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Genetic diversities of 21 non-CODIS autosomal STRs of a Chinese Tibetan ethnic minority group in Lhasa

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Abstract

In the present study, we investigated 21 short tandem repeat (STR) loci (D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D19S433, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435, D5S2500), which are not included in the Combined DNA Index System and Amelogenin locus in 104 randomly selected healthy autochthonous individuals from the Tibetan ethnic minority group residing in the Lhasa region, Tibet Autonomous Region of China. Allelic frequencies, common forensic statistical parameters, and the Hardy–Weinberg equilibrium in this population were calculated with a modified PowerState V12.xls. A total of 143 alleles were found in the Tibetan group with corresponding allelic frequencies ranging from 0.005 to 0.582. The observed heterozygosity, the expected heterozygosity, the power of discrimination, the power of exclusion, and the polymorphic information content ranged from 0.615 to 0.817, 0.559 to 0.787, 0.727 to 0.926, 0.310 to 0.632, and 0.488 to 0.760, respectively. Chi-square tests of the observed genotype frequencies and expected genotype frequencies in the samples showed no departure from the Hardy–Weinberg equilibrium at all loci except for D5S2500. Our results demonstrate that these 21 STRs are highly polymorphic and suitable for anthropological research, population genetics, and forensic paternity testing and human individual identification in this region, and can enrich Chinese ethnical genetic informational resources.

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References

  1. Hammond HA, Jin L, Zhong Y, Caskey CT, Chakraborty R (1994) Evaluation of 13 short tandem repeat loci for use in personal identification applications. Am J Hum Genet 55:175–189

    PubMed  CAS  Google Scholar 

  2. Zhu BF, Wang ZY, Yang CH, Li XS, Zhu J, Yang G, Huang P, Liu Y (2005) Y-chromosomal STR haplotypes in Chinese Uigur ethnic group. Int J Legal Med 119:306–309

    Article  PubMed  Google Scholar 

  3. Pitterl F, Schmidt K, Huber G, Zimmermann B, Delport R, Amory S, Ludes B, Oberacher H, Parson W (2010) Increasing the discrimination power of forensic STR testing by employing high-performance mass spectrometry, as illustrated in indigenous South African and Central Asian populations. Int J Legal Med (in press)

  4. Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506–513

    PubMed  CAS  Google Scholar 

  5. Bär W, Brinkmann B, Budowle B, Carracedo A, Gill P, Lincoln P, Mayr W, Olaisen B (1997) DNA recommendations. further report of the DNA commission of the ISFH regarding the use of short tandem repeat systems. Int J Leg Med 110:175–176

    Article  Google Scholar 

  6. Tereba A (1999) Tools for analysis of population statistics: profiles in DNA, vol 2. Promega Corporation, pp 203

  7. Nei M, Roychoudhury AK (1974) Sampling variances of heterozygosity and genetic distance. Genetics 76:379–390

    PubMed  CAS  Google Scholar 

  8. Li Y, Jiang H, Liu J, Hou L, Zhao GQ (2009) Allele frequencies for 15 STR loci in Mongolian population from Heilongjiang. J Forensic Med 24:276–278 (in Chinese)

    Google Scholar 

  9. Ota M, Droma Y, Basnyat B, Katsuyama Y, Asamura H, Sakai H, Fukuhsima H (2007) Allele frequencies for 15 STR loci in Tibetan populations from Nepal. Forensic Sci Int 169:234–238

    Article  PubMed  CAS  Google Scholar 

  10. Yan J, Shen C, Li Y, Yu X, Xiong X, Mu H, Huang Y, Deng Y, Yu J (2007) Genetic analysis of 15 STR loci on Chinese Tibetan in Qinghai Province. Forensic Sci Int 169:e3–e6

    Article  PubMed  CAS  Google Scholar 

  11. Deng Y, Zhu B, Yu X, Li Y, Fang J, Xiong X, Mu H, Huang Y, Shi X (2007) Genetic polymorphisms of 15 STR loci of Chinese Dongxiang and Salar ethnic minority living in Qinghai Province of China. Leg Med (Tokyo) 9:38–42

    CAS  Google Scholar 

  12. Zhu B, Yan J, Shen C, Li T, Li Y, Yu X, Xiong X, Mu H, Huang Y, Deng Y (2008) Population genetic analysis of 15 STR loci of Chinese Tu ethnic minority group. Forensic Sci Int 174:255–258

    Article  PubMed  CAS  Google Scholar 

  13. Xu L, Li SF, Deng QY, Zhou LN, Gong JC, Xu R (2007) Polymorphism of 15 STR loci in Maonan minority of Guangxi. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 24:180–183

    CAS  Google Scholar 

  14. Chen L, He Y, Li S (2006) Genetic analysis of 15 STR loci of Chinese Ewenki ethnic population. J Forensic Sci 51:1408–1409

    Article  PubMed  CAS  Google Scholar 

  15. Zhu BF, Shen CM, Wu QJ, Deng YJ (2008) Population data of 15 STR loci of Chinese Yi ethnic minority group. Leg Med (Tokyo) 10:220–224

    CAS  Google Scholar 

  16. Zhu B, Wang Z, Wu Q, Sedike ME, Zhu J, Huang P, Xu Y, Liu Y (2005) Genetic analysis of 15 STR loci of Chinese Uigur ethnic population. J Forensic Sci 50:1235–1236

    PubMed  CAS  Google Scholar 

  17. Hu SP, Wu DQ, Xu XH, Liu JW, Li B (2005) Genetic profile of 15 STR loci in the Min Nan population in Southeast China. Forensic Sci Int 152:263–265

    Article  PubMed  CAS  Google Scholar 

  18. Wu YM, Zhang XN, Zhou Y, Chen ZY, Wang XB (2008) Genetic polymorphisms of 15 STR loci in Chinese Han population living in Xi'an city of Shaanxi Province. Forensic Sci Int Genet 2:e15–e18

    Article  PubMed  Google Scholar 

  19. Zhu J, Li J, Guo Y, Liu K, Zhu B, Liu Y (2005) Population data of 15 STR in Chinese Han population from north of Guangdong. J Forensic Sci 50:1510–1511

    PubMed  CAS  Google Scholar 

  20. Lu DJ, Liu QL, Zhao H (2010) Genetic data of nine non-CODIS STRs in Chinese Han population from Guangdong Province, Southern China. Int J Legal Med (in press)

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Acknowledgments

This project was supported by the National Natural Science Foundation of China (NSFC, no. 30700470, 30973366, 81072511) and the Item of Science Technology Foundation of Shaanxi Province, People’s Republic China (no. 2008 K08-02).

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Authors and Affiliations

  1. The Key Laboratory of Environment and Gene Related to Diseases, Ministry of Education, Xi’an Jiaotong University, Xi’an, Shaanxi, 710061, People’s Republic of China

    Bo-feng Zhu & Xin-she Liu

  2. Blood Center of Shaanxi Province, Xi’an, Shaanxi, 710061, People’s Republic of China

    Bo-feng Zhu & Chun-mei Shen

  3. Department of Forensic Sciences, School of Medicine, Xi’an Jiaotong University, Xi’an, Shaanxi, 710061, People’s Republic of China

    Bo-feng Zhu, Hong-dan Wang, Jing-feng Huang, Hang Jing & Xin-she Liu

  4. Vascular Biology Center, Medical College of Georgia, Augusta, GA, 30912, USA

    Guang Yang

  5. Beijing Institute of Genomics, Chinese Academy of Science, Beijing, 100029, People’s Republic of China

    Jiang-wei Yan

  6. Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA, 30912, USA

    Hai-xia Qin

  7. Medical Imaging Center, First Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an, Shaanxi, 710061, People’s Republic of China

    Jian-xin Guo

Authors
  1. Bo-feng Zhu
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  2. Chun-mei Shen
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  3. Hong-dan Wang
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  4. Guang Yang
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  5. Jiang-wei Yan
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  7. Jian-xin Guo
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  8. Jing-feng Huang
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  9. Hang Jing
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  10. Xin-she Liu
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Corresponding author

Correspondence to Xin-she Liu.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary Table 1

Allelic frequencies and forensic statistical parameters regarding 21 STR loci of a Chinese Tibetan ethnic group in Lhasa city, Tibet Autonomous Region of China (XLS 188 kb)

Supplementary Table 2

The position information of 21 non-CODIS autosomal STRs (XLS 17 kb)

Supplementary Figure 1

Twenty-one STR loci and Amelogenin locus profile of a Tibetan sample (no. p137) (DOC 407 kb)

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Zhu, Bf., Shen, Cm., Wang, Hd. et al. Genetic diversities of 21 non-CODIS autosomal STRs of a Chinese Tibetan ethnic minority group in Lhasa. Int J Legal Med 125, 581–585 (2011). https://doi.org/10.1007/s00414-010-0519-4

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  • DOI: https://doi.org/10.1007/s00414-010-0519-4

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