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Quercetin attenuates lindane induced oxidative stress in wistar rats

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Abstract

A wide number of pesticides, including highly persistent organochlorine compounds, such as lindane (γ-Hexachlorocyclohexane), have deteriorative effect on fauna and flora by inducing oxidative stress. Lindane induces cell damage by producing free radicals and reactive oxygen species. Quercetin, a dietary flavonoid, is ubiquitous in fruits and vegetables and plays an important role in human health by virtue of its antioxidant function. In this study the flavonoid quercetin was used to investigate its antioxidative effect against lindane induced oxidative stress in rats. The level of lipid peroxidation, reduced glutathione (GSH) were analysed in addition to the antioxidant enzymes such as catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) and glutathione-s-transferase (GST) activities in the liver and kidney tissue. Levels of hepatic marker enzymes in serum like Aspartate transaminase (AST), Alanine transaminase (ALT), Alkaline phosphatase (ALP) and Lactate dehydrogenase (LDH) and renal markers like serum creatinine and serum urea were estimated. Administration of Lindane induced histopathological alterations and increased levels of serum hepatic and renal markers and malondialdehyde (MDA) with a significant decrease in GSH content and CAT, SOD, GPx and GST activities. Cotreatment of quercetin along with lindane significantly decreased the lindane induced alteration in histology, serum hepatic and renal markers and MDA and also improved the cellular antioxidant status. The results show that Quercetin ameliorates Lindane induced oxidative stress in liver and kidney. The quercetin exhibited chemopreventive effect when administered along with lindane.

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Abbreviations

ALP:

Alkaline phosphatase

ALT:

Alanine transaminase

ANOVA:

Analysis of variance

AST:

Aspartate transaminase

CAT:

Catalase

CPCSEA:

Committee for the purpose of control and supervision of experiments and animals

GPx:

Glutathione peroxidase

GSH:

Reduced glutathione

GST:

Glutathione-s-transferase

H&E:

Hematoxylin and eosin

LDH:

Lactate dehydrogenase

LPO:

Lipid peroxidation

MDA:

Malondialdehyde

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

TBARS:

Thio barbituric acid reactive substances

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  1. Department of Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore, 641046, Tamil Nadu, India

    Viswanadha Vijaya Padma, Rathinasamy Baskaran, Rajendra Shenoi Roopesh & Paramasivan Poornima

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  1. Viswanadha Vijaya Padma
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Correspondence to Viswanadha Vijaya Padma.

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Padma, V.V., Baskaran, R., Roopesh, R.S. et al. Quercetin attenuates lindane induced oxidative stress in wistar rats. Mol Biol Rep 39, 6895–6905 (2012). https://doi.org/10.1007/s11033-012-1516-0

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