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Catalysis of the peptide bond formation by 50 S subunits of E. Coli ribosomes with N-(formyl) methionine ester of adenylic acid as peptide donor

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Abstract

50 S subunits of E. coli ribosomes catalyze the reaction of the 2′(3′)-N-(formyl) methionine ester of adenosine 5′-phosphate and Phe-tRNA resulting in peptide bond synthesis. Cytidine 5′-phosphate stimulates this process on 50 S ribosomal subunits as well as on intact ribosomes. The obtained data show that the areas of the peptidyltransferase donor site which binds the 3′-terminal fragment of peptidyl-tRNA possess completely formed structures on 50 S ribosomal subunits.

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Authors and Affiliations

  1. Institute of Molecular Biology of the U.S.S.R. Academy of Sciences, Vavilov str., 32, 117312, Moscow, USSR

    V. V. Kotusov, M. K. Kukhanova, A. A. Krayevsky & B. P. Gottikh

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  1. V. V. Kotusov
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  2. M. K. Kukhanova
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  3. A. A. Krayevsky
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  4. B. P. Gottikh
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Kotusov, V.V., Kukhanova, M.K., Krayevsky, A.A. et al. Catalysis of the peptide bond formation by 50 S subunits of E. Coli ribosomes with N-(formyl) methionine ester of adenylic acid as peptide donor. Mol Biol Rep 3, 151–156 (1976). https://doi.org/10.1007/BF00423229

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  • DOI: https://doi.org/10.1007/BF00423229

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