddou M (2011) Allele frequencies of six non-CODIS miniSTR loci (D1S1627, D3S4529, D5S2500, D6S1017, D8S1115 and D9S2157) in three South African populations. Forensic Sci Int Genet 5(4):354–355

    Article  PubMed  CAS  Google Scholar 

  28. Raimann PE, de Oliveira AP, Rodenbusch R, Picanço JB, Albuquerque TC, Alho CS (2012) Genetic data for D1S1677, D2S441, D4S2364, D10S1248, D14S1434 and D22S1045 miniSTR loci from the state of Rio Grande do Sul, Southern Brazil. Forensic Sci Int Genet 6(1):42–43

    Article  Google Scholar 

  29. Cortellini V, Cerri N, Verzeletti A (2011) Genetic variation at 5 new autosomal short tandem repeat markers (D10S1248, D22S1045, D2S441, D1S1656, D12S391) in a population-based sample from Maghreb region. Croat Med J 52(3):368–371

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This project was supported by the National Natural Science Foundation of China (NSFC, No. 31271348), the Program for New Century Excellent Talents of the Ministry of Education, China (NECT-10-0687); supported by the Fundamental Research Funds for the Central University, China (2011jdgz20); and the Shaan’xi Provincial Science and Technology Research and Development Project Fund (No. 2012K16-08-01), China.

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Authors and Affiliations

  1. Scientific Research and Experiment Center, The Second Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an, 710004, People’s Republic of China

    Guo-lian Yuan

  2. The Key Laboratory of Environment and Gene Related to Diseases (Xi’an Jiaotong University), Ministry of Education, Xi’an, 710061, People’s Republic of China

    Guo-lian Yuan & Bofeng Zhu

  3. Blood Center of the Shaanxi Province, Shaanxi, 710061, People’s Republic of China

    Chun-mei Shen

  4. Medical Genetic Institute of Henan Province, People’s Hospital of Henan Province, Zhengzhou, 450003, People’s Republic of China

    Hong-dan Wang

  5. School of Medicine, Xi’an Jiaotong University, Xi’an, 710061, People’s Republic of China

    Hong-dan Wang & Wen-juan Liu

  6. Vascular Biology Center, Georgia Health Sciences University, Augusta, GA, 30912, USA

    Guang Yang

  7. Beijing Institute of Genomics, Chinese Academy of Science, Beijing, 100029, People’s Republic of China

    Jiang-wei Yan

  8. Department of Internal Medicine, Morehouse School of Medicine, Atlanta, GA, 30303, USA

    Hai-Xia Qin

  9. School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510275, People’s Republic of China

    Tong Xie

  10. Affiliated Hospital of Hubei Institute for Nationalities, Enshi, 445000, People’s Republic of China

    Hangyan Ran, Jie Yuan & Zuochun Liu

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  1. Guo-lian Yuan
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  2. Chun-mei Shen
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  5. Guang Yang
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  8. Tong Xie
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  9. Hangyan Ran
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  10. Jie Yuan
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  11. Zuochun Liu
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  12. Bofeng Zhu
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Correspondence to Guo-lian Yuan.

Additional information

Guo-lian Yuan and Bo-feng Zhu have contributed equally to this work and are co-corresponding authors.

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Yuan, Gl., Shen, Cm., Wang, Hd. et al. Genetic data provided by 21 autosomal STR loci from Chinese Tujia ethnic group. Mol Biol Rep 39, 10265–10271 (2012). https://doi.org/10.1007/s11033-012-1903-6

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  • DOI: https://doi.org/10.1007/s11033-012-1903-6

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